The endophytic fungi in different tissues of Artemisia annua was isolated and purified to explore their ecological distribution and tissue preference, and the extracellular enzyme activities of dominant endophytic fun...The endophytic fungi in different tissues of Artemisia annua was isolated and purified to explore their ecological distribution and tissue preference, and the extracellular enzyme activities of dominant endophytic fungi were determined to characterize the metabolic function of endophytic fungi. The results showed that a total of 67 endophytic fungi were obtained from Artemisia annua tissues. The number and species of endophytic fungi in different tissues were significantly different. The number, colonization rate (CR) and isolation rate (IR) of endophytic fungi in root were significantly higher than those of stem and leaf. The dominant endophytic fungi, diversity and similarity coefficient of endophytic fungi also showed significant difference among tissues. The extracellular enzyme activities of endophytic fungi in different tissues are significantly different. The enzyme activities of endophytic fungi isolated from root are significantly higher than those isolated from stem and leaf. The research results showed that the endophytic fungi in Artemisia annua had significant tissue preference, and the metabolic function of endophytic fungi showed significant difference among tissues. This will lay a foundation for further research, development and utilization of endophytic fungi, and also provide a theoretical basis for screening functional endophytic fungi in Artemisia annua.展开更多
Fungi are the key agents in litter decomposition in forest ecosystems. However, the specific roles of the interactions between different fungal species during litter decomposition process are unclear. To evaluate the ...Fungi are the key agents in litter decomposition in forest ecosystems. However, the specific roles of the interactions between different fungal species during litter decomposition process are unclear. To evaluate the interactions, two fungi strains with significantly different morphs were isolated from the soils of Quercus acutissima forest and Pinus massoniana forest, and inoculated in the litter powder of Quercus acutissima leaves and Pinus massoniana needles with grown separately and in coexistence equally through a microcosm experiment. The enzyme activities were determined as a proxy for microbial activities. The results showed that the degradative enzymes involved in litter decomposition showed varying dynamics pattern during the incubation period. The interactions between the two fungi strains are synergism, and benefit to each other according to enzyme activities, suggesting that a fungi strain growth was accelerated by the presence of other fungi strain during litter decomposition process. However, the interactions of the two fungi strains were bilateral antagonism inoculated in the litter powder of Quercus acutissima leaves according to cellobiohydrolase activities. The synergism, despite bilateral antagonism in an exceptional case, may be an important factor controlling the fungal colonization and growth on litter substrate. The results implied that more fungal species may accelerate litter decomposition rates due to their mutual cooperation.展开更多
Background:The benefits of laparoscopic gastrectomy(LG)in elderly gastric-cancer patients still remain unclear.The purpose of this study was to evaluate the feasibility and safety of LG in elderly gastric-cancer patie...Background:The benefits of laparoscopic gastrectomy(LG)in elderly gastric-cancer patients still remain unclear.The purpose of this study was to evaluate the feasibility and safety of LG in elderly gastric-cancer patients.Methods:We retrospectively evaluated patients who underwent LG or open gastrectomy(OG)between June 2009 and July 2015 in a single high-volume center.We compared surgical,short-term,and long-termsurvival outcomes among an elderly(-70 years old)LG(ELG)group(n=114),a non-elderly(<70 years old)LG(NLG)group(n=740),and an elderly OG(EOG)group(n=383).Results:Except for extended time to first flatus,the surgical and short-term outcomes of the ELG group were similar to those of the NLG group.The ELG group revealed comparable disease-specific survival(DSS)rates to the NLG group(64.9%vs 66.2%,P=0.476),although the overall survival(OS)rate was lower(57.0%vs 65.5%,P<0.001)in the ELG group than in the NLG group.The ELG group showed longer operation time than the EOG group(236.4677.3 vs 179652.2 min,P<0.001).The ELG group had less estimated blood loss(174.0688.4 vs 209.36133.8,P=0.008)and shorter post-operative hospital stay(8.362.5 vs 9.264.5,P=0.048)than the EOG group.The severity of complications was similar between the ELG and NLG groups.Multivariate analysis confirmed that LG was not a risk factor for post-operative complications.Conclusions:LG is a feasible and safe procedure for elderly patients with acceptable short-and long-term survival outcomes.展开更多
文摘The endophytic fungi in different tissues of Artemisia annua was isolated and purified to explore their ecological distribution and tissue preference, and the extracellular enzyme activities of dominant endophytic fungi were determined to characterize the metabolic function of endophytic fungi. The results showed that a total of 67 endophytic fungi were obtained from Artemisia annua tissues. The number and species of endophytic fungi in different tissues were significantly different. The number, colonization rate (CR) and isolation rate (IR) of endophytic fungi in root were significantly higher than those of stem and leaf. The dominant endophytic fungi, diversity and similarity coefficient of endophytic fungi also showed significant difference among tissues. The extracellular enzyme activities of endophytic fungi in different tissues are significantly different. The enzyme activities of endophytic fungi isolated from root are significantly higher than those isolated from stem and leaf. The research results showed that the endophytic fungi in Artemisia annua had significant tissue preference, and the metabolic function of endophytic fungi showed significant difference among tissues. This will lay a foundation for further research, development and utilization of endophytic fungi, and also provide a theoretical basis for screening functional endophytic fungi in Artemisia annua.
文摘Fungi are the key agents in litter decomposition in forest ecosystems. However, the specific roles of the interactions between different fungal species during litter decomposition process are unclear. To evaluate the interactions, two fungi strains with significantly different morphs were isolated from the soils of Quercus acutissima forest and Pinus massoniana forest, and inoculated in the litter powder of Quercus acutissima leaves and Pinus massoniana needles with grown separately and in coexistence equally through a microcosm experiment. The enzyme activities were determined as a proxy for microbial activities. The results showed that the degradative enzymes involved in litter decomposition showed varying dynamics pattern during the incubation period. The interactions between the two fungi strains are synergism, and benefit to each other according to enzyme activities, suggesting that a fungi strain growth was accelerated by the presence of other fungi strain during litter decomposition process. However, the interactions of the two fungi strains were bilateral antagonism inoculated in the litter powder of Quercus acutissima leaves according to cellobiohydrolase activities. The synergism, despite bilateral antagonism in an exceptional case, may be an important factor controlling the fungal colonization and growth on litter substrate. The results implied that more fungal species may accelerate litter decomposition rates due to their mutual cooperation.
基金supported by the National Key Research and Development Program of China [2017YFC1311004]The Key Research and Development Program of Shanxi province [2017-ZDXM-SF-053].
文摘Background:The benefits of laparoscopic gastrectomy(LG)in elderly gastric-cancer patients still remain unclear.The purpose of this study was to evaluate the feasibility and safety of LG in elderly gastric-cancer patients.Methods:We retrospectively evaluated patients who underwent LG or open gastrectomy(OG)between June 2009 and July 2015 in a single high-volume center.We compared surgical,short-term,and long-termsurvival outcomes among an elderly(-70 years old)LG(ELG)group(n=114),a non-elderly(<70 years old)LG(NLG)group(n=740),and an elderly OG(EOG)group(n=383).Results:Except for extended time to first flatus,the surgical and short-term outcomes of the ELG group were similar to those of the NLG group.The ELG group revealed comparable disease-specific survival(DSS)rates to the NLG group(64.9%vs 66.2%,P=0.476),although the overall survival(OS)rate was lower(57.0%vs 65.5%,P<0.001)in the ELG group than in the NLG group.The ELG group showed longer operation time than the EOG group(236.4677.3 vs 179652.2 min,P<0.001).The ELG group had less estimated blood loss(174.0688.4 vs 209.36133.8,P=0.008)and shorter post-operative hospital stay(8.362.5 vs 9.264.5,P=0.048)than the EOG group.The severity of complications was similar between the ELG and NLG groups.Multivariate analysis confirmed that LG was not a risk factor for post-operative complications.Conclusions:LG is a feasible and safe procedure for elderly patients with acceptable short-and long-term survival outcomes.
