The development of efficient contrast agents for tumor-targeted imaging remains a critical challenge in the clinic.Herein,we proposed a tumor-derived extracellular vesicle(EV)-mediated targeting approach to improve in...The development of efficient contrast agents for tumor-targeted imaging remains a critical challenge in the clinic.Herein,we proposed a tumor-derived extracellular vesicle(EV)-mediated targeting approach to improve in vivo tumor imaging using ternary downconversion nanoparticles(DCNPs)with strong near infrared II(NIR-II)luminescence at 1,525 nm.The EVs were metabolically engineered with azide group,followed by in vivo labeling of DCNPs through copper-free click chemistry.By taking advantage of the homologous targeting property of tumor derived EVs,remarkable improvement in the tumor accumulation(6.5%injection dose(ID)/g)was achieved in the subcutaneous colorectal cancer model when compared to that of individual DCNPs via passive targeting(1.1%ID/g).Importantly,such bioorthogonal labeling significantly increased NIR-II luminescence signals and prolonged the retention at tumor sites.Our work demonstrates the great potential of EVs-mediated bioorthogonal approach for in vivo labeling of NIR-II optical probes,which provides a robust tool for tumor-specific imaging and targeted therapy.展开更多
Synthetic zircon(ZrSiO_(4))ceramics are typically fabricated at elevated temperatures(over 1500℃),which would lead to high manufacturing cost.Meanwhile,reports about preparing ZrSiO_(4)-based ceramic composites via c...Synthetic zircon(ZrSiO_(4))ceramics are typically fabricated at elevated temperatures(over 1500℃),which would lead to high manufacturing cost.Meanwhile,reports about preparing ZrSiO_(4)-based ceramic composites via controlling the solid-state reaction between zirconia(ZrO_(2))and silica(SiO_(2))are limited.In this work,we proposed a low-temperature strategy to flexibly design and fabricate ZrSiO_(4)-based ceramic composites via doping and tuning the solid-state reaction.Two ceramic composites and ZrSiO_(4) ceramics were in-situ prepared by reactive fast hot pressing(FHP)at approximately 1250℃ based on the proposed strategy,i.e.,a ZrSiO_(4)-SiO_(2) dual-phase composite with bicontinuous interpenetrating and hierarchical microstructures,a ZrSiO_(4)-ZrO_(2) dual-phase composite with a microstructure of ZrO_(2) submicron-and nano-particles embedded in a micron ZrSiO_(4) matrix,and ZrSiO_(4) ceramics with a small amount of residual ZrO_(2) nanoparticles.The results showed that the phase compositions,microstructure configurations,mechanical properties,and wear resistance of the materials can be flexibly regulated by the proposed strategy.Hence,ZrSiO_(4)-based ceramic composites with different properties can be easily fabricated based on different application scenarios.These findings would offer useful guidance for researchers to flexibly fabricate ZrSiO_(4)-based ceramic composites at low temperatures and tailor their microstructures and properties through doping and tuning the solid-state reaction.展开更多
Aging precipitation behavior and mechanical properties of Inconel 617 superalloy aged at 760℃ for up to 10000 h were investigated. The results showed that the precipitates of the aged alloy are M23C6 and M6C carbides...Aging precipitation behavior and mechanical properties of Inconel 617 superalloy aged at 760℃ for up to 10000 h were investigated. The results showed that the precipitates of the aged alloy are M23C6 and M6C carbides and γ phase. The carbide particles precipitated both at the grain boundaries and within grains, and the γ phase particles were situated at intragranular sites in the process of aging. The carbide particles were discontinuously dispersed at grain boundaries after aging for 3000 h, while after aged for 5000 h the carbide particles are merged. The precipitates inside grains remained stable even after aging for 10000 h. The hardness was increased for the alloy aged for 300 h up to 3000 h, which was resulted primarily from the precipitation of carbides as discrete particles both at the grain boundaries and inside grains. Small quantity γ precipitates were formed inside grains, to some extent, which contributed to an enhanced hardness. However, a decrease of the hardness was observed after aging for 5000 h. A significant drop in toughness of the alloy aged for 300 h was attributed to the reduction of the bonding interface strength when carbides precipitated at grain boundaries. Thereafter, the toughness decreased slowly with the prolonged aging time. The high temperature tensile properties of the aged alloy are rather stable even aged for 300-3000 h.展开更多
In applications such as parallel mesh refinement,it remains a challenging issue to ensure the refined surface respects the original Computer-Aided Design(CAD)model accurately.In this paper,an ultralight geometry proce...In applications such as parallel mesh refinement,it remains a challenging issue to ensure the refined surface respects the original Computer-Aided Design(CAD)model accurately.In this paper,an ultralight geometry processing library is developed to resolve this issue effectively and efficiently.Here,we say the kernel is ultralight because it has a very small set of data-structures and algorithms by comparison with industrial-level geometry kernels.Within the library,a simplified surface boundary representation(B-rep)and a radial edge structure are developed respectively to depict the geometry model and the surface mesh,plus hash tables that record the connections between the geometry model and the surface mesh.Based on these data structures,a set of efficient algorithms are developed,which initializes the connection tables,projects a point back to the original geometry,etc.With these data-structure and algorithmic infrastructures set up,the callings of eight well-designed Application Programming Interfaces(APIs)are powerful enough to enable the parallel mesh refinement algorithm outputs a mesh respecting the input CAD model accurately.Numerical experiments will be finally presented to evaluate the performance of the overall parallel mesh refinement algorithm and the algorithms in relation with the developed library.展开更多
基金supported by the China Postdoctoral Science Foundation(No.2022M712157)China National Postdoctoral Program for Innovative Talents(No.BX20220215)+5 种基金China Scientific Research Foundation of Peking University Shenzhen Hospital(No.KYQD202100X)the National Natural Science Foundation of China(No.32101074)Shenzhen Science and Technology Innovation Committee Discipline Layout Project(No.JCYJ20170816105345191)National University of Singapore Start-up Grant(No.NUHSRO/2020/133/Startup/08)NUS School of Medicine Nanomedicine Translational Research Programme(No.NUHSRO/2021/034/TRP/09/Nanomedicine)the National Medical Research Council(NMRC)Centre Grant Programme(No.CG21APR1005).
文摘The development of efficient contrast agents for tumor-targeted imaging remains a critical challenge in the clinic.Herein,we proposed a tumor-derived extracellular vesicle(EV)-mediated targeting approach to improve in vivo tumor imaging using ternary downconversion nanoparticles(DCNPs)with strong near infrared II(NIR-II)luminescence at 1,525 nm.The EVs were metabolically engineered with azide group,followed by in vivo labeling of DCNPs through copper-free click chemistry.By taking advantage of the homologous targeting property of tumor derived EVs,remarkable improvement in the tumor accumulation(6.5%injection dose(ID)/g)was achieved in the subcutaneous colorectal cancer model when compared to that of individual DCNPs via passive targeting(1.1%ID/g).Importantly,such bioorthogonal labeling significantly increased NIR-II luminescence signals and prolonged the retention at tumor sites.Our work demonstrates the great potential of EVs-mediated bioorthogonal approach for in vivo labeling of NIR-II optical probes,which provides a robust tool for tumor-specific imaging and targeted therapy.
基金the financial support of the National Natural Science Foundation of China(52102084)Natural Science Foundation of Hunan Province(2022JJ30718)+1 种基金the financial support of the Youth Innovation Promotion Association Chinese Academy of Sciences(CAS)(2022428)the Science Fund of Shandong Laboratory of Yantai Advanced Materials and Green Manufacturing(AMGM2021A08).
文摘Synthetic zircon(ZrSiO_(4))ceramics are typically fabricated at elevated temperatures(over 1500℃),which would lead to high manufacturing cost.Meanwhile,reports about preparing ZrSiO_(4)-based ceramic composites via controlling the solid-state reaction between zirconia(ZrO_(2))and silica(SiO_(2))are limited.In this work,we proposed a low-temperature strategy to flexibly design and fabricate ZrSiO_(4)-based ceramic composites via doping and tuning the solid-state reaction.Two ceramic composites and ZrSiO_(4) ceramics were in-situ prepared by reactive fast hot pressing(FHP)at approximately 1250℃ based on the proposed strategy,i.e.,a ZrSiO_(4)-SiO_(2) dual-phase composite with bicontinuous interpenetrating and hierarchical microstructures,a ZrSiO_(4)-ZrO_(2) dual-phase composite with a microstructure of ZrO_(2) submicron-and nano-particles embedded in a micron ZrSiO_(4) matrix,and ZrSiO_(4) ceramics with a small amount of residual ZrO_(2) nanoparticles.The results showed that the phase compositions,microstructure configurations,mechanical properties,and wear resistance of the materials can be flexibly regulated by the proposed strategy.Hence,ZrSiO_(4)-based ceramic composites with different properties can be easily fabricated based on different application scenarios.These findings would offer useful guidance for researchers to flexibly fabricate ZrSiO_(4)-based ceramic composites at low temperatures and tailor their microstructures and properties through doping and tuning the solid-state reaction.
基金supported by the CSEE Youth Science & Technology Innovation Project (No.003)National Energy Applied Technology Research & Demonstration Project (No.NY20110102-1)
文摘Aging precipitation behavior and mechanical properties of Inconel 617 superalloy aged at 760℃ for up to 10000 h were investigated. The results showed that the precipitates of the aged alloy are M23C6 and M6C carbides and γ phase. The carbide particles precipitated both at the grain boundaries and within grains, and the γ phase particles were situated at intragranular sites in the process of aging. The carbide particles were discontinuously dispersed at grain boundaries after aging for 3000 h, while after aged for 5000 h the carbide particles are merged. The precipitates inside grains remained stable even after aging for 10000 h. The hardness was increased for the alloy aged for 300 h up to 3000 h, which was resulted primarily from the precipitation of carbides as discrete particles both at the grain boundaries and inside grains. Small quantity γ precipitates were formed inside grains, to some extent, which contributed to an enhanced hardness. However, a decrease of the hardness was observed after aging for 5000 h. A significant drop in toughness of the alloy aged for 300 h was attributed to the reduction of the bonding interface strength when carbides precipitated at grain boundaries. Thereafter, the toughness decreased slowly with the prolonged aging time. The high temperature tensile properties of the aged alloy are rather stable even aged for 300-3000 h.
基金supported by the National Natural Science Foundation of China (61875136 and 52002246)the Fundamental Research Project of Guangdong Province (2020A1515011315)+2 种基金Shenzhen Fundamental Research Project (JCYJ20190808170601664)the Science and Technology Innovation Project of Shenzhen Excellent Talents (RCBS20200714114919006)the Scientific Research Foundation as Phase II construction of high-level University for the Youth Scholars of Shenzhen University 2019 (000002110223)。
文摘In applications such as parallel mesh refinement,it remains a challenging issue to ensure the refined surface respects the original Computer-Aided Design(CAD)model accurately.In this paper,an ultralight geometry processing library is developed to resolve this issue effectively and efficiently.Here,we say the kernel is ultralight because it has a very small set of data-structures and algorithms by comparison with industrial-level geometry kernels.Within the library,a simplified surface boundary representation(B-rep)and a radial edge structure are developed respectively to depict the geometry model and the surface mesh,plus hash tables that record the connections between the geometry model and the surface mesh.Based on these data structures,a set of efficient algorithms are developed,which initializes the connection tables,projects a point back to the original geometry,etc.With these data-structure and algorithmic infrastructures set up,the callings of eight well-designed Application Programming Interfaces(APIs)are powerful enough to enable the parallel mesh refinement algorithm outputs a mesh respecting the input CAD model accurately.Numerical experiments will be finally presented to evaluate the performance of the overall parallel mesh refinement algorithm and the algorithms in relation with the developed library.