Parametric Problems in Power System Analysis:Recent Applications of Polynomial Approximation Based on Galerkin Method

In power systems, there are many uncertainty factors such as power outputs of distributed generations and fluctuations of loads. It is very beneficial to power system analysis to acquire an explicit function describing the relationship between these factors(namely parameters) and power system states(or performances). This problem, termed as parametric problem(PP) in this paper, can be solved by Galerkin method,which is a powerful and mathematically rigorous method aiming to seek an accurate explicit approximate function by projection techniques. This paper provides a review of the applications of polynomial approximation based on Galerkin method in power system PPs as well as stochastic problems. First, the fundamentals of polynomial approximation and Galerkin method are introduced. Then, the process of solving three types of typical PPs by polynomial approximation based on Galerkin method is elaborated. Finally, some application examples as well as several potential applications of power system PPs solved by Galerkin method are presented, namely the probabilistic power flow, approximation of static voltage stability region boundary, and parametric time-domain dynamic simulation.

Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton

Spinosyns are natural broad-spectrum biological insecticides with a double glycosylated polyketide structure that are produced by aerobic fermentation of the actinomycete,Saccharopolyspora spinosa.However,their large-scale overproduction is hindered by poorly understood bottlenecks in optimizing the original strain,and poor adaptability of the heterologous strain to the production of spinosyn.In this study,we genetically engineered heterologous spinosyn-producer Streptomyces albus J1074 and optimized the fermentation to improve the production of spinosad(spinosyn A and spinosyn D)based on our previous work.We systematically investigated the result of overexpressing polyketide synthase genes(spnA,B,C,D,E)using a constitutive promoter on the spinosad titer in S.albus J1074.The supply of polyketide synthase precursors was then increased to further improve spinosad production.Finally,increasing or replacing the carbon source of the culture medium resulted in a final spinosad titer of~70 mg/L,which is the highest titer of spinosad achieved in heterologous Streptomyces species.This research provides useful strategies for efficient heterologous production of natural products.

Kinetics of antimony biogeochemical processes under pre-definite anaerobic and aerobic conditions in a paddy soil

While the transformation of antimony(Sb) in paddy soil has been previously investigated, the biogeochemical processes of highly chemical active Sb in the soil remain poorly understood. In addition, there is a lack of quantitative understanding of Sb transformation in soil. Therefore, in this study, the kinetics of exogenous Sb in paddy soils were investigated under anaerobic and aerobic incubation conditions. The dissolved Sb(V) and the Sb(V) extracted by diffusive gradient technique decreased under anaerobic conditions and then increased under aerobic conditions. The redox reaction of Sb occurred, and Sb bioavailability significantly decreased after 55 days of incubation. The kinetics of Fe and the scanning transmission electron microscopy analysis revealed that the Fe oxides were reduced and became dispersed under anaerobic conditions, whereas they were oxidized and re-aggregated during the aerobic stage. In addition, the redox processes of sulfur and nitrogen were detected under both anaerobic and aerobic conditions. Based on these observations, a simplified kinetic model was established to distinguish the relative contributions of the transformation processes. The bioavailability of Sb was controlled by immobilization as a result of S reduction and by mobilization as a result of Fe reductive dissolution and S oxidation, rather than the p H. These processes coupled with the redox reaction of Sb jointly resulted in the complex behavior of Sb transformation under anaerobic and aerobic conditions. The model-based method and findings of this study provide a comprehensive understanding of the Sb transformation in a complex soil biogeochemical system under changing redox conditions.

Why is the SARS-CoV-2 Omicron variant milder?

In November 2021,a new SARS-CoV-2 variant emerged in South Africa and was designated the fifth variant of concern and named Omicron.It was detected in more than 26 countries worldwide,including China.The Omicron variant is highly transmissible and has extensive morbidity,which has raised concerns related to antiviral therapy.Recent reports have revealed that the Omicron variant exhibits a longer cycle of viral shedding and a decreased replication capacity and results in substantially attenuated lung pathology,indicating that the pathogenic ability of the Omicron variant is lower than that of previous variants.1Omicron has the largest number(>30)of substitutions,deletions,or insertions,with a mutation frequency of higher than 1%in the spike(S)protein.Mutation studies of the spike protein receptor-binding domain have provided a plausible explanation for the increased transmissibility and antibody resistance of this variant.However,the molecular basis for its attenuated pathogenicity and replication capacity remains elusive.