Objective: To study the vitronectin expression in human spermato-zoa and its role in fertilization. Methods: Spermatozoa from 14 fer-tile and 8 infertile men with normal semen data were studied. Follow-ing recovery of...Objective: To study the vitronectin expression in human spermato-zoa and its role in fertilization. Methods: Spermatozoa from 14 fer-tile and 8 infertile men with normal semen data were studied. Follow-ing recovery of motile populations by swim-up, spermatozoa were ca-pacitated and immunostained with rabbit anti-human Vn polyclonal an-tibody and goat anti-rabbit IgG-FTTC second antibody. The percentageof spermatozoa expressing Vn was determined using a FAScan flowcytometer. Meanwhile, the fertilizing ability of capacitated spermato-zoa was determined with human spermatozoa zona-free hamster eggpenetration assay (SPA). Results: The mean ± s proportion ofspermatozoa expressing Vn of fertile men was 21.24% ± 11.70% and3.64±3.27% for infertile men (P<0.05). The penetration rate ofSPA in the fertile group was > 10%, but that in the infertile group ,< 10%. There is a correlation between positive sperm Vn expressionand percentage of eggs penetrated (r=0.476). Conclusion: Theseresults indicate the expression of Vn in human capacitated spermatozoaand its correlation with the fertilizing ability. The abnormal expressionof Vn on human spermatozoa may be one of the unexplained infertilereasons. (Reprod Contracep 2001; 21: 24-28)展开更多
文摘Objective: To study the vitronectin expression in human spermato-zoa and its role in fertilization. Methods: Spermatozoa from 14 fer-tile and 8 infertile men with normal semen data were studied. Follow-ing recovery of motile populations by swim-up, spermatozoa were ca-pacitated and immunostained with rabbit anti-human Vn polyclonal an-tibody and goat anti-rabbit IgG-FTTC second antibody. The percentageof spermatozoa expressing Vn was determined using a FAScan flowcytometer. Meanwhile, the fertilizing ability of capacitated spermato-zoa was determined with human spermatozoa zona-free hamster eggpenetration assay (SPA). Results: The mean ± s proportion ofspermatozoa expressing Vn of fertile men was 21.24% ± 11.70% and3.64±3.27% for infertile men (P<0.05). The penetration rate ofSPA in the fertile group was > 10%, but that in the infertile group ,< 10%. There is a correlation between positive sperm Vn expressionand percentage of eggs penetrated (r=0.476). Conclusion: Theseresults indicate the expression of Vn in human capacitated spermatozoaand its correlation with the fertilizing ability. The abnormal expressionof Vn on human spermatozoa may be one of the unexplained infertilereasons. (Reprod Contracep 2001; 21: 24-28)
