High levels of cell-free DNA(cfDNA)induce psoriasis.Currently,the treatment of psoriasis has the disadvantages of penetration difficulty,suppression of normal immunity,and skin irritation.In this study,biguanide chito...High levels of cell-free DNA(cfDNA)induce psoriasis.Currently,the treatment of psoriasis has the disadvantages of penetration difficulty,suppression of normal immunity,and skin irritation.In this study,biguanide chitosan microneedles(BGC-MNs)were prepared to treat psoriasis by removing cfDNA from the dermis through the skin barrier.The effects of chitosan with different bisguanidine contents on DNA-binding capacity,biocompatibility,and inflammation inhibition were compared,revealing that chitosan containing 20%bisguanidine(BGC2)was found to have the best overall performance.In vitro,BGC2 effectively cleared cfDNA and inhibited the production of inflammatory factors.BGC-MN made from BGC2 had good mechanical and solubility properties.In vivo,BGC-MNs cleared cfDNA,reduced the level of inflammatory factors in the dermis,and exerted a good therapeutic effect on mice with psoriasis.These results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.展开更多
基金supported by National Key R&D Program of China(Grant number 2021YFB3800900)National Natural Science Foundation of China(Grant numbers 52073013,22122501,52221006 and 52161145410)+1 种基金Beijing Outstanding Young Scientist Program(No.BJJWZYJH01201910010024)approved by the Ethical Committee of Chinese Academy of Medical Sciences(CAMS).
文摘High levels of cell-free DNA(cfDNA)induce psoriasis.Currently,the treatment of psoriasis has the disadvantages of penetration difficulty,suppression of normal immunity,and skin irritation.In this study,biguanide chitosan microneedles(BGC-MNs)were prepared to treat psoriasis by removing cfDNA from the dermis through the skin barrier.The effects of chitosan with different bisguanidine contents on DNA-binding capacity,biocompatibility,and inflammation inhibition were compared,revealing that chitosan containing 20%bisguanidine(BGC2)was found to have the best overall performance.In vitro,BGC2 effectively cleared cfDNA and inhibited the production of inflammatory factors.BGC-MN made from BGC2 had good mechanical and solubility properties.In vivo,BGC-MNs cleared cfDNA,reduced the level of inflammatory factors in the dermis,and exerted a good therapeutic effect on mice with psoriasis.These results suggested that BGC-MNs provided a new approach to treating psoriasis in terms of scavenging cfDNA and exerting anti-inflammatory effects.
