为了对发酵桑叶茶工艺条件进行优化并探究桑叶茶经发酵后抗氧化活性的变化,该试验通过响应面分析法确定发酵桑叶茶在发酵温度、发酵时间和黑曲霉接种量3种变量条件下的最佳工艺条件,并测定发酵桑叶茶生物活性物质含量及其抗氧化性。结...为了对发酵桑叶茶工艺条件进行优化并探究桑叶茶经发酵后抗氧化活性的变化,该试验通过响应面分析法确定发酵桑叶茶在发酵温度、发酵时间和黑曲霉接种量3种变量条件下的最佳工艺条件,并测定发酵桑叶茶生物活性物质含量及其抗氧化性。结果表明,最佳工艺条件为发酵时间5 d 16 h,发酵温度30℃,接种量16.55%,此条件下所制发酵桑叶茶感官评分高达90分,且其总黄酮、总多糖、多酚、游离氨基酸、芦丁、槲皮素含量比桑叶绿茶分别升高了14.39%、12.67%、2.90%、3.46%、81.13%、19.35%,还检测到了含量7.55%±0.37%的茶褐素。此外,1/1000(g/mL)浓度下发酵桑叶茶对羟基自由基、DPPH自由基、超氧自由基的清除率和对二价铁离子的螯合率分别是桑叶绿茶的1.16倍、1.07倍、1.06倍和0.82倍,该研究结果为今后桑叶的进一步开发与应用提供理论基础和参考。展开更多
Type I interferon(IFN)is considered as a bridge between innate and adaptive immunity.Proper activation or inhibition of type I IFN signaling is essential for host defense against pathogen invasion,tumor cell prolifera...Type I interferon(IFN)is considered as a bridge between innate and adaptive immunity.Proper activation or inhibition of type I IFN signaling is essential for host defense against pathogen invasion,tumor cell proliferation,and overactive immune responses.Due to intricate and diverse chemical structures,natural products and their derivatives have become an invaluable source inspiring innovative drug discovery.In addition,some natural products have been applied in clinical practice for infection,cancer,and autoimmunity over thousands of years and their promising curative effects and safety have been well-accepted.However,whether these natural products are primarily targeting type I IFN signaling and specific molecular targets involved are not fully elucidated.In the current review,we thoroughly summarize recent advances in the pharmacology researches of natural products for their type I IFN activity,including both agonism/activation and antagonism/inhibition,and their potential application as therapies.Furthermore,the source and chemical nature of natural products with type I IFN activity are highlighted and their specific molecular targets in the type I IFN pathway and mode of action are classified.In conclusion,natural products possessing type I IFN activity represent promising therapeutic strategies and have a bright prospect in the treatment of infection,cancer,and autoimmune diseases.展开更多
文摘为了对发酵桑叶茶工艺条件进行优化并探究桑叶茶经发酵后抗氧化活性的变化,该试验通过响应面分析法确定发酵桑叶茶在发酵温度、发酵时间和黑曲霉接种量3种变量条件下的最佳工艺条件,并测定发酵桑叶茶生物活性物质含量及其抗氧化性。结果表明,最佳工艺条件为发酵时间5 d 16 h,发酵温度30℃,接种量16.55%,此条件下所制发酵桑叶茶感官评分高达90分,且其总黄酮、总多糖、多酚、游离氨基酸、芦丁、槲皮素含量比桑叶绿茶分别升高了14.39%、12.67%、2.90%、3.46%、81.13%、19.35%,还检测到了含量7.55%±0.37%的茶褐素。此外,1/1000(g/mL)浓度下发酵桑叶茶对羟基自由基、DPPH自由基、超氧自由基的清除率和对二价铁离子的螯合率分别是桑叶绿茶的1.16倍、1.07倍、1.06倍和0.82倍,该研究结果为今后桑叶的进一步开发与应用提供理论基础和参考。
基金This work was supported by Beijing Nova Program of Science&Technology(Nos.Z201100006820025 and Z211100002121167)the Young Elite Scientists Sponsorship Program from CACM(No.CACM-2020-QNRC2-04)the National Natural Science Foundation of China(No.82004029).
文摘Type I interferon(IFN)is considered as a bridge between innate and adaptive immunity.Proper activation or inhibition of type I IFN signaling is essential for host defense against pathogen invasion,tumor cell proliferation,and overactive immune responses.Due to intricate and diverse chemical structures,natural products and their derivatives have become an invaluable source inspiring innovative drug discovery.In addition,some natural products have been applied in clinical practice for infection,cancer,and autoimmunity over thousands of years and their promising curative effects and safety have been well-accepted.However,whether these natural products are primarily targeting type I IFN signaling and specific molecular targets involved are not fully elucidated.In the current review,we thoroughly summarize recent advances in the pharmacology researches of natural products for their type I IFN activity,including both agonism/activation and antagonism/inhibition,and their potential application as therapies.Furthermore,the source and chemical nature of natural products with type I IFN activity are highlighted and their specific molecular targets in the type I IFN pathway and mode of action are classified.In conclusion,natural products possessing type I IFN activity represent promising therapeutic strategies and have a bright prospect in the treatment of infection,cancer,and autoimmune diseases.
