Correlation of BRD4 and Skp2 expression levels in ultrasound-guided thyroid nodule fine needle aspiration biopsy tissue with the pathological features of nodules

Objective:To study the correlation of bromodomain-containing protein 4(BRD4)and S-phase kinase-associated protein 2(Skp2)expression levels in ultrasound-guided thyroid nodule fine needle aspiration biopsy tissue with the pathological features of nodules.Methods:The tissues obtained in ultrasound-guided thyroid nodule fine needle aspiration biopsy in our hospital between March 2015 and March 2018 was selected and divided into malignant group and benign group according to the pathological results,and the expression levels of BRD4,Skp2,proliferation genes and angiogenesis genes were detected.Results:The BRD4 and Skp2 mRNA expression in thyroid nodules of the malignant group were significantly higher than those of the benign group,and the BRD4 and Skp2 mRNA expression in the malignant group of thyroid nodules with TNM III-IV,capsular invasion and lymph node metastasis were significantly higher than those in the thyroid nodules with TNM I-II,without capsular invasion and without lymph node metastasis;cyclin D1(CCND1),β-catenin,proliferation cell nuclear antigen(PCNA),vascular endothelial growth factor(VEGF),endothelial cell specific molecule-1(ESM-1),Survivin and cyclooxygenase 2(COX2)mRNA expression in thyroid nodules of the malignant group were obviously higher than those of the benign group and positively correlated with BRD4 and Skp2 while cyclin G2(CCNG2)and endostatin(ES)mRNA expression were significantly lower than those of the benign group and negatively correlated with BRD4 and Skp2.Conclusion:The high expression of BRD4 and Skp2 in malignant thyroid nodules is correlated with the pathological changes and can change the expression of proliferation genes and angiogenesis genes.

Effect of Xiaoaiping combined with neoadjuvant chemotherapy on the pro-proliferation molecule expression and immune function in patients with breast cancer

Objective: To investigate the effect of Xiaoaiping combined with neoadjuvant chemotherapy on the pro-proliferation molecule expression and immune function in patients with breast cancer. Methods: A total of 98 patients with primary breast cancer who were diagnosed and treated in the hospital between December 2015 and February 2017 were collected and divided into control group and Xiaoaiping group by random number table, each with 49 cases. Control group received neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy, and Xiaoaiping group received Xiaoaiping + neoadjuvant chemotherapy + surgery + postoperative chemoradiotherapy. The differences in pro-proliferation gene expression in intraoperative breast cancer tissue as well as the differences in serum levels of Th1/Th2 cytokines and Th17/Treg cytokines before chemotherapy started (T0) and 1 week after neoadjuvant chemotherapy ended (T1) were compared between the two groups of patients. Results: MTA2, NRP-1, PKM2, TM4SF1 and ZIC1 mRNA expression in breast cancer tissue of Xiaoaiping group were lower than those of control group. At T1, serum IFN-γ and TNF-α levels of Xiaoaiping group were higher than those of control group whereas IL-4, IL-10, IL-17, IL-22, IL-35 and TGF-β levels were lower than those of control group. Conclusion: Xiaoaiping combined with neoadjuvant chemotherapy can effectively improve the curative effect of preoperative chemotherapy, and also significantly inhibit the proliferation activity of breast cancer cells and balance the immune function of the body.