AIM: To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer.METHODS: Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measu...AIM: To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer.METHODS: Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measured by electron gastroscope before and after treatment, then the inhibitory and effective rates were calculated. The ultrastructures of tumor cells were examined by transmissional electron microscope. Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of human gastric cancer SGC-7901 cells.RESULTS: Compared with the statement before treatment,GBEP capsules could reduce the area of tumors, and the effective rate was 73.4 %. Ultrastructural changes of the cells indicated that GBEP could induce apoptosis and differentiation in tumor cells of patients with gastric cancer.GBEP could inhibit the growth of human gastric cancer SGC7901 cells following 24-72 h treatment in vitro at 10-320 mg/L,which was dose- and time-dependent. GBEP was able to elevate the apoptosis rate and expression of c-los gene,but reduce the expression of c-myc and bcl-2 genes also in a dose-dependent manner.CONCLUSION: The therapeutic mechanism of GBEP on human gastric cancer may relate to its effects on the expression of c-myc, bcl-2 and c-los genes, which can inhibit proliferation and induce apoptosis and differentiation of tumor cells.展开更多
基金the Society Development Foundation of Jiangsu Province,No.BS 2000086
文摘AIM: To study the therapeutic mechanism of Ginkgo biloba exocarp polysaccharides (GBEP) on gastric cancer.METHODS: Thirty patients with gastric cancer were treated with oral GBEP capsules. The area of tumors was measured by electron gastroscope before and after treatment, then the inhibitory and effective rates were calculated. The ultrastructures of tumor cells were examined by transmissional electron microscope. Cell culture, MTT, flow cytometry were performed to observe proliferation, apoptosis and changes of relevant gene expression of human gastric cancer SGC-7901 cells.RESULTS: Compared with the statement before treatment,GBEP capsules could reduce the area of tumors, and the effective rate was 73.4 %. Ultrastructural changes of the cells indicated that GBEP could induce apoptosis and differentiation in tumor cells of patients with gastric cancer.GBEP could inhibit the growth of human gastric cancer SGC7901 cells following 24-72 h treatment in vitro at 10-320 mg/L,which was dose- and time-dependent. GBEP was able to elevate the apoptosis rate and expression of c-los gene,but reduce the expression of c-myc and bcl-2 genes also in a dose-dependent manner.CONCLUSION: The therapeutic mechanism of GBEP on human gastric cancer may relate to its effects on the expression of c-myc, bcl-2 and c-los genes, which can inhibit proliferation and induce apoptosis and differentiation of tumor cells.
