To date, the coronavirus disease 2019 (COVID-19) outbreak has become a global pandemic and public health disaster. In addition to acute respiratory manifestations, patients with COVID-19 exhibit other non-respiratory ...To date, the coronavirus disease 2019 (COVID-19) outbreak has become a global pandemic and public health disaster. In addition to acute respiratory manifestations, patients with COVID-19 exhibit other non-respiratory manifestations, particularly in those with severe underlying disease. Few specific therapeutics are effective for COVID-19, and supportive care is the primary remedy. Here, we comprehensively surveyed the most recent reports on extrapulmonary complications of COVID-19 and their corresponding treatments, as well as the comparison of different clinical symptoms and complications of COVID-19, severe acute respiratory syndrome (SARS), and the Middle East respiratory syndrome (MERS) patients. We wish to provide a molecular and cellular understanding of the complications of COVID-19 and provide guidance for future diagnostics, therapeutics, and prognostics of COVID-19.展开更多
Sonography for non-invasive and continuous cardiac function imaging and measurement is essential for the diagnosis and prognosis of cardiovascular diseases[1].The combination of electronic device miniaturization and f...Sonography for non-invasive and continuous cardiac function imaging and measurement is essential for the diagnosis and prognosis of cardiovascular diseases[1].The combination of electronic device miniaturization and flexible manufacturing technology has resulted in great progress in the development of wearable ultrasound patches and probes in recent years and has presented numerous alternative strategies for current clinically available technologies[2,3].展开更多
Sweat loss monitoring is important for understanding the body’s thermoregulation and hydration status,as well as for comprehensive sweat analysis.Despite recent advances,developing a low-cost,scalable,and universal m...Sweat loss monitoring is important for understanding the body’s thermoregulation and hydration status,as well as for comprehensive sweat analysis.Despite recent advances,developing a low-cost,scalable,and universal method for the fabrication of colorimetric microfluidics designed for sweat loss monitoring remains challenging.In this study,we propose a novel laserengraved surface roughening strategy for various flexible substrates.This process permits the construction of microchannels that show distinct structural reflectance changes before and after sweat filling.By leveraging these unique optical properties,we have developed a fully laser-engraved microfluidic device for the quantification of naked-eye sweat loss.This sweat loss sensor is capable of a volume resolution of 0.5µL and a total volume capacity of 11µL,and can be customized to meet different performance requirements.Moreover,we report the development of a crosstalk-free dual-mode sweat microfluidic system that integrates an Ag/AgCl chloride sensor and a matching wireless measurement flexible printed circuit board.This integrated system enables the real-time monitoring of colorimetric sweat loss signals and potential ion concentration signals without crosstalk.Finally,we demonstrate the potential practical use of this microfluidic sweat loss sensor and its integrated system for sports medicine via on-body studies.展开更多
Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AI...Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AIE materials is to systematically and vigorously evaluate their biosafety and biocompatibility.While previous studies focus on cellular viability and toxicity,the impact of AIE materials on detailed stress responses manifesting cellular fitness has been less explored.Herein,this work provides the first piece of evidence to support amphiphilic functionalization of AIE nanoparticles minimizes the deterioration on proteome stability and cellular protein homeostasis(proteostasis).To this end,four scaffolds of AIE molecules were prepared,further functionalized into eight nanoparticles with two amphiphilic shells respectively,and characterized for their physicochemical properties.Thermal shift assay quantitatively demonstrates that AIE materials after amphiphilic functionalization into nanoparticles enhance proteome thermodynamic stability and ameliorate proteome aggregation propensity in cellular lysate,echoed by cell viability and fractionation experiments.Intriguingly,poor polydispersity index(PDI)of functionalized nanoparticles exaggerates their retention and aggregation in the cell.Comparative proteomic analysis uncovers that amphiphilic functionalization of AIE materials can minimize the deterioration of cellular protein homeostasis network.Finally,vigorous interrogation of functionalized AIE nanoparticles in mice model reveals the complexity of factors affecting the biocompatibility profiles in vivo,including materials’size,PDI,and treatment frequencies.Overall,amphiphilic functionalization of AIE materials into nanoparticles is necessary to maintain proteome stability and balance cellular protein homeostasis.展开更多
文摘To date, the coronavirus disease 2019 (COVID-19) outbreak has become a global pandemic and public health disaster. In addition to acute respiratory manifestations, patients with COVID-19 exhibit other non-respiratory manifestations, particularly in those with severe underlying disease. Few specific therapeutics are effective for COVID-19, and supportive care is the primary remedy. Here, we comprehensively surveyed the most recent reports on extrapulmonary complications of COVID-19 and their corresponding treatments, as well as the comparison of different clinical symptoms and complications of COVID-19, severe acute respiratory syndrome (SARS), and the Middle East respiratory syndrome (MERS) patients. We wish to provide a molecular and cellular understanding of the complications of COVID-19 and provide guidance for future diagnostics, therapeutics, and prognostics of COVID-19.
基金supported by the National Natural Science Foundation of China (62174152)。
文摘Sonography for non-invasive and continuous cardiac function imaging and measurement is essential for the diagnosis and prognosis of cardiovascular diseases[1].The combination of electronic device miniaturization and flexible manufacturing technology has resulted in great progress in the development of wearable ultrasound patches and probes in recent years and has presented numerous alternative strategies for current clinically available technologies[2,3].
基金support from the National Natural Science Foundation of China(No.62174152)。
文摘Sweat loss monitoring is important for understanding the body’s thermoregulation and hydration status,as well as for comprehensive sweat analysis.Despite recent advances,developing a low-cost,scalable,and universal method for the fabrication of colorimetric microfluidics designed for sweat loss monitoring remains challenging.In this study,we propose a novel laserengraved surface roughening strategy for various flexible substrates.This process permits the construction of microchannels that show distinct structural reflectance changes before and after sweat filling.By leveraging these unique optical properties,we have developed a fully laser-engraved microfluidic device for the quantification of naked-eye sweat loss.This sweat loss sensor is capable of a volume resolution of 0.5µL and a total volume capacity of 11µL,and can be customized to meet different performance requirements.Moreover,we report the development of a crosstalk-free dual-mode sweat microfluidic system that integrates an Ag/AgCl chloride sensor and a matching wireless measurement flexible printed circuit board.This integrated system enables the real-time monitoring of colorimetric sweat loss signals and potential ion concentration signals without crosstalk.Finally,we demonstrate the potential practical use of this microfluidic sweat loss sensor and its integrated system for sports medicine via on-body studies.
基金National Natural Science Foundation of China,Grant/Award Numbers:22107100,21907091,21725506China Postdoctoral Science Foundation,Grant/Award Number:2019M661138+2 种基金LiaoNing Revitalization Talents Program from the Liaoning province of China,Grant/Award Number:XLYC1907048Dalian Innovation Fund,Grant/Award Number:2020JJ26GX027Youth Innovation Promotion Association,CAS,Grant/Award Number:2020184。
文摘Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AIE materials is to systematically and vigorously evaluate their biosafety and biocompatibility.While previous studies focus on cellular viability and toxicity,the impact of AIE materials on detailed stress responses manifesting cellular fitness has been less explored.Herein,this work provides the first piece of evidence to support amphiphilic functionalization of AIE nanoparticles minimizes the deterioration on proteome stability and cellular protein homeostasis(proteostasis).To this end,four scaffolds of AIE molecules were prepared,further functionalized into eight nanoparticles with two amphiphilic shells respectively,and characterized for their physicochemical properties.Thermal shift assay quantitatively demonstrates that AIE materials after amphiphilic functionalization into nanoparticles enhance proteome thermodynamic stability and ameliorate proteome aggregation propensity in cellular lysate,echoed by cell viability and fractionation experiments.Intriguingly,poor polydispersity index(PDI)of functionalized nanoparticles exaggerates their retention and aggregation in the cell.Comparative proteomic analysis uncovers that amphiphilic functionalization of AIE materials can minimize the deterioration of cellular protein homeostasis network.Finally,vigorous interrogation of functionalized AIE nanoparticles in mice model reveals the complexity of factors affecting the biocompatibility profiles in vivo,including materials’size,PDI,and treatment frequencies.Overall,amphiphilic functionalization of AIE materials into nanoparticles is necessary to maintain proteome stability and balance cellular protein homeostasis.