Differences of cyanide leaching between calcine and dust from refractory gold concentrates

Differences of cyanide leaching between the calcine and the dust from a refractory gold concentrate were investigated by comparative method. Results showed that gold leaching efficiencies of the calcine and the dust were 85.31% and 54.30%, respectively, with direct cyanidation. Contents and existing forms of arsenic and carbon were the main reasons for those differences. The maximum gold leaching efficiencies of the calcine and the dust were 87.70% and 58.60%, respectively, with cyanidation after NaOH pre-leaching. Harmful elements removal, gold loss in NaOH pre-leaching and iron oxides hindrance codetermined gold leaching efficiencies of the calcine and the dust. After H2SO4 pre-leaching, the maximum gold leaching efficiencies of the calcine and the dust achieved 94.96% and 80.40%, respectively. The effect of carbonaceous matter was the main reason for differences for leaching efficiencies of the calcine and the dust. Based on those differences, two proper gold extraction processes were put forward, and gold leaching efficiencies for the calcine and the dust achieved 94.91% and 91.90%, respectively.

An analysis of the transcriptional landscape in hypoxia-treated primary nucleus pulposus cells

Intervertebral disc degeneration(IDD)is the main cause of lower back pain.Lower back pain places a huge burden on society,and all current treatments for IDD cannot restore the original function of the intervertebral disc.Proposing new treatments for IDD requires clarifying the mechanisms of IDD.Physiological hypoxia is an important feature of the nucleus pulposus because of its special anatomical structure.Using RNA sequencing(RNA-seq),we obtained the whole transcriptome of nucleus pulposus cells(NPCs)under hypoxia and hypoxia-inducible factor(HIF1A)deletion.Results demonstrated possible effects of oxygen concentration and HIF1A on NPCs.In addition,our results showed that hypoxia can affect lipid metabolism in NPCs.

Genetic diversity and temporal dynamics of phytoplankton viruses in East Lake, China

Phytoplankton viruses are important components of aquatic ecosystems. However, their prevalence and genetic diversity in marine and freshwater systems are largely under estimated owing to the immense size of water bodies and limitations in virus discovery techniques. In this study, we conducted a 1-year survey of phytoplankton virus communities by collecting surface water monthly from an inland lake(East Lake) in China between May 2012 and April 2013. We examined four phytoplankton viruses, i.e., myoviruses, podoviruses, siphoviruses, and phycodnaviruses, and seven sets of primers were used to target conserved genes within these four species. In this year-long investigation, a total of 358 different virus-related sequences from four virus families were obtained. All virus families were detected in all months, except for cyanopodoviruses, which were only identified during eight of the 12 months surveyed. Moreover, virus abundance and diversity changed dynamically over time. Phylogenetic analysis revealed that the majority of viral sequences from East Lake, China displayed distinct clustering patterns compared with published sequences. These results supported the existence of a highly diverse and unique phytoplankton virus community in East Lake, China.

Development and utilization of a new chemically-induced soybean library with a high mutation density

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

Strong stability of an optimal control hybrid system in fed-batch fermentation

In this paper, we consider a nonlinear hybrid dynamic （NHD） system to describe fedbatch culture where there is no analytical solutions and no equilibrium points. Our goal is to prove the strong stability with respect to initial state for the NHD system. To this end, we construct corresponding linear variational system （LVS） for the solution of the NHD system, also prove the boundedness of fundamental matrix solutions for the LVS. On this basis, the strong stability is proved by such boundedness.

总体国家安全观视野下监狱刑罚执行工作的挑战与应对

党的十八大以来,习近平总书记创造性地提出总体国家安全观系统思想,总体国家安全观成为维护国家安全的行动纲领、科学指南和根本遵循。作为国家刑罚执行机关,监狱是社会公共安全的减压阀,是国家刑事法律的出泻口,是化解社会尖锐矛盾的主战场,监狱本身的安全及其职能的发挥程度,影响社会和谐稳定,事关国家总体安全。新的刑事法律政策颁布实施后,监狱面临一些困境与挑战,有必要在总体国家安全观视野下探寻保障监管安全、规范刑罚执行、强化教育改造的新途径。