AIM:To investigate the epidemiology and characteristics of Barrett’s esophagus(BE)in China and compare with cases in the west.METHODS:Studies were retrieved from the China National Knowledge Infrastructure and PubMed...AIM:To investigate the epidemiology and characteristics of Barrett’s esophagus(BE)in China and compare with cases in the west.METHODS:Studies were retrieved from the China National Knowledge Infrastructure and PubMed databases using the terms"Barrett"and"Barrett AND China",respectively,as well as published studies about BE in China from 2000 to 2011.The researchers reviewed the titles and abstracts of all search results to determine whether or not the literature was relevant to the current topic of this research.The references listed in the studies were also searched.Inclusion and exclusion criteria for the literature were appropriately established,and the data reported in the selected studies were analyzed.Finally,a meta-analysis was performed.RESULTS:The current research included 3873 cases of BE from 69 studies.The endoscopic detection rate of BE in China was 1%.The ratio of male to female cases was 1.781 to 1,and the average age of BE patients was 49.07±5.09 years.Island-type and shortsegment BE were the most common endoscopic manifestations,accounting for 4.48%and 80.3%,respectively,of all cases studied.Cardiac-type BE was observed in 40.0%of the cases,representing the most common histological characteristic of the condition.Cancer incidence was 1.418 per 1000 person-years.CONCLUSION:Average age of BE patients in China is lower than in Western countries.Endoscopic detection and cancer incidence were also lower in China.展开更多
AIM:To investigate the correlation between lumican(LUM)gene and high myopia in a Southern Chinese population.METHODS:The study comprised of 95 high myopia patients with a spherical equivalent≤-6.5 diopters(D).T...AIM:To investigate the correlation between lumican(LUM)gene and high myopia in a Southern Chinese population.METHODS:The study comprised of 95 high myopia patients with a spherical equivalent≤-6.5 diopters(D).The control group recruited 95 individuals with a spherical equivalent ranging from-0.5 D to+0.5 D.Direct sequencing was used to detect the single nucleotide polymorphisms(SNPs)of LUM gene in coding region.Genotype distributions were tested for Hardy-Weinberg disequilibrium.Genotypic and allelic frequencies were analyzed through Chi-square test or Fisher’s exact test.RESULTS:We identified 3 SNPs of the LUM gene:LUM c.32(rs577456426),LUM c.507(rs17853500)and LUM c.849(rs181915277).Among the three SNPs,the genotype and allele frequencies of rs17853500 showed a significant difference between patients and control subjects(P〈0.05).However,there were no significant differences in rs181915277and rs577456426 between the two groups(P〉0.05).CONCLUSION:LUM c.507 polymorphism may be a risk factor for the pathogenesis of high myopia in the Southern Chinese population.展开更多
AIM:To observe effects of trabeculectomy with amniotic membrane transplantation(AMT) in controlling postoperative intraocular pressure(IOP) in patients with medically uncontrolled glaucoma.·METHODS:This study inc...AIM:To observe effects of trabeculectomy with amniotic membrane transplantation(AMT) in controlling postoperative intraocular pressure(IOP) in patients with medically uncontrolled glaucoma.·METHODS:This study included adult patients with requiring bilateral glaucoma surgery.Each patient underwent trabeculectomy(Non-AMT group) in one eye and with AMT(AMT group) in the other eye according to randomized principle.Success was defined as intraocular pressure(IOP) 【21mmHg without any anti-glaucoma medications at 24 months follow-up.The two groups were compared in terms of IOP,complications and success rate.·RESULTS:Thirty-four eyes of 17 patients were investigated in this study.There was no statistically signifcant difference in pre-operative IOP between the two groups.The mean IOP was lower in AMT group compared with Non-AMT group on follow up months 12,18,and 24.Postoperative complications were more frequent in Non-AMT group(35.3%,6/17) compared with AMT group(5.9%,1/17).The success rate of surgery was88.2%(15/17) in Non-AMT group and 100%(17/17) in AMT group.·CONCLUSION:Trabeculectomy with AMT is an effective procedure to reduce IOP and complications,thereby improving surgical success rates.展开更多
It has become a research hotspot to explore raw material substitutes of concrete.It is important to research the mechanical properties of self-compacting concrete(SCC)with slag powder(SP)and rubber particle(RP)replaci...It has become a research hotspot to explore raw material substitutes of concrete.It is important to research the mechanical properties of self-compacting concrete(SCC)with slag powder(SP)and rubber particle(RP)replacing cement and coarse aggregate,respectively.12 kinds of composite modified self-compacting concrete(CMSCC)specimens were prepared by using 10%,20%and 30%SP and 30%,40%,50%and 60%RP.The rheological properties,mechanical properties and microstructure of the CMSCC were investigated.Results indicate that the workability,compressive strength,splitting tensile strength and flexural strength of CMSCC prepared by 20%SP and less than 40%RP are improved.In order to maximize the utilization of waste materials,20%SP and 40%RP can be used as the optimal ratio of the combined modifier.The microstructure shows that the addition of proper amount of SP is conducive to the formation of increasingly more uniform C-S-H gel.C-SH gel can fill the internal pores of the sample and enhance the adhesion between the aggregate,thus improving the mechanical properties of CMSCC.RP has a rougher surface and lower density and stiffness,which inhibits the workability and mechanical properties of CMSCC.The above research results have important theoretical and practical significance for the selection of raw materials of self-compacting concrete and the rational use of industrial wastes.展开更多
AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to co...AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized and amplified by polymerase chain reaction (PCR). The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown- multiple cloning site (MCS)-/internal ribozyme entry site (IRES)/enhanced green fluorescent protein (EGFP). Then the desired DNA fragments were integrated into the destination vector pAV.Desld yielding the final expression constructs pAV.Exld-CMV〉wt-lumican/IRES/ EGFP and pAV.Exld-cytomegalovirus (CMV) 〉mutlumican/IRES/EGFP, respectively.RESULTS: The adenovirus plasmids pAV.Exld-CMV〉 wt-lumican/IRES/EGFP and pAV.Exld-CMV 〉mutlumican/IRESlEGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION: We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumicangene in the pathogenesis of high myopia.展开更多
In this study, the density of metastable He2* in an atmospheric-pressure plasma jet operating in helium with 0.001% nitrogen has been measured using an auxiliary measuring electrode technique.In the glow discharge mod...In this study, the density of metastable He2* in an atmospheric-pressure plasma jet operating in helium with 0.001% nitrogen has been measured using an auxiliary measuring electrode technique.In the glow discharge mode, waveforms from two grounding electrodes, including one main discharge electrode and one auxiliary electrode, are captured.The isolated current peak formed by Penning ionization in waveforms from the auxiliary measuring electrode is identified to calculate the density of metastable He2*.In our discharge environment, the helium metastable densities along the jet axis direction are between 2.26×1013 and 1.74×1013 cm-3,which is in good agreement with the results measured by other techniques.This measurement technique can be conveniently applied to the diagnosis of metastable He2* in an atmospheric-pressure plasma jet array.展开更多
AIM:To compare conventional slow equilibrium cooling and directional freezing(DF) by gauze package for cryopreservation of human umbilical vein endothelial cells(HUVECs).METHODS:HUVECs were randomly assigned to conven...AIM:To compare conventional slow equilibrium cooling and directional freezing(DF) by gauze package for cryopreservation of human umbilical vein endothelial cells(HUVECs).METHODS:HUVECs were randomly assigned to conventional freezing(CF) and DF by gauze package group. The two groups of HUVECs were incubated with a freezing liquid consisting of 10% dimethylsulfoxide(DMSO), 60% fetal bovine serum(FBS) and 30%Dulbecco’s modified Eagle’s medium(DMEM) and then put into cryopreserved tubes. CF group, slow equilibrium cooling was performed with the following program:precool in 4℃ for 30 min,-20℃ for 1h, and then immersion in-80℃ refrigerator. DF group, the tubes were packaged with gauze and then directional freezing in-80℃ refrigerator straightly. One month later, the vitality of HUVECs were calculated between two groups.RESULTS:There was no significant difference in the survival rate and growth curve between CF and DF groups. The DF group was significantly better than CFgroup in adherent rates, morphological changes and proliferative ability.CONCLUSION:In the conventional cryopreserved method, cells are slow equilibrium cooling by steps(4℃,-20℃ and finally-80℃), which is a complicated and time-consuming process. But the improved DF by gauze package method is better than conventional method, for which is convenient and easy to operate.展开更多
AIM: To investigate the association of lysyl oxidaselike 1(LOXL1) single nucleotide polymorphisms(SNPs)with exfoliation syndrome(XFS)/exfoliation glaucoma(XFG).METHODS: Published manuscripts from Pub Med and EMBASE we...AIM: To investigate the association of lysyl oxidaselike 1(LOXL1) single nucleotide polymorphisms(SNPs)with exfoliation syndrome(XFS)/exfoliation glaucoma(XFG).METHODS: Published manuscripts from Pub Med and EMBASE were identified until May 2014. Summary odds ratios(ORs) and 95% confidence intervals(CIs) for LOXL1(rs1048661, rs2165241 and rs3825942) polymorphisms and the risk of XFS/XFG were estimated using random-or fixed- effect model.· RESULTS: The three LOXL1 polymorphisms(rs1048661, rs3825942, and rs2165241) were associated with an increased risk for XFS/XFG among Caucasians,with OR 2.19(1.96-2.45), 8.8(6.05-12.79) and 3.41(3.11-3.73), respectively. On the contrast, the rs1048661 and rs2165241, but not rs3825942 polymorphism, have a potential protective effect on XFS/XFG in Asians, with OR0.06(0.02-0.18), 0.15(0.09-0.25), respectively.CONCLUSION: There is strong evidence that LOXL1 polymorphisms are associated with XFS/XFG risk. The strength of risk might be ethnicity-dependent.展开更多
Apoptosis,,a wide physiological process in multicel-lular organisms, is critical for the organ development, the cell senescence, the tissue homeostasis and the resistance to infection of virus and bacteria. Apoptosis ...Apoptosis,,a wide physiological process in multicel-lular organisms, is critical for the organ development, the cell senescence, the tissue homeostasis and the resistance to infection of virus and bacteria. Apoptosis plays a key role in the regulation of the growth cell number such as the tissue construction, the elimination of abnormal or dangerous cells. Therefore, apoptosis is a stringent and effective cell quality controlling system, which reduces the number of harmful cells to the maximum, such as auto-immunity cell, cells infected by virus, tumor cells by cell suicide111. Apoptosis can be initiated by a wide variety of the extracellular and intracellular stimuli, including the developmental signals, the cellular stress and the disruption of cell cycle, transduced and amplified by the second messengers, and finally finished by the activating death effector protease. This process can be called apoptosis signal network. The defective in control of the apoptotic pathways may contribute to a variety展开更多
This paper presents a demand dispatch strategy of aggregated electric water heaters(EWHs) for a load aggregation system at demand side, based on the theory of cyber-physical system. The objective is to solve the probl...This paper presents a demand dispatch strategy of aggregated electric water heaters(EWHs) for a load aggregation system at demand side, based on the theory of cyber-physical system. The objective is to solve the problem of water heater load control when the cyber-physical load aggregation system participates in demand dispatch of the power grid. First, an implementation framework of the demand dispatch strategy is designed between the cyber space and the physical space, including state awareness,real-time analysis, scientific decision-making and precise execution. Second, a multilevel incentive model, an EWH appliance model and a thermostat setpoint control rule are introduced. Next, based on the models and the rule, the state awareness logic, real-time analysis logic, scientific decision-making logic and precise execution logic of the strategy are designed to implement demand dispatch of aggregated EWHs. Finally, simulation results confirm the effectiveness, the advantage and excellent scalability of the proposed strategy.展开更多
Paired amphipathic helix protein (SIN3B) is a transcription corepressor for many genes. Here we show a different regulation mechanism of integrin aV gene expression by SIN3B in human hepatocellular carcinoma (HCC). We...Paired amphipathic helix protein (SIN3B) is a transcription corepressor for many genes. Here we show a different regulation mechanism of integrin aV gene expression by SIN3B in human hepatocellular carcinoma (HCC). We first observed a close relationship between Integrin aV and SIN3B expressions in HCC patients and tumor cell lines with different metastatic potentials. Overexpression of SIN3B significantly accelerated the cell migration rate of SMMC-7721, but failed when integrin αV expression was silenced. Interestingly, SIN3B stimulated integrin aV subunit promoter activity only in the presence of sulfatide. Importantly, SIN3B was identified in the complex with sulfatide by mass spectrometry. Fat blot assay indicated that SIN3B specifically interacted with sulfatide. Molecular modeling suggested that sulfatide induced the conformational change of SIN3B from compacted α-helices to a relaxed β-sheet in PAH2 domain. The data of immunoprecipitation and ChIP assay indicated that altered SIN3B lost the binding affinity with MAD1 and HDAC2, which reduced the recruitment of HDAC2 on integrin aV gene promoter and prevented the deacetylation of the histone 3. In conclusion, this study demonstrated that SIN3B promoted the transcriptional activation of the integrin αV subunit gene promoter by reducing interaction with HDAC2.展开更多
文摘AIM:To investigate the epidemiology and characteristics of Barrett’s esophagus(BE)in China and compare with cases in the west.METHODS:Studies were retrieved from the China National Knowledge Infrastructure and PubMed databases using the terms"Barrett"and"Barrett AND China",respectively,as well as published studies about BE in China from 2000 to 2011.The researchers reviewed the titles and abstracts of all search results to determine whether or not the literature was relevant to the current topic of this research.The references listed in the studies were also searched.Inclusion and exclusion criteria for the literature were appropriately established,and the data reported in the selected studies were analyzed.Finally,a meta-analysis was performed.RESULTS:The current research included 3873 cases of BE from 69 studies.The endoscopic detection rate of BE in China was 1%.The ratio of male to female cases was 1.781 to 1,and the average age of BE patients was 49.07±5.09 years.Island-type and shortsegment BE were the most common endoscopic manifestations,accounting for 4.48%and 80.3%,respectively,of all cases studied.Cardiac-type BE was observed in 40.0%of the cases,representing the most common histological characteristic of the condition.Cancer incidence was 1.418 per 1000 person-years.CONCLUSION:Average age of BE patients in China is lower than in Western countries.Endoscopic detection and cancer incidence were also lower in China.
基金Supported by Natural Science Foundation of Guangdong Province,China(No.2015A030310158No.2014A030313359)+1 种基金the Science and Technology Planning Project of Guangdong Province,China(No.2015B020226003)the Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University(No.2015201)
文摘AIM:To investigate the correlation between lumican(LUM)gene and high myopia in a Southern Chinese population.METHODS:The study comprised of 95 high myopia patients with a spherical equivalent≤-6.5 diopters(D).The control group recruited 95 individuals with a spherical equivalent ranging from-0.5 D to+0.5 D.Direct sequencing was used to detect the single nucleotide polymorphisms(SNPs)of LUM gene in coding region.Genotype distributions were tested for Hardy-Weinberg disequilibrium.Genotypic and allelic frequencies were analyzed through Chi-square test or Fisher’s exact test.RESULTS:We identified 3 SNPs of the LUM gene:LUM c.32(rs577456426),LUM c.507(rs17853500)and LUM c.849(rs181915277).Among the three SNPs,the genotype and allele frequencies of rs17853500 showed a significant difference between patients and control subjects(P〈0.05).However,there were no significant differences in rs181915277and rs577456426 between the two groups(P〉0.05).CONCLUSION:LUM c.507 polymorphism may be a risk factor for the pathogenesis of high myopia in the Southern Chinese population.
基金2011 Guangdong Province Natural Science Fund,China (No.S2011010004186)National Basic Research Program of China (973 program,2011CB707501)
文摘AIM:To observe effects of trabeculectomy with amniotic membrane transplantation(AMT) in controlling postoperative intraocular pressure(IOP) in patients with medically uncontrolled glaucoma.·METHODS:This study included adult patients with requiring bilateral glaucoma surgery.Each patient underwent trabeculectomy(Non-AMT group) in one eye and with AMT(AMT group) in the other eye according to randomized principle.Success was defined as intraocular pressure(IOP) 【21mmHg without any anti-glaucoma medications at 24 months follow-up.The two groups were compared in terms of IOP,complications and success rate.·RESULTS:Thirty-four eyes of 17 patients were investigated in this study.There was no statistically signifcant difference in pre-operative IOP between the two groups.The mean IOP was lower in AMT group compared with Non-AMT group on follow up months 12,18,and 24.Postoperative complications were more frequent in Non-AMT group(35.3%,6/17) compared with AMT group(5.9%,1/17).The success rate of surgery was88.2%(15/17) in Non-AMT group and 100%(17/17) in AMT group.·CONCLUSION:Trabeculectomy with AMT is an effective procedure to reduce IOP and complications,thereby improving surgical success rates.
基金This research was supported by the Key Science and Technology Projects in Transportation Industry(2018-MS2-051).
文摘It has become a research hotspot to explore raw material substitutes of concrete.It is important to research the mechanical properties of self-compacting concrete(SCC)with slag powder(SP)and rubber particle(RP)replacing cement and coarse aggregate,respectively.12 kinds of composite modified self-compacting concrete(CMSCC)specimens were prepared by using 10%,20%and 30%SP and 30%,40%,50%and 60%RP.The rheological properties,mechanical properties and microstructure of the CMSCC were investigated.Results indicate that the workability,compressive strength,splitting tensile strength and flexural strength of CMSCC prepared by 20%SP and less than 40%RP are improved.In order to maximize the utilization of waste materials,20%SP and 40%RP can be used as the optimal ratio of the combined modifier.The microstructure shows that the addition of proper amount of SP is conducive to the formation of increasingly more uniform C-S-H gel.C-SH gel can fill the internal pores of the sample and enhance the adhesion between the aggregate,thus improving the mechanical properties of CMSCC.RP has a rougher surface and lower density and stiffness,which inhibits the workability and mechanical properties of CMSCC.The above research results have important theoretical and practical significance for the selection of raw materials of self-compacting concrete and the rational use of industrial wastes.
基金Supported by the Natural Science Foundation of Guangdong Province(No.2015A030310158No.2014A030313359)+1 种基金the Fundamental Research Funds for the Central Universities(No.21611446)the Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University(No.2015201)
文摘AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized and amplified by polymerase chain reaction (PCR). The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown- multiple cloning site (MCS)-/internal ribozyme entry site (IRES)/enhanced green fluorescent protein (EGFP). Then the desired DNA fragments were integrated into the destination vector pAV.Desld yielding the final expression constructs pAV.Exld-CMV〉wt-lumican/IRES/ EGFP and pAV.Exld-cytomegalovirus (CMV) 〉mutlumican/IRES/EGFP, respectively.RESULTS: The adenovirus plasmids pAV.Exld-CMV〉 wt-lumican/IRES/EGFP and pAV.Exld-CMV 〉mutlumican/IRESlEGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION: We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumicangene in the pathogenesis of high myopia.
基金supported by National Natural Science Foundation of China (No.11105093)
文摘In this study, the density of metastable He2* in an atmospheric-pressure plasma jet operating in helium with 0.001% nitrogen has been measured using an auxiliary measuring electrode technique.In the glow discharge mode, waveforms from two grounding electrodes, including one main discharge electrode and one auxiliary electrode, are captured.The isolated current peak formed by Penning ionization in waveforms from the auxiliary measuring electrode is identified to calculate the density of metastable He2*.In our discharge environment, the helium metastable densities along the jet axis direction are between 2.26×1013 and 1.74×1013 cm-3,which is in good agreement with the results measured by other techniques.This measurement technique can be conveniently applied to the diagnosis of metastable He2* in an atmospheric-pressure plasma jet array.
基金Supported by Science and Technology Foundation of Zhuhai(No.PB200510142013D0401990017)
文摘AIM:To compare conventional slow equilibrium cooling and directional freezing(DF) by gauze package for cryopreservation of human umbilical vein endothelial cells(HUVECs).METHODS:HUVECs were randomly assigned to conventional freezing(CF) and DF by gauze package group. The two groups of HUVECs were incubated with a freezing liquid consisting of 10% dimethylsulfoxide(DMSO), 60% fetal bovine serum(FBS) and 30%Dulbecco’s modified Eagle’s medium(DMEM) and then put into cryopreserved tubes. CF group, slow equilibrium cooling was performed with the following program:precool in 4℃ for 30 min,-20℃ for 1h, and then immersion in-80℃ refrigerator. DF group, the tubes were packaged with gauze and then directional freezing in-80℃ refrigerator straightly. One month later, the vitality of HUVECs were calculated between two groups.RESULTS:There was no significant difference in the survival rate and growth curve between CF and DF groups. The DF group was significantly better than CFgroup in adherent rates, morphological changes and proliferative ability.CONCLUSION:In the conventional cryopreserved method, cells are slow equilibrium cooling by steps(4℃,-20℃ and finally-80℃), which is a complicated and time-consuming process. But the improved DF by gauze package method is better than conventional method, for which is convenient and easy to operate.
基金Supported by Natural Science Foundation of Guangdong Province(No.S2013010016037)the National Science and Tecnology Plan Project of China(973 program,No.2011CB707501)
文摘AIM: To investigate the association of lysyl oxidaselike 1(LOXL1) single nucleotide polymorphisms(SNPs)with exfoliation syndrome(XFS)/exfoliation glaucoma(XFG).METHODS: Published manuscripts from Pub Med and EMBASE were identified until May 2014. Summary odds ratios(ORs) and 95% confidence intervals(CIs) for LOXL1(rs1048661, rs2165241 and rs3825942) polymorphisms and the risk of XFS/XFG were estimated using random-or fixed- effect model.· RESULTS: The three LOXL1 polymorphisms(rs1048661, rs3825942, and rs2165241) were associated with an increased risk for XFS/XFG among Caucasians,with OR 2.19(1.96-2.45), 8.8(6.05-12.79) and 3.41(3.11-3.73), respectively. On the contrast, the rs1048661 and rs2165241, but not rs3825942 polymorphism, have a potential protective effect on XFS/XFG in Asians, with OR0.06(0.02-0.18), 0.15(0.09-0.25), respectively.CONCLUSION: There is strong evidence that LOXL1 polymorphisms are associated with XFS/XFG risk. The strength of risk might be ethnicity-dependent.
文摘Apoptosis,,a wide physiological process in multicel-lular organisms, is critical for the organ development, the cell senescence, the tissue homeostasis and the resistance to infection of virus and bacteria. Apoptosis plays a key role in the regulation of the growth cell number such as the tissue construction, the elimination of abnormal or dangerous cells. Therefore, apoptosis is a stringent and effective cell quality controlling system, which reduces the number of harmful cells to the maximum, such as auto-immunity cell, cells infected by virus, tumor cells by cell suicide111. Apoptosis can be initiated by a wide variety of the extracellular and intracellular stimuli, including the developmental signals, the cellular stress and the disruption of cell cycle, transduced and amplified by the second messengers, and finally finished by the activating death effector protease. This process can be called apoptosis signal network. The defective in control of the apoptotic pathways may contribute to a variety
基金supported by National Natural Science Foundation of China(No.51777068)Fundamental Research Funds for the Central Universities(No.2016XS15)
文摘This paper presents a demand dispatch strategy of aggregated electric water heaters(EWHs) for a load aggregation system at demand side, based on the theory of cyber-physical system. The objective is to solve the problem of water heater load control when the cyber-physical load aggregation system participates in demand dispatch of the power grid. First, an implementation framework of the demand dispatch strategy is designed between the cyber space and the physical space, including state awareness,real-time analysis, scientific decision-making and precise execution. Second, a multilevel incentive model, an EWH appliance model and a thermostat setpoint control rule are introduced. Next, based on the models and the rule, the state awareness logic, real-time analysis logic, scientific decision-making logic and precise execution logic of the strategy are designed to implement demand dispatch of aggregated EWHs. Finally, simulation results confirm the effectiveness, the advantage and excellent scalability of the proposed strategy.
文摘Paired amphipathic helix protein (SIN3B) is a transcription corepressor for many genes. Here we show a different regulation mechanism of integrin aV gene expression by SIN3B in human hepatocellular carcinoma (HCC). We first observed a close relationship between Integrin aV and SIN3B expressions in HCC patients and tumor cell lines with different metastatic potentials. Overexpression of SIN3B significantly accelerated the cell migration rate of SMMC-7721, but failed when integrin αV expression was silenced. Interestingly, SIN3B stimulated integrin aV subunit promoter activity only in the presence of sulfatide. Importantly, SIN3B was identified in the complex with sulfatide by mass spectrometry. Fat blot assay indicated that SIN3B specifically interacted with sulfatide. Molecular modeling suggested that sulfatide induced the conformational change of SIN3B from compacted α-helices to a relaxed β-sheet in PAH2 domain. The data of immunoprecipitation and ChIP assay indicated that altered SIN3B lost the binding affinity with MAD1 and HDAC2, which reduced the recruitment of HDAC2 on integrin aV gene promoter and prevented the deacetylation of the histone 3. In conclusion, this study demonstrated that SIN3B promoted the transcriptional activation of the integrin αV subunit gene promoter by reducing interaction with HDAC2.