Anti-inflammatory Effect of Polysaccharide from Embelia parviflora Wall.on Rheumatoid Arthritis in Rats

[Objectives]To investigate the anti-inflammatory effect of Embelia parviflora Wall.polysaccharide on rheumatoid arthritis(RA)in rats.[Methods]RA rat model was induced by type II collagen.After successful modeling,the rats were divided into model group,positive group,low,medium and high dose of E.parviflora Wall.polysaccharide groups,and normal control group.Body mass,toe volume and arthritis index were measured,and thymus index and spleen index were calculated.The levels of interleukin-1β,interleukin-6 and tumor necrosis factor-αin serum and synovial tissue of ankle joint were detected by ELISA.[Results]Compared with the normal control group,the pathological changes such as synovial hyperplasia and unclear layer were observed in the model group,the body mass was decreased(P<0.05),the toe volume,arthritis index,thymus and spleen index were increased(P<0.05),and the levels of IL-1β,IL-6 and TNF-αin serum and ankle synovial tissue were increased(P<0.05).Compared with the model group,the histopathological changes in synovium of ankle joint in the positive group and the medium and high dose groups of E.parviflora Wall.polysaccharide were significantly improved,and the body mass was increased(P<0.05).The toe volume,arthritis index,thymus index and spleen index were decreased(P<0.05).The levels of IL-Iβ,IL-6 and TNF-αin serum and synovial tissue of ankle joint were increased(P<0.05),while there was no significant difference between the low dose group of E.parviflora Wall.polysaccharide and the model group(P>0.05).[Conclusions]E.parviflora Wall.polysaccharide can reduce the body's inflammatory response and improve RA,which may be related to the inhibition of the activation of inflammatory cytokines IL-1β,IL-6 and TNF-α.

TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu

[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources.

Anti-inflammatory Mechanism of Yao Medicine Laggerae Alatae Herba

[Objectives]To study the anti-inflammatory effect of Laggerae Alatae Herba extract and its mechanism.[Methods]Inflammation models of xylene-induced ear edema in mice,acetic acid-induced increased permeability of abdominal capillaries in mice,and carrageenan-induced paw edema in mice were established;xylene-induced ear swelling model in bilateral adrenalectomized mice was established.The levels of MDA,NO and SOD in inflammatory tissues of paw were measured.[Results]Compared with the model group,the high and medium dose groups of Laggerae Alatae Herba extract had significant inhibitory effect on xylene-induced ear edema in mice,except for the low dose group(P>0.05);Laggerae Alatae Herba extract inhibited the increase of celiac capillary permeability induced by acetic acid and paw edema induced by carrageenan in mice.Compared with the model group,in the mice model with bilateral adrenal glands removed,the high and medium dose groups of Laggerae Alatae Herba extract could significantly inhibit the xylene induced ear swelling of the mice.The high and medium dose groups of Laggerae Alatae Herba extract could significantly decrease the levels of MDA and NO,and significantly increase the level of SOD in the paw tissue.[Conclusions]The Laggerae Alatae Herba extracts have anti-inflammatory activity,and the anti-inflammatory effect of the extracts does not depend on the hypothalamic-pituitary-adrenal axis(HPAA)system.In addition,the anti-inflammatory mechanism of Laggerae Alatae Herba extract is related to the decrease of MDA and NO and the increase of SOD.

TLC Identification of Yao Medicine Pileostegia tomentellal and Extraction Technology and Content Determination of Umbelliferone

[Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate(4:3)as the developing agent,and the plate was examined by UV lamp(365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm);mobile phase acetonitrile-0.2%phosphoric acid gradient elution;detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The chromatogram of P.tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27μg/mL(R^(2)=0.9997).The average recovery of umbelliferone in the low,middle and high adding groups of P.tomentellal was 99.57%and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P.tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P.tomentellal.

Research Overview of Zhuang Medicine Fumigation Lotions

At present,there is little research on the application of Zhuang medicine fumigation lotions in skin diseases,and there is no research on related preparation processes and quality standards.In this paper,the characteristics of its formulation and the research progress in skin diseases are reviewed,in order to deepen the understanding of Zhuang medicine fumigation lotions and provide a more detailed theoretical basis for its clinical research,development and application.

Pharmacodynamic Experiment of Dachengqi Decoction and Separated Decoction on Incomplete Intestinal Obstruction in Rats

[Objectives]To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats.[Methods]80 healthy SD rats were selected to establish incomplete intestinal obstruction model by silk ligation.The dosage was 20 mL/kg for 3 d,and the damage index of ileocecal mucosa was analyzed;the morphology of ileocecal mucosa was observed by HE staining;the serum levels of IL-1α,IL-1β,IL-6,IL-18,Ach,NO,ET,IL-1,TNF-αand ultra-micro Na+-K+-ATPase were detected by ELISA.[Results]Compared with the model group,the mucosal damage index of Dachengqi Decoction and each separated decoction group decreased significantly(P<0.05);compared with the normal group and sham operation group,the serum level of IL-1,IL-6,TNF-αand other factors in the model group increased significantly(P<0.05);compared with the model group,the serum IL-1,IL-6 and TNF-αsecretion levels of rats in Dachengqi Decoction group and separated decoction group decreased(P<0.01).[Conclusions]Dachengqi Decoction and each separated decoction can effectively improve intestinal tissue pathological damage in the incomplete intestinal obstruction model rats,and reduce the inflammatory reaction in the rat body.

Thin Layer Identification of Jiedu Shengxue Granules and Determination of Notoginsenoside R1 Content

[Objectives]To establish the quality standard of hospital preparation Jiedu Shengxue granules.[Methods]Scleromitrion diffusum and Prunella vulgaris in Jiedu Shengxue granules were qualitatively identified by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)was established to determine the content of notoginsenoside R1 in the granule.[Results]The traditional Chinese medicinal materials in Jiedu Shengxue granules could be identified by TLC,and the characteristic spots were stable and clear.Notoginsenoside R1 had a good linear relationship in the range of 10.45-104.5μg/mL,with an average recovery of 98.52%and RSD=2.36%.[Conclusions]TLC and HPLC,as the quality control methods of Jiedu Shengxue granules,have high accuracy and good repeatability,which lays a foundation for the quality control of this mixture.

A Preliminary Study on Effects of Zhuang Medicine Clerodendrum japonicum(Thunb.)Sweet on Acute Tonsillitis

[Objectives]To study the effect of Zhuang medicine Clerodendrum japonicum(Thunb.)Sweet on acute tonsillitis.[Methods]The antibacterial,antipyretic,anti-inflammatory and analgesic effects of the extract of Zhuang medicine C.japonicum(Thunb.)Sweet were observed.[Results]Zhuang medicine C.japonicum(Thunb.)Sweet showed a good inhibitory effect onα-hemolytic streptococcus,β-hemolytic streptococcs,Staphylococcus citreus,Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa,Proteus and Escherichia coli;it showed a good inhibitory effect on the infection of Staphylococcus aureus andβ-hemolytic streptococcs in vivo;it could significantly reduce the elevated body temperature of rabbits injected with the triple vaccine and significantly increase the pain threshold of thermally stimulated mice.[Conclusions]Zhuang Medicine C.japonicum(Thunb.)Sweet has positive significance in the treatment of acute tonsillitis instead of antibiotics.

Advances in Research of Epidemic Lung Preventive Mixture

This paper summarizes the Epidemic Lung Preventive Mixture from three aspects of research status, development trends and prospects, so as to deepen the understanding of Epidemic Lung Preventive Mixture and provide a more detailed theoretical basis for its further clinical research and development.

Research Progress and Application Prospect of Chengyin Decoction (Granule)

Research and development of Chengyin detoxification prescription are taken as research objects. Starting from preparation research and development, clinical research, urgency and market demand, and significance for industrial development, the relevant research of Chengyin detoxification prescription is systematically reviewed, and the application prospect of the ethnic medicine preparations is looked forward to.

Extraction Process of Zhuang Medicine Fumigation Lotion

[Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amount of water added,decocting time and extraction times as factors,and syringin content and dry extract yield as indexes.The content of syringin was determined by high performance liquid chromatography.[Results]The best water extraction process was:soaking in water for 1 h,decocting twice,added 10 times the amount of water each time,decocting for 1 h.The average content of syringin in 3 batches was 0.98 mg/g,and the average dry extract yield was 26.07%.[Conclusions]The project adopts water extraction method to prepare Zhuang medicine fumigation lotion,which has the characteristics of high efficiency and suitable for large-scale production.The quality control method is reliable,rapid and accurate,and can effectively control the quality of the lotion.

Research Progress in Anti-tumor Effects of Traditional Chinese Medicine and Its Extracts

Traditional Chinese medicine and its extracts have received widespread attention due to their significant anti-tumor effects and low adverse reactions.Many traditional Chinese medicine and its extracts have been applied in clinical tumor treatment.In this paper,the research progress in anti-tumor effects of plant-based traditional Chinese medicine and its extracts,animal-based traditional Chinese medicine and its extracts,and traditional Chinese medicine compound is reviewed.

Preliminary Study on Quality Analysis of Yao Medicine Pileostegia tomentella Hand.-Mazz.

[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alcohol-soluble extract of P.tomentella Hand.-Mazz.were determined.[Results]10 batches of P.tomentella Hand.-Mazz.from different habitats and in different collection time in Guangxi all had the same plant morphology and medicinal properties.The experimental results showed that the water content was 7.92%-10.35%,the total ash content was 5.53%-6.62%,the acid-insoluble ash content was 0.59%-0.76%,and the extract content was 11.63%-16.12%.[Conclusions]The identification method in this study can be used for the quality analysis of P.tomentella Hand.-Mazz.,and the experimental results can provide a scientific basis for the development and utilization of P.tomentella Hand.-Mazz.and the establishment of its quality standard.