In the present study,Pickering nanoemulsions(oil-in-water)co-stabilized with whey protein isolate(WPI)and three types of small molecular surfactants were prepared.Hexadecyltrimethylammonium(CTAB),sodium dodecyl sulfat...In the present study,Pickering nanoemulsions(oil-in-water)co-stabilized with whey protein isolate(WPI)and three types of small molecular surfactants were prepared.Hexadecyltrimethylammonium(CTAB),sodium dodecyl sulfate(SDS),and Tween 20 were used as cationic,anionic,and nonionic surfactants,respectively.To investigate effects of small molecular surfactants on physical stability,turbidimetric,and rheological properties of protein-stabilized Pickering nanoemulsions,various treatments mimicking food processing including pH,heat,freeze-thaw,and salt were applied.Results revealed that the droplet size and polydispersity index of the nanoemulsion prepared with WPI alone were increased after heat treatment or at pH condition near the isoelectric point of WPI,indicating a low physical stability.However,nanoemulsions prepared with WPI-SDS or WPI-CTAB maintained their initial state even after heat treatment including autoclave.Furthermore,there was no significant change in the droplet size of the nanoemulsion prepared with WPI-Tween after freeze-thaw and salt treatments.Although Pickering nanoemulsion stabilized with both WPI and SDS had the highest viscosity,Turbiscan stability index(TSI)was the highest.However,the TSI of the nanoemulsions added with CTAB or Tween 20 was lower than that of the nanoemulsions prepared with WPI alone,confirming that the storage stability of protein-stabilized Pickering nanoemulsion was improved by adding small molecular surfactants.展开更多
Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble...Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble protein(WPB)and salt-soluble protein(SPB)extracts derived from Protaetia brevitarsis against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in C2C12 cells were investigated.WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities.In addition,WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment,and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro-and anti-apoptotic proteins.Therefore,our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.展开更多
基金supported by a grant from Kyung Hee University in 2022(KHU-20220906)by a grant from Korea Food Research Institute(E0211200-02).
文摘In the present study,Pickering nanoemulsions(oil-in-water)co-stabilized with whey protein isolate(WPI)and three types of small molecular surfactants were prepared.Hexadecyltrimethylammonium(CTAB),sodium dodecyl sulfate(SDS),and Tween 20 were used as cationic,anionic,and nonionic surfactants,respectively.To investigate effects of small molecular surfactants on physical stability,turbidimetric,and rheological properties of protein-stabilized Pickering nanoemulsions,various treatments mimicking food processing including pH,heat,freeze-thaw,and salt were applied.Results revealed that the droplet size and polydispersity index of the nanoemulsion prepared with WPI alone were increased after heat treatment or at pH condition near the isoelectric point of WPI,indicating a low physical stability.However,nanoemulsions prepared with WPI-SDS or WPI-CTAB maintained their initial state even after heat treatment including autoclave.Furthermore,there was no significant change in the droplet size of the nanoemulsion prepared with WPI-Tween after freeze-thaw and salt treatments.Although Pickering nanoemulsion stabilized with both WPI and SDS had the highest viscosity,Turbiscan stability index(TSI)was the highest.However,the TSI of the nanoemulsions added with CTAB or Tween 20 was lower than that of the nanoemulsions prepared with WPI alone,confirming that the storage stability of protein-stabilized Pickering nanoemulsion was improved by adding small molecular surfactants.
基金This research was supported by the Main Research Program(E0211200-03)of the Korea Food Research Institute(KFRI)funded by the Ministry of Science and ICT(Republic of Korea).
文摘Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional activity.In this study,the apoptotic protective effects of water-soluble protein(WPB)and salt-soluble protein(SPB)extracts derived from Protaetia brevitarsis against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in C2C12 cells were investigated.WPB and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing activities.In addition,WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment,and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro-and anti-apoptotic proteins.Therefore,our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.
