Genetic architecture of maize yield traits dissected by QTL mapping and GWAS in maize

The study of yield traits can reveal the genetic architecture of grain yield for improving maize production.In this study, an association panel comprising 362 inbred lines and a recombinant inbred line population derived from X178 × 9782 were used to identify candidate genes for nine yield traits. High-priority overlap(HPO) genes, which are genes prioritized in a genome-wide association study(GWAS), were investigated using coexpression networks. The GWAS identified 51 environmentally stable SNPs in two environments and 36 pleiotropic SNPs, including three SNPs with both attributes. Seven hotspots containing 41 trait-associated SNPs were identified on six chromosomes by permutation. Pyramiding of superior alleles showed a highly positive effect on all traits, and the phenotypic values of ear diameter and ear weight consistently corresponded with the number of superior alleles in tropical and temperate germplasm. A total of 61 HPO genes were detected after trait-associated SNPs were combined with the coexpression networks. Linkage mapping identified 16 environmentally stable and 16 pleiotropic QTL.Seven SNPs that were located in QTL intervals were assigned as consensus SNPs for the yield traits.Among the candidate genes predicted by our study, some genes were confirmed to function in seed development. The gene Zm00001 d016656 encoding a serine/threonine protein kinase was associated with five different traits across multiple environments. Some genes were uniquely expressed in specific tissues and at certain stages of seed development. These findings will provide genetic information and resources for molecular breeding of maize grain yield.

Genome-wide Association Analysis of Maize Flowering Traits

Flowering regulation is important for maize to adapt to a variety of environments as well as associated with high yield.In this study,the genetic mechanism of three flowering traits of 310 maize inbred lines with rich genetic background was investigated in three years at three different environments such as days to tasseling(DTT),days to silking(DTS)and days to pollen shedding(DTP).Based on mean performance,the longest flowering time was observed in Zhanyi(2018),whereas the shortest in Shizong(2019).The coefficient of variance depicted the range from 3.62%to 9.06%for three flowering traits under all environments.Therefore,we have integrated these flowering traits corresponding to SNP molecular markers for genome-wide association study(GWAS).Results showed that 22 SNPs markers were significantly associated with DTT according to physical position and average linkage disequilibrium(LD)decay distance,and a total of 234 candidate genes were identified near these significantly associated SNP markers.Moreover,KEGG and GO analysis showed that these genes were enriched in the regulation of the physiological pathways for flowering.In more details,16 genes involved in development of floral organs are more worthy of our attention in future studies.

Simple solutions for photonic power-efficient ultra-wideband system assisted by electrical bandpass filter

We propose and experimentally demonstrate two simple solutions for power-efficient ultra-wideband （UWB） radio frequency （RF） system assisted by an electrical bandpass filter （EBPF）. In the first solution, any optical Gaussian pulse with enough bandwidth is transmitted over optical fiber link, and then converted to a power-efficient UWB pulse by an EBPF with a passband of 3.1 10.6GHz. The transmission and modulation of UWB signal is processed in optical domain, whereas the generation of UWB is processed in electrical domain. Both UWB modulations of on-off keying （OOK） and binary phase shift keying （BPSK） are experimentally demon- strated. In the second solution, the EBPF is used to convert any electrical waveform to a power-efficient UWB pulse. Then the electrical UWB pulse is converted to an optical UWB pulse with a Mach-Zehnder modulator （MZM）, and then distributed over long haul fiber link. These two solutions embody the advantages of both low-loss long- haul transmission of optical fiber and mature electrical circuits. And the millimeter-wave UWB signal is also demonstrated.