犬细小病毒吉林流行株的分离鉴定及VP2基因的遗传进化分析

从吉林省长春市某动物医院采集了3份CPV阳性宠物犬粪便样品,经PCR鉴定,使用F81细胞进行盲传,同时对病毒进行血凝、电镜观察及间接免疫荧光等检测后,对其VP2基因进行序列分析。结果显示,3株CPV分离株为CPV-2c型犬细小病毒(命名为CPV-JL21-L1、CPV-JL21-L2、CPV-JL21-L3),第20代病毒滴度均在10^(-7)~10^(-7.5)TCID50/0.1 mL之间,第20代血凝效价结果均保持在210~211。VP2基因序列遗传进化分析显示,3株CPV分离株与3株疫苗株VP2基因的同源性为98.6%~99.0%,与其他参考毒株同源性为98.6%~99.9%。此外,分离株CPV-JL21-L1和CPV-JL21-L3在80 L、101 T、267 Y、297 A、300 G、324 I、375 D、426 E和555 V位点与国内流行株相同,CPV-JL21-L2在267位点氨基酸发生了变异。本研究为吉林省CPV分子流行病学的调查提供理论依据。

论龙舟运动的课程化教学研究

作为中华民族优秀传统体育项目之一——龙舟运动,至今已经走过两千多年的岁月。随着龙舟运动项目在我国竞技场上的不断壮大,而后在我国高校中也迅速广泛开展开来。而这也就标志着龙舟运动项目的传播和发展已经从传统的民间活动形式逐步转化为现代高校的课程化教学模式,当前龙舟运动项目的课程化教学已经远远落后于当前的龙舟现状,龙舟体育项目的课程化教学研究面临的是日益进步的科技文化知识理论的冲击,需要在不断研究和实践中以科学性、整体性的体系对其不断完善,从而使龙舟体育项目在我国高校的传播和发展中不断成长和壮大。本文旨在结合龙舟体育运动的特性,运用高校教育学相关课程和教学的基本原理,探索龙舟体育课程的理论框架、实践内容、学科体系和保障策略等问题,对我国高校龙舟运动项目的课程化教学进行讨论研究。

Summertime temperature variations in the middle and lower reaches of Yangtze River and their related circulation anomalies in the past five decades

Using the daily data of temperature from China Meteorological Administration and the NCEP/NCAR reanalysis from 1960 to 2005, we have analyzed the relationships between the summertime high/low temperature events in the middle and lower reaches of the Yangtze River （MLRYR） and the related circulation anomalies in the Eastern Hemisphere. Our results have demonstrated that a significantly increasing trend is observed in daily minimum temperature in the past 50 years. And in some regions in the Northern Hemisphere, the opposite scenarios are observed in circulation anomalies in lower and upper parts of the troposphere in the years when the temperatures are higher than normal, as compared to those in the years when the temperatures are lower than normal in the middle and lower reaches of the Yangtze River （MLRYR）. Additionally, the anomalous circulation structure in vertical direction in both the high and lower temperature years are barotropic. It is found that the emergence and maintenance of the aforementioned anomalous circulations are related to three kinds of wave train teleconnection patterns. Further more, influences of the long wave surface radiation on the air temperature are stronger in the nighttime than that in the daytime. While both the maximum and minimum temperatures have negative relationships with the sensible heat flux but positive relationships with the latent heat flux. To some extent, the anomalous dynamic heating （cooling） caused by the vertical thermal advection as well as the diabatic heating （cooling） caused by diabatic processes can explain the formation of the high （low） temperature events in the middle and lower reaches of the Yangtze River （MLRYR） in boreal summer.

化学发光法臭氧在线分析仪研制及应用

针对大气臭氧高精度监测需求,自主研制了基于化学发光法技术的大气臭氧在线分析仪。性能测试结果显示:该分析仪检出限≤0.5ppb,在0~500ppb内线性相关性R2接近于1.0,重复性≤1%,且7天的零点漂移与量程漂移均较小,能满足大气臭氧在线监测需求。在VOC干扰测试中,该仪器对TO15与PAMS基本无响应,而紫外吸收法臭氧分析仪对TO15与PAMS存在1倍以上的响应。此外,与API T265进行了1个月室外比对测试,两者测试结果一致性较好,相关系数R2达到0.9939,进一步表明该仪器具有较好的运行稳定性和准确性,适用于大气臭氧高精度在线监测领域。

基于CFD的城市轨道交通车厢空气质量模拟分析

城市轨道交通车厢的人员密度较高,车厢内的空气质量对乘坐体验及健康具有显著影响。以城市轨道交通列车车厢为例,采用CFD软件Fluent2019对正常载客情况下轨交车厢内空气质量进行数值模拟,分析空调送风风速为2、2.5、3 m/s和送风角度30°、45°、90°的温度场、速度场、污染物浓度场情况,提出在常态化防疫背景下,保证车厢最佳的空气流通率的通风方式为上送下回,送风速度为3 m/s、送风角度为垂直90°。

Possible Combined Influences of Absorbing Aerosols and Anomalous Atmospheric Circulation on Summertime Diurnal Temperature Range Variation over the Middle and Lower Reaches of the Yangtze River

Based on the temperature data from the China Meteorological Administration, NCEP-NCAR reanalysis data, and the TOMS Aerosol Index （AI）, we analyze the variations in the summertime diurnal temperature range （DTR） and temperature maxima in the middle and lower reaches of the Yangtze River （MLRYR） in China. The possible relationships between the direct warming effect of the absorbing aerosol and temperature variations are further investigated, although with some uncertainties. It is found that the summertime DTR exhibits a decreasing trend over the most recent 50 years, along with a slight increasing tendency since the 1980s. The trend of the maximum temperature is in agreement with those of the DTR and the absorbing aerosols. To investigate the causes of the large anomalies in the temperature maxima, composite analyses of the circulation anomalies are performed. When anomalous AI and anomalous maximum temperature over the MLRYR have the same sign, an anomalous circulation with a quasi-barotropic structure occurs there. This anomalous circulation is modulated by the Rossby wave energy propagations from the regions northwest of the MLRYR and influences the northwestern Pacific subtropical high over the MLRYR. In combination with aerosols, the anomalous circulation may increase the maximum temperature in this region. Conversely, when the anomalous AI and anomalous maximum temperature in the MLRYR have opposite signs, the anomalous circulation is not equivalently barotropic, which possibly offsets the warming effect of aerosols on the maximum temperature changes in this region. These results are helpful for a better understanding of the DTR changes and the occurrences of temperature extremes in the MLRYR region during boreal summer.

法氏囊活性肽BP7和BP9调节T细胞的功能研究

旨在探索法氏囊活性肽BP7和BP9调节T细胞亚型的功能。采用禽流感灭活疫苗免疫小鼠,利用磁珠CD3阴选分选免疫小鼠T细胞,再用法氏囊多肽BP7和BP9刺激T细胞,检测T细胞活力及亚型变化情况。MTT结果表明,BP7和BP9均能提高活化T细胞的活力水平;流式分析图显示,法氏囊多肽BP7促进活化T细胞增殖分化成CD3^(+)CD4^(+)T细胞和CD3^(+)CD8^(+) T细胞,而BP9在48 h促进CD3^(+)CD8^(+) T细胞比例增加,但未达到差异水平;细胞凋亡结果显示,BP7和BP9均能抑制T淋巴细胞早期凋亡。此外,ELISA检测表明,BP7促进T细胞分泌IL-4和IFN-γ。以上结果表明:法氏囊活性肽BP7和BP9均参与T细胞分化成熟反应,但其调节功能略有差异。

H9N2亚型禽流感病毒NA和M2基因的克隆与表达

旨在分别构建NA蛋白和M2蛋白的重组质粒,并进行表达鉴定。采用PCR扩增了H9N2分离株A/chicken/Shandong/LY1/2017毒株的NA基因和M2基因。同源性分析证实,这2个基因序列与目前流行毒株的基因同源性很高。将NA基因克隆至pcold1原核表达载体,将M2基因克隆至pET28a原核表达载体,成功构建了pcold1-NA和pET28a-M2重组质粒,并成功表达出相应的融合蛋白。然后对表达的融合蛋白进行纯化,并采用免疫印迹验证其免疫反应性。本试验为进一步研究H9N2亚型禽流感病毒致病机制及检测技术奠定了基础。

CBL蛋白表达对H9N2亚型禽流感病毒细胞中复制及感染细胞凋亡的影响

CBL蛋白是一种E3泛素连接酶,在免疫调控、信号传导调节和对多种刺激的反应中起重要作用。本研究的主要目的是探讨真核表达的CBL蛋白对H9N2禽流感病毒增殖及感染细胞的凋亡情况的影响。荧光显微镜下观察真核表达重组载体EGFP-CBL在MDCK细胞内正常表达,qPCR结果证实禽流感病毒H9N2亚型感染的转染EGFP-CBL重组载体的MDCK细胞内病毒滴度明显少;Western blot结果证实禽流感病毒H9N2亚型感染的转染EGFP-CBL重组载体的MDCK细胞36 h,其凋亡蛋白Bcl-2/Bax比值较大;流式分析结果显示转染EGFP-CBL重组载体的MDCK的早期凋亡细胞的比例少,暗示CBL蛋白能够一定程度上抑制MDCK细胞的凋亡。该结果表明CBL蛋白能在一定程度上抑制H9N2禽流感病毒在MDCK细胞的扩增,并且抑制细胞的凋亡,为深入研究CBL蛋白抗病毒细胞机制提供了重要的试验依据。