The pre-analytical steps in blood-based liquid biopsy,involving sample collection techniques and storage conditions,play a critical role in ensuring the integrity and reliability of collected samples.These steps have ...The pre-analytical steps in blood-based liquid biopsy,involving sample collection techniques and storage conditions,play a critical role in ensuring the integrity and reliability of collected samples.These steps have a directly impact on the accuracy and reliability of test results and are therefore of utmost importance.Mass spectrometry(MS)-based proteomics is an exceptionally powerful tool in the field of liquid biopsy.It enables the comprehensive analysis of the protein content within biological specimens,providing valuable insights into the underlying mechanisms and pathogenesis of diseases.In this study,we aim to explore the variations in the protein landscape between serum and plasma specimens and evaluate the impact of delayed centrifugation on LC/MS-analyzed protein profiles.We seek to provide recommendations on optimal pre-analytical protocols for MS-based proteomics studies.This will enhance the accuracy and reliability of liquid biopsy for precision medicine,ultimately leading to improved patient outcomes.展开更多
What is already known about this topic?Many health challenges have emerged due to rapid population aging,including declined cognitive ability among older adults.What is added by this report?Childhood circumstances hav...What is already known about this topic?Many health challenges have emerged due to rapid population aging,including declined cognitive ability among older adults.What is added by this report?Childhood circumstances have significant and lasting impacts on cognition in old age.This study compared cognition data from China with both the United States(U.S.)and the European Union(EU)during 2008–2018,finding that childhood circumstances could respectively explain 65.4%[95%confidence interval(CI):59.4%,71.4%](China vs.the U.S.)and 38.2%(95%CI:35.1%,41.2%)(China vs.the EU)of the overall differences in cognition among older adults.Family socioeconomic status explained the largest share of differences among all considered childhood circumstances.What are the implications for public health practice?Large disparities in cognition should be addressed by mitigating childhood disadvantages.展开更多
基金the financial support from the National Natural Science Foundation of China(22274029 for C.Zhu)the Science and Technology Commission of Shanghai Municipality(22ZR1412000 for C.Zhu22140900700 and 23ZR1411300 for Q.Yao).
文摘The pre-analytical steps in blood-based liquid biopsy,involving sample collection techniques and storage conditions,play a critical role in ensuring the integrity and reliability of collected samples.These steps have a directly impact on the accuracy and reliability of test results and are therefore of utmost importance.Mass spectrometry(MS)-based proteomics is an exceptionally powerful tool in the field of liquid biopsy.It enables the comprehensive analysis of the protein content within biological specimens,providing valuable insights into the underlying mechanisms and pathogenesis of diseases.In this study,we aim to explore the variations in the protein landscape between serum and plasma specimens and evaluate the impact of delayed centrifugation on LC/MS-analyzed protein profiles.We seek to provide recommendations on optimal pre-analytical protocols for MS-based proteomics studies.This will enhance the accuracy and reliability of liquid biopsy for precision medicine,ultimately leading to improved patient outcomes.
基金Supported by the National Natural Science Foundation of China(71974097)a Career Development Award(K01AG053408)+1 种基金a major research grant(R01AG077529)he Claude D.Pepper Older Americans Independence Center at Yale School of Medicine(P30AG021342)funded by the National Institute on Aging,Jiangsu Qinglan Project,Jiangsu the Priority Academic Program Development of Jiangsu Higher Education Institute(PAPD).
文摘What is already known about this topic?Many health challenges have emerged due to rapid population aging,including declined cognitive ability among older adults.What is added by this report?Childhood circumstances have significant and lasting impacts on cognition in old age.This study compared cognition data from China with both the United States(U.S.)and the European Union(EU)during 2008–2018,finding that childhood circumstances could respectively explain 65.4%[95%confidence interval(CI):59.4%,71.4%](China vs.the U.S.)and 38.2%(95%CI:35.1%,41.2%)(China vs.the EU)of the overall differences in cognition among older adults.Family socioeconomic status explained the largest share of differences among all considered childhood circumstances.What are the implications for public health practice?Large disparities in cognition should be addressed by mitigating childhood disadvantages.
