Au-nanocluster-loaded human serum albumin nanoparticles with enhanced cellular uptake for fluorescent imaging

Protein-directed fluorescent Au nanoclusters have been widely studied owing to their potential applications in sensing,imaging,and drug and gene delivery.However,the use of nanoclusters in drug delivery is limited by low cellular uptake.In this study,human serum albumin-directed Au nanoclusters served as building blocks to obtain protein nanoparticles by desolvation.The nanoparticles had a decent quantum yield(QY),high colloidal stability and low cytotoxicity,and they could be readily conjugated with biological molecules.The cellular uptake of the Au nanoclusters and nanocluster-loaded protein nanoparticles were studied by confocal fluorescence microscopy.Agglomeration of the protein-directed Au nanoclusters into 50–150-nm nanoparticles dramatically increased the cellular uptake.

Gold nanorods with a hematoporphyrin-loaded silica shell for dual-modality photodynamic and photothermal treatment of tumors in vivo

由一个金 nanorod 核心和与 hematoporphyrin (HP ) 做的 mesoporous 硅石壳组成的 Nanocomposites (NC ) 被制作了以便由在 vivo 联合 photothermal 和光力学的治疗(太平洋夏季时间 + PTT ) 改进癌症治疗的效率。除了 810830 nm 附近的长波长的电浆子回声，制作 NC 展出了相应的一座 400-nm 吸收度山峰围住 HP，在 632.5-nm Q 乐队附近在 633-nm 刺激下面产生了汗衫氧，并且生产了在 808-nm 下面的热在红外线附近(NIR ) 激光照耀。这些形式被用于联合太平洋夏季时间 + PTT 治疗大(大约 3 cm3 ) 在有一只 xenorafted 肿瘤老鼠的 vivo 的稳固的肿瘤当模特儿。NC 直接被注入肿瘤并且与 633-nm 和 808-nm 激光同时照耀刺激联合光力学并且 NC 的 photothermal 活动。联合治疗的效率被光连贯断层摄影术评估，组织学的分析，并且由肿瘤体积生长的大小在一个 21 天的时期期间。调停 NC 的太平洋夏季时间在组织组织学并且到导致了弱变化一中等在肿瘤体积的 20% 减少。相反，联合太平洋夏季时间 + PTT 处理导致了大区域的肿瘤坏死并且在肿瘤体积导致了戏剧的减少。