Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T.urticae.In this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on T.urticae and its predator Neoseiulus californicus using the highly puri?ed compound.Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) method.When the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days post-application.At concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the molt.However,neither concentration had any adverse effects on the survival or reproduction of the predatory mite N.californicus.The results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

数智赋能智慧教育的变与不变之道

数智技术的出现,为教育带来新的可能性,将持续推动教育领域的深刻变革。在数智技术赋能教育数字化转型的背景下,智慧教育代表着未来教育发展的趋势。智慧教育借助数智技术的力量,跨越传统课堂教学的界限,引领着教育领域向全新的学习模式和教育观念转变。基于这样的时代背景,厘清技术影响下教育的发展规律,探讨数智赋能智慧教育的变与不变之道,有助于数智技术高效地推动教育的向好发展。首先,解构教育数字化转型背景下教育的变与不变,厘清这个问题,有助于教育从业者更好地坚守初心、把握机遇、顺应变革。然后,在深入论述智慧教育带来教育之变的基础上,探讨智慧教育的未来范式,即人机协同学习、高意识学习、智慧学习生态化和新质人才培养范式。最后,提出应对数智时代教育变革的策略:教育契约从集权管理向民主治理转变;教育文化从自囿因袭向开放创新转变;思维品质从固化心态向成长心态转变;教学模式从教为中心向学为中心转变;教育目标从知识本位向能力本位转变;供需关系从供给主导向需求主导转变;教育规制从阶段学习向终身学习转变;教育架构从谱系结构向泛在生态转变。

Responses of Annual Variability of Vegetation NPP to Climate Variables Using Satellite Techniques in Gadarif State, Sudan

Plants play an essential role in matter and energy transformations and are key messengers in the carbon and energy cycle. Net primary productivity (NPP) reflects the capability of plants to transform solar energy into photosynthesis. It is very sensible for factors affecting on vegetation variability such as climate, soils, plant characteristics and human activities. So, it can be used as an indicator of actual and potential trend of vegetation. In this study we used the actual NPP which was derived from MODIS to assess the response of NPP to climate variables in Gadarif State, from 2000 to 2010. The correlations between NPP and climate variables (temperature and precipitation) are calculated using Pearson’s Correlation Coefficient and ordinary least squares regression. The main results show the following 1) the correlation Coefficient between NPP and mean annual temperature is Somewhat negative for Feshaga, Rahd, Gadarif and Galabat areas and weakly negative in Faw area;2) the correlation Coefficient between NPP and annual total precipitation is weakly negative in Faw, Rahd and Galabat areas and somewhat negative in Galabat and Rahd areas. This study demonstrated that the correlation analysis between NPP and climate variables (precipitation and temperature) gives reliably result of NPP responses to climate variables that is clearly in a very large scale of study area.

Enhancing Hydrocarbon-Rich Bio-Oil Production via Catalytic Pyrolysis Fortified with Microorganism Pretreatment

A new method of pretreatment of corn straw with Phanerochaete chrysosporium combined with pyrolysis was proposed to improve the quality of bio-oil.The characterization results demonstrated that microbial pretreatment was an effective method to decrease the lignin,which can achieve a maximum removal rate of 44.19%.Due to the destruction of biomass structure,the content of alkali metal and alkaline earth metal is reduced.Meanwhile,the depolymerized biomass structure created better pyrolysis conditions to promote the pyrolysis efficiency,increase the average decomposition rate of pyrolysis and reduce the residue.In fast pyrolysis,because of the enrichment of cellulose and the removal of lignin,the contents of acids,linear carbonyls,furans,and sugars increased while the contents of phenols decreased.As for the catalytic pyrolysis,due to the hydrocarbon pool reaction and shape selection deoxidation of ZSM-5 catalyst,the total hydrocarbon and aromatics contents can significantly increase up to 34.37%and 30.59%,respectively,with 3 weeks of pretreatment.And the the low molecular content of bio-oil increased after pretreatment,which can significantly benefit the entry of primary pyrolysis steam into the catalyst pores to improve the catalytic efficiency and hydrocarbon contents.This method provides a new treatment idea for high-quality utilization of biomass.

A preliminary study on the acoustic properties of seafloor sediment in the southern U-boundary of the South China Sea

The acoustic properties of seafloor sediment are essential parameters in the exploration of marine resources,ocean scientific research and ocean engineering.Seafloor sediment samples were collected at the southern U-boundary of the South China Sea(SCS),and the acoustic and physical properties were measured in the laboratory.The correlation between physical and sound speed ratio(SSR)was discussed,and SSR-physical property empirical regressions in the Sunda Shelf were established for the first time.Compared with the northern continental shelf of SCS,the Sunda Shelf are mainly silty and sand sediment,and the SSR ranges from 0.9949 to 1.0944,which has higher SSR than the northern continental shelf,implies that the Sunda Shelf is a high SSR area.Since the same kind of sediment has different physical properties,the single physical parameter of sediment cannot fully represent the acoustic properties of sediment,therefore,the multiple parameter prediction model should develop in the future to improve the prediction precision.

基于活性炭‖Na_(0.44)MnO_(2)的低成本、高倍率和长寿命碱性钠离子电池电容器

水系钠离子电池电容器具有成本低、功率大、安全性好等优点,是下一代大规模储能系统的理想选择之一。本文采用Na_(0.44)MnO_(2)正极、活性炭(AC)负极、6mol·L^(-1)NaOH电解液和廉价的不锈钢集流体构建了可充电碱性钠离子电池电容器。由于Na_(0.44)MnO_(2)正极在碱性电解液中具有较高的过充耐受性,通过首次充电时的原位过充预活化过程可以解决半钠化Na_(0.44)MnO_(2)正极和AC负极初始库伦效率低的缺点。因此,AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具有优异的电化学性能,在功率密度为85 W·kg^(-1)时,能量密度达26.6 Wh·kg^(-1),循环10000次后容量保持率为89%。同时,在50℃的高温和-20℃的低温也具有良好的电化学性能。这些结果表明AC‖Na_(0.44)MnO_(2)可充电碱性钠离子电池电容器具备应用于大规模储能的潜力。

Efficacy and safety of endoscopic submucosal dissection for early gastric cancer and precancerous lesions in elderly patients

BACKGROUND With advancements in the development of endoscopic technologies,the endo-scopic submucosal dissection(ESD)has been one of the gold-standard therapies for early gastric cancer.AIM To investigate the efficacy and safety ESD in the treatment of early gastric cancer and precancerous lesions in the elderly patients.METHODS Seventy-eight elderly patients with early gastric cancer and precancerous lesions admitted to the Third Affiliated Hospital of Qiqihar Medical University were se-lected and classified into two groups according to the different surgical therapies they received between January 2021 and June 2022.Among them,39 patients treated with ESD were included in an experimental group,and 39 patients treated with endoscopic mucosal resection(EMR)were included in a control group.We compared the basic intraoperative conditions,postoperative short-term recovery,long-term recovery effects and functional status of gastric mucosa between the two groups;the basic intraoperative conditions included lesion resection,intra-operative bleeding and operation time;the postoperative short-term recovery assessment indexes were length of hospital stay and incidence of surgical complic-ations;and the long-term recovery assessment indexes were the recurrence rate at 1 year postoperatively and the survival situation at 1 year and 3 years postoper-atively;and we compared the preoperative and predischarge serum pepsinogen I(PG I)and PG II levels and PG I/PG II ratio in the two groups before surgery and discharge.RESULTS The curative resection rate and the rate of en bloc resection were higher in the experimental group than in the control group.The intraoperative bleeding volume was higher in the experimental group than in the control group.The operation time was longer in the experimental group than that in the control group,and the rate for base residual focus was lower in the experimental group than that of the control group,and the differences were all statistically significant(all P<0.05).The length of hospital stay was longer in the experi-mental group than in the control group,and the incidence of surgical complications,1-year postoperative recu-rrence rate and 3-year postoperative survival rate were lower in the experimental group than in the control group,and the differences were statistically significant(all P<0.05).However,the difference in the 1-year postoperative survival rate was not statistically significant between the two groups(P>0.05).Before discharge,PG I and PG I/PG II ratio were elevated in both groups compared with the preoperative period,and the above indexes were higher in the experimental group than those in the control group,and the differences were statistically significant(both P<0.05).Moreover,before discharge,PG II level was lower in both groups compared with the preoperative period,and the level was lower in the experimental group than in the control group,and the differences were all statistically significant(all P<0.05).CONCLUSION Compared with EMR,ESD surgery is more thorough.It reduces the rate of base residual focus,recurrence rate,surgical complications,and promotes the recovery of gastric cells and glandular function.It is safe and suitable for clinical application.

Development of a convolutional neural network based geomechanical upscaling technique for heterogeneous geological reservoir

Geomechanical assessment using coupled reservoir-geomechanical simulation is becoming increasingly important for analyzing the potential geomechanical risks in subsurface geological developments.However,a robust and efficient geomechanical upscaling technique for heterogeneous geological reservoirs is lacking to advance the applications of three-dimensional(3D)reservoir-scale geomechanical simulation considering detailed geological heterogeneities.Here,we develop convolutional neural network(CNN)proxies that reproduce the anisotropic nonlinear geomechanical response caused by lithological heterogeneity,and compute upscaled geomechanical properties from CNN proxies.The CNN proxies are trained using a large dataset of randomly generated spatially correlated sand-shale realizations as inputs and simulation results of their macroscopic geomechanical response as outputs.The trained CNN models can provide the upscaled shear strength(R^(2)>0.949),stress-strain behavior(R^(2)>0.925),and volumetric strain changes(R^(2)>0.958)that highly agree with the numerical simulation results while saving over two orders of magnitude of computational time.This is a major advantage in computing the upscaled geomechanical properties directly from geological realizations without the need to perform local numerical simulations to obtain the geomechanical response.The proposed CNN proxybased upscaling technique has the ability to(1)bridge the gap between the fine-scale geocellular models considering geological uncertainties and computationally efficient geomechanical models used to assess the geomechanical risks of large-scale subsurface development,and(2)improve the efficiency of numerical upscaling techniques that rely on local numerical simulations,leading to significantly increased computational time for uncertainty quantification using numerous geological realizations.

Age-specific differences in the association between prediabetes and cardiovascular diseases in China:A national cross-sectional study

BACKGROUND Cardiovascular disease(CVD)is a leading cause of morbidity and mortality worldwide,the global burden of which is rising.It is still unclear the extent to which prediabetes contributes to the risk of CVD in various age brackets among adults.To develop a focused screening plan and treatment for Chinese adults with prediabetes,it is crucial to identify variations in the connection between prediabetes and the risk of CVD based on age.AIM To examine the clinical features of prediabetes and identify risk factors for CVD in different age groups in China.METHODS The cross-sectional study involved a total of 46239 participants from June 2007 through May 2008.A thorough evaluation was conducted.Individuals with prediabetes were categorized into two groups based on age.Chinese atherosclerotic CVD risk prediction model was employed to evaluate the risk of developing CVD over 10 years.Random forest was established in both age groups.SHapley Additive exPlanation method prioritized the importance of features from the perspective of assessment contribution.RESULTS In total,6948 people were diagnosed with prediabetes in this study.In prediabetes,prevalences of CVD were 5(0.29%)in the younger group and 148(2.85%)in the older group.Overall,11.11%of the younger group and 29.59% of the older group were intermediate/high-risk of CVD for prediabetes without CVD based on the Prediction for ASCVD Risk in China equation in ten years.In the younger age group,the 10-year risk of CVD was found to be more closely linked to family history of CVD rather than lifestyle,whereas in the older age group,resident status was more closely linked.CONCLUSION The susceptibility to CVD is age-specific in newly diagnosed prediabetes.It is necessary to develop targeted approaches for the prevention and management of CVD in adults across various age brackets.

有机-无机杂化材料动力学与热力学转化以及荧光信息防伪应用研究——介绍一个综合化学实验

有机-无机杂化发光材料易于调控的空间结构和发光性质,在发光防伪领域备受青睐。本实验基于Sb(Ⅲ)掺杂Bi(Ⅲ)基零维有机-无机杂化金属卤化物,利用不同的晶体生长方式实现动力学产物(化合物1)向热力学产物(化合物2)的转变,采用X-射线粉末衍射和荧光光度法分别对其进行了物相表征和光学性能测试,二者表现出截然不同的物相与光物理性质。化合物2在蒸馏水的作用下实现荧光淬灭,而且具有很强的反水性。基于化合物2出色的发光变色性质,制作了简易的可水写荧光信息防伪板/纸。本实验充分体现了“结构决定性质”的专业理论、“学以致用”的教学理念。该实验设计与实践旨在培养学生创新思维,提高其分析和解决实际问题的能力。

Experimental investigation on shear strength deterioration at the interface between different rock types under cyclic loading

The shear strength deterioration of bedding planes between different rock types induced by cyclic loading is vital to reasonably evaluate the stability of soft and hard interbedded bedding rock slopes under earthquake;however,rare work has been devoted to this subject due to lack of attention.In this study,experimental investigations on shear strength weakening of discontinuities with different joint wall material(DDJM)under cyclic loading were conducted by taking the interface between siltstone and mudstone in the Shaba slope of Yunnan Province,China as research objects.A total of 99 pairs of similar material samples of DDJM(81 pairs)and discontinuities with identical joint wall material(DIJM)(18 pairs)were fabricated by inserting plates,engraved with typical surface morphology obtained by performing three-dimensional laser scanning on natural DDJMs sampled from field,into mold boxes.Cyclic shear tests were conducted on these samples to study their shear strength changes with the cyclic number considering the effects of normal stress,joint surface morphology,shear displacement amplitude and shear rate.The results indicate that the shear stress vs.shear displacement curves under each shear cycle and the peak shear strength vs.cyclic number curves of the studied DDJMs are between those of DIJMs with siltstone and mudstone,while closer to those of DIJMs with mudstone.The peak shear strengths of DDJMs exhibit an initial rapid decline followed by a gradual decrease with the cyclic number and the decrease rate varies from 6%to 55.9%for samples with varied surface morphology under different testing conditions.The normal stress,joint surface morphology,shear displacement amplitude and shear rate collectively influence the shear strength deterioration of DDJM under cyclic shear loading,with the degree of influence being greater for larger normal stress,rougher surface morphology,larger shear displacement amplitude and faster shear rate.

An integrated MINLP model for multi-party coordination in downstream oil supply chain

Cooperation among enterprises can bring overall and individual performance improvement,and a smooth coordination method is indispensable.However,due to the lack of customized coordination methods,cooperation in the downstream oil supply chain cannot be carried out smoothly.This paper intends to propose a multi-party coordination method to promote cooperation between oil shippers and pipeline operator by optimizing oil transportation,oil substitution and pipeline pricing schemes.An integrated game-theoretic modeling and analysis approach is developed to characterize the operation behaviors of all stakeholders in the downstream oil supply chain.The proposed mixed integer nonlinear programming model constrains supply and demand capacity,transportation routes,oil substitution rules and pipeline freight levels.Logarithm transformation and price discretization are introduced for model linear approximation.Simulation experiments are carried out in the oil distribution system in South China.The results show that compared to the business-as-usual scheme,the new scheme saves transportation cost by 3.48%,increases pipeline turnover by 5.7%,and reduces energy consumption and emissions by 7.66%and 6.77%.It is proved that the proposed method improves the revenue of the whole system,achieves fair revenue distribution,and also improves the energy and environmental benefits of the oil supply chain.

Proteo-transcriptomic profiles reveal key regulatory pathways and functions of LDHA in the ovulation of domestic chickens(Gallus gallus)

Background In poultry, the smooth transition of follicles from the preovulatory-to-postovulatory phase impacts egg production in hens and can benefit the poultry industry. However, the regulatory mechanism underlying follicular ovulation in avians is a complex biological process that remains unclear.Results Critical biochemical events involved in ovulation in domestic chickens(Gallus gallus) were evaluated by transcriptomics, proteomics, and in vitro assays. Comparative transcriptome analyses of the largest preovulatory follicle(F1) and postovulatory follicle(POF1) in continuous laying(CL) and intermittent laying(IL) chickens indicated the greatest difference between CL_F1 and IL_F1, with 950 differentially expressed genes(DEGs), and the smallest difference between CL_POF1 and IL_POF1, with 14 DEGs. Additionally, data-independent acquisition proteomics revealed 252 differentially abundant proteins between CL_F1 and IL_F1. Perivitelline membrane synthesis, steroid biosynthesis, lysosomes, and oxidative phosphorylation were identified as pivotal pathways contributing to ovulation regulation. In particular, the regulation of zona pellucida sperm-binding protein 3, plasminogen activator, cathepsin A, and lactate dehydrogenase A(LDHA) was shown to be essential for ovulation. Furthermore, the inhibition of LDHA decreased cell viability and promoted apoptosis of ovarian follicles in vitro.Conclusions This study reveals several important biochemical events involved in the process of ovulation, as well as crucial role of LDHA. These findings improve our understanding of ovulation and its regulatory mechanisms in avian species.

Chemical looping reforming of the micromolecular component from biomass pyrolysis via Fe_(2)O_(3)@SBA-16

To solve the problems of low gasification efficiency and high tar content caused by solid–solid contact between biomass and oxygen carrier in traditional biomass chemical looping gasification process.The decoupling strategy was adopted to decouple the biomass gasification process,and the composite oxygen carrier was prepared by embedding Fe_(2)O_(3) in molecular sieve SBA-16 for the chemical looping reforming process of pyrolysis micromolecular model compound methane,which was expected to realize the directional reforming of pyrolysis volatiles to prepare hydrogen-rich syngas.Thermodynamic analysis of the reaction system was carried out based on the Gibbs free energy minimization method,and the reforming performance was evaluated by a fixed bed reactor,and the kinetic parameters were solved based on the gas–solid reaction model.Thermodynamic analysis verified the feasibility of the reaction and provided theoretical guidance for experimental design.The experimental results showed that the reaction performance of Fe_(2)O_(3)@SBA-16 was compared with that of pure Fe_(2)O_(3) and Fe_(2)O_(3)@SBA-15,and the syngas yield was increased by 55.3%and 20.7%respectively,and it had good cycle stability.Kinetic analysis showed that the kinetic model changed from three-dimensional diffusion to first-order reaction with the increase of temperature.The activation energy was 192.79 kJ/mol by fitting.This paper provides basic data for the directional preparation of hydrogen-rich syngas from biomass and the design of oxygen carriers for pyrolysis of all-component chemical looping reforming.

A coupled Legendre-Laguerre polynomial method with analytical integration for the Rayleigh waves in a quasicrystal layered half-space with an imperfect interface

The Laguerre polynomial method has been successfully used to investigate the dynamic responses of a half-space.However,it fails to obtain the correct stress at the interfaces in a layered half-space,especially when there are significant differences in material properties.Therefore,a coupled Legendre-Laguerre polynomial method with analytical integration is proposed.The Rayleigh waves in a one-dimensional(1D)hexagonal quasicrystal(QC)layered half-space with an imperfect interface are investigated.The correctness is validated by comparison with available results.Its computation efficiency is analyzed.The dispersion curves of the phase velocity,displacement distributions,and stress distributions are illustrated.The effects of the phonon-phason coupling and imperfect interface coefficients on the wave characteristics are investigated.Some novel findings reveal that the proposed method is highly efficient for addressing the Rayleigh waves in a QC layered half-space.It can save over 99%of the computation time.This method can be expanded to investigate waves in various layered half-spaces,including earth-layered media and surface acoustic wave(SAW)devices.

Development of a high-repetition-rate lumped-inductance kicker magnet prototype for the beam switchyard of SHINE

The Shanghai high-repetition-rate X-ray free-electron laser and extreme light facility(SHINE)operates at a maximum repetition rate of 1 MHz.Kicker magnets are key components that distribute electron bunches into three different undulator lines in a bunch-by-bunch mode.The kicker field width must be less than the time interval between bunches.A lumpedinductance kicker prototype was developed using a vacuum chamber with a single-turn coil.The full magnetic field strength was 0.005 T.This paper presents the requirements,design considerations,design parameters,magnetic field calculations,and measurements of the kicker magnets.The relevant experimental results are also presented.The pulse width of the magnetic field was approximately 600 ns,and the maximum operation repetition rate was 1 MHz.The developed kicker satisfies the requirements for the SHINE project.Finally,numerous recommendations for the future optimization of kicker magnets are provided.

Size-dependent thermomechanical vibration characteristics of rotating pre-twisted functionally graded shear deformable microbeams

A three-dimensional(3D)thermomechanical vibration model is developed for rotating pre-twisted functionally graded(FG)microbeams according to the refined shear deformation theory(RSDT)and the modified couple stress theory(MCST).The material properties are assumed to follow a power-law distribution along the chordwise direction.The model introduces one axial stretching variable and four transverse deflection variables including two pure bending components and two pure shear ones.The complex modal analysis and assumed mode methods are used to solve the governing equations of motion under different boundary conditions(BCs).Several examples are presented to verify the effectiveness of the developed model.By coupling the slenderness ratio,gradient index,rotation speed,and size effect with the pre-twisted angle,the effects of these factors on the thermomechanical vibration of the microbeam with different BCs are investigated.It is found that with the increase in the pre-twisted angle,the critical slenderness ratio and gradient index corresponding to the thermal instability of the microbeam increase,while the critical material length scale parameter(MLSP)and rotation speed decrease.The sensitivity of the fundamental frequency to temperature increases with the increasing slenderness ratio and gradient index,and decreases with the other increasing parameters.Moreover,the size effect can suppress the dynamic stiffening effect and enhance the Coriolis effect.Finally,the mode transition is quantitatively demonstrated by a modal assurance criterion(MAC).

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri:intraspecific and interspecific patterns

Lactobacillus rhamnosus(Rh)and Lactobacillus reuteri(Re)are well-known probiotic species in inflammatory bowel disease(IBD)research.The variations between these species’efficacy against colitis,and their model of action in this regard,are intriguing and enable treatment to be individually tailored to patients.In this study,four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced.The anti-colitis activities of both strains involved various aspects of intestinal immune,physical,chemical,and biological barrier function.Strikingly,the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration.Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis.Nevertheless,Re strains were more effective than Rh strains in inhibiting production of inflammatory factors;promoting production of intestinal mucus,antimicrobial peptides,and tight junction proteins;and supporting the stem cell compartment.This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains.In addition,the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism-and prophage-related genes,respectively.Taken together,the results of this study could assist researchers in developing effective therapies for IBD.

The influence of yttrium and manganese additions on the degradation and biocompatibility of magnesium-zinc-based alloys:In vitro and in vivo studies

The repair and regeneration of bone defects are highly challenging orthopedic problems.Recently,Mg-based implants have gained popularity due to their unique biodegradation and elastic modulus similar to that of human bone.The aim of our study is to develop a magnesium alloy with a controllable degradation that can closely match bone tissue to help injuries heal in vivo and avoid cytotoxicity caused by a sudden increase in ion concentration.In this study,we prepared and modified Mg-3Zn,Mg-3Zn-1Y,and Mg-2Zn-1Mn by hot extrusion,and used Mg-2.5Y-2.5Nd was as a control.We then investigated the effect of additions of Y and Mn on alloys'properties.Our results show that Mn and Y can improve not only compression strength but also corrosion resistance.The alloy Mg-2Zn-1Mn demonstrated good cytocompatibility in vitro,and for this reason we selected it for implantation in vivo.The degraded Mg-2Zn-1Mn implanted a bone defect area did not cause obvious rejection and inflammatory reaction,and the degradation products left no signs of damage to the heart,liver,kidney,or brain.Furthermore,we find that Mg-2Zn-1Mn can promote an osteoinductive response in vivo and the formation of bone regeneration.

Tumor necrosis factor α deficiency promotes myogenesis and muscle regeneration

Tumor necrosis factorα(TNFα)exhibits diverse biological functions;however,its regulatory roles in myogenesis are not fully understood.In the present study,we explored the function of TNFαin myoblast proliferation,differentiation,migration,and myotube fusion in primary myoblasts and C2C12 cells.To this end,we constructed TNFαmuscle-conditional knockout(TNFα-CKO)mice and compared them with flox mice to assess the effects of TNFαknockout on skeletal muscles.Results indicated that TNFα-CKO mice displayed phenotypes such as accelerated muscle development,enhanced regenerative capacity,and improved exercise endurance compared to flox mice,with no significant differences observed in major visceral organs or skeletal structure.Using label-free proteomic analysis,we found that TNFα-CKO altered the distribution of several muscle development-related proteins,such as Hira,Casz1,Casp7,Arhgap10,Gas1,Diaph1,Map3k20,Cfl2,and Igf2,in the nucleus and cytoplasm.Gene set enrichment analysis(GSEA)further revealed that TNFαdeficiency resulted in positive enrichment in oxidative phosphorylation and MyoD targets and negative enrichment in JAK-STAT signaling.These findings suggest that TNFα-CKO positively regulates muscle growth and development,possibly via these newly identified targets and pathways.