The fish Prochilodus lineatus (Characiformes, Prochilodontidae), in addition to being a good bioindicator, is also of economic and ecological importance with a broad distribution in the neotropics. Ecotoxicology exami...The fish Prochilodus lineatus (Characiformes, Prochilodontidae), in addition to being a good bioindicator, is also of economic and ecological importance with a broad distribution in the neotropics. Ecotoxicology examines the interaction between environmental chemistry and biota;and in this study we assess alterations of bile and glycogen levels in the fish liver, organ responsible for detoxification, biotransformation and storing nutrients, such as glycogen, and for secreting bile. Fish were separated in three groups to examine the damage caused by the exposure to waters from Lago Azul-Rio Claro-SP and containing diluted biodegradable detergents in comparison to a control group (chlorinated water from an artesian well of UNESP-Campus Rio Claro). A histological analysis was performed on HE and PAS stained sections. The identification of structural changes and the assessment of the area occupied by bile and glycogen were carried out with the software ImageJ, showing that the liver was affected morphologically (cell vacuolization, peripherals nuclei, for example) and problems in bile release and production and storage of glycogen.展开更多
Baccharis dracunculifolia, popularly known in Brazil as “alecrim-do-campo”, is widely recognized for its therapeutic potential. The extract of its leaves is used for liver problems, stomach disorders and others. The...Baccharis dracunculifolia, popularly known in Brazil as “alecrim-do-campo”, is widely recognized for its therapeutic potential. The extract of its leaves is used for liver problems, stomach disorders and others. The objective of the present study was to perform a histochemical analysis of curimbata fish livers to evaluate the potential effects and risks of the ingestion of B. dracunculifolia. Thirty-two animals were divided into two experimental groups in duplicate: Control group (regular food) and B. dracunculifolia Treated group (food added with B. dracunculifolia). The fishes were collected on the 14th and 21st days after the treatment period of 21 days. The histological alterations were evaluated using the semiquantitative methods Mean Value of Alterations (MVA), Histopathological Alteration Index (HAI) and Image J®. HAI and MAV showed that the extract caused slight but statistically significant damages, widely distributed throughout the organ. The results showed significant hepatic alterations caused by the ingestion of B. dracunculifolia extract.展开更多
Indiscriminate disposal of residues of domestic and/or industrial origin in aquatic ecosystems resulting from urban activities causes concern about their effects on the fauna. Substances absorbed by animals, such as t...Indiscriminate disposal of residues of domestic and/or industrial origin in aquatic ecosystems resulting from urban activities causes concern about their effects on the fauna. Substances absorbed by animals, such as those cited above, are metabolized by the liver, as it is the main target organ of several pollutants and also exercises basic vital functions in the organism. For this reason the liver is used as the main and most reliable biomarker in environmental studies. Thus the objective of the present study was to identify hepatic alterations resulting from exposure to water contaminated by a few commercial brands of biodegradable detergents and their effects when present together with several other contaminants found in nature. For such, a solution of 1 ppm of biodegradable detergents and water from an urban lake were tested on two widely distributed native Brazilian fish (Astyanax altiparanae and Prochilodus lineatus). Several alterations were found on the liver, including cytoplasm vacuolization, nuclear displacement, macrophage recruitment and reduced liver glycogen, showing a degenerative process in the liver of the fish, due to the action of contaminants.展开更多
Fish populations are sensitive to environmental impact resulting from many factors, as an introduction of exotic species, industrial or residual waste, oil spills, pesticides or other agents that can directly affect t...Fish populations are sensitive to environmental impact resulting from many factors, as an introduction of exotic species, industrial or residual waste, oil spills, pesticides or other agents that can directly affect the ecology and species survival. Astyanax altiparanae is pelagic and active swimmer specie and can have serious implications on its way of life as changes in muscle structure of fish may occur since the presence of pollutants in the water. In this study we analyze the alterations in Muscle and collagen fibers of the pectoral muscles and pollutants exposed flows contained in water with biodegradable detergents, and water coming from the Blue Lake, River clear-SP (2o24'39"S, 47o33'39"E) diluted. Effects of these exposures were analyzed during the period of seven and thirty days. Compared with acclimatized fish (Astyanax altiparanae) with pure water from an artesian well, the muscle fibers morphology and collagen concentration on them have changed due to the exposure to urban pollutants and biodegradable detergents.展开更多
文摘The fish Prochilodus lineatus (Characiformes, Prochilodontidae), in addition to being a good bioindicator, is also of economic and ecological importance with a broad distribution in the neotropics. Ecotoxicology examines the interaction between environmental chemistry and biota;and in this study we assess alterations of bile and glycogen levels in the fish liver, organ responsible for detoxification, biotransformation and storing nutrients, such as glycogen, and for secreting bile. Fish were separated in three groups to examine the damage caused by the exposure to waters from Lago Azul-Rio Claro-SP and containing diluted biodegradable detergents in comparison to a control group (chlorinated water from an artesian well of UNESP-Campus Rio Claro). A histological analysis was performed on HE and PAS stained sections. The identification of structural changes and the assessment of the area occupied by bile and glycogen were carried out with the software ImageJ, showing that the liver was affected morphologically (cell vacuolization, peripherals nuclei, for example) and problems in bile release and production and storage of glycogen.
基金the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)support
文摘Baccharis dracunculifolia, popularly known in Brazil as “alecrim-do-campo”, is widely recognized for its therapeutic potential. The extract of its leaves is used for liver problems, stomach disorders and others. The objective of the present study was to perform a histochemical analysis of curimbata fish livers to evaluate the potential effects and risks of the ingestion of B. dracunculifolia. Thirty-two animals were divided into two experimental groups in duplicate: Control group (regular food) and B. dracunculifolia Treated group (food added with B. dracunculifolia). The fishes were collected on the 14th and 21st days after the treatment period of 21 days. The histological alterations were evaluated using the semiquantitative methods Mean Value of Alterations (MVA), Histopathological Alteration Index (HAI) and Image J®. HAI and MAV showed that the extract caused slight but statistically significant damages, widely distributed throughout the organ. The results showed significant hepatic alterations caused by the ingestion of B. dracunculifolia extract.
基金FAPESP process number:2009/17118-9 for financial support
文摘Indiscriminate disposal of residues of domestic and/or industrial origin in aquatic ecosystems resulting from urban activities causes concern about their effects on the fauna. Substances absorbed by animals, such as those cited above, are metabolized by the liver, as it is the main target organ of several pollutants and also exercises basic vital functions in the organism. For this reason the liver is used as the main and most reliable biomarker in environmental studies. Thus the objective of the present study was to identify hepatic alterations resulting from exposure to water contaminated by a few commercial brands of biodegradable detergents and their effects when present together with several other contaminants found in nature. For such, a solution of 1 ppm of biodegradable detergents and water from an urban lake were tested on two widely distributed native Brazilian fish (Astyanax altiparanae and Prochilodus lineatus). Several alterations were found on the liver, including cytoplasm vacuolization, nuclear displacement, macrophage recruitment and reduced liver glycogen, showing a degenerative process in the liver of the fish, due to the action of contaminants.
文摘Fish populations are sensitive to environmental impact resulting from many factors, as an introduction of exotic species, industrial or residual waste, oil spills, pesticides or other agents that can directly affect the ecology and species survival. Astyanax altiparanae is pelagic and active swimmer specie and can have serious implications on its way of life as changes in muscle structure of fish may occur since the presence of pollutants in the water. In this study we analyze the alterations in Muscle and collagen fibers of the pectoral muscles and pollutants exposed flows contained in water with biodegradable detergents, and water coming from the Blue Lake, River clear-SP (2o24'39"S, 47o33'39"E) diluted. Effects of these exposures were analyzed during the period of seven and thirty days. Compared with acclimatized fish (Astyanax altiparanae) with pure water from an artesian well, the muscle fibers morphology and collagen concentration on them have changed due to the exposure to urban pollutants and biodegradable detergents.
