以不同年份铁皮石斛(Dendrobium officinale Kimura et Migo)的茎为材料,对其进行多糖、单糖以及木质素含量的测定以及多元线性回归分析,同时对铁皮石斛的茎进行番红-固绿和Mäule染色,并利用FTIR、1H-NMR技术对铁皮石斛茎中木质素...以不同年份铁皮石斛(Dendrobium officinale Kimura et Migo)的茎为材料,对其进行多糖、单糖以及木质素含量的测定以及多元线性回归分析,同时对铁皮石斛的茎进行番红-固绿和Mäule染色,并利用FTIR、1H-NMR技术对铁皮石斛茎中木质素单体的类型进行鉴定。结果显示,铁皮石斛茎木质素的积累与葡萄糖含量呈显著正相关,与多糖含量、甘露糖含量及甘露糖和葡萄糖的比值呈显著负相关,说明木质素的沉积对多糖的积累有一定影响。铁皮石斛茎维管束的木质部和鞘均含有木质素。随着年份的增加,铁皮石斛茎的维管束鞘厚壁细胞厚度增加,维管束鞘的发达程度增大。Mäule染色及FTIR和1H-NMR分析结果显示,铁皮石斛的茎主要含有愈创木基型(G型)和紫丁香基型(S型)木质素,同时含有微量的对羟基苯基型(H型)木质素。G型木质素主要分布在维管束的木质部,S型木质素主要分布在维管束鞘厚壁细胞中。铁皮石斛茎S/G比值随年份增加呈上升趋势。展开更多
Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the Gossypium sp. considerably influence the infil...Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the Gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A （shallow-lobed leaf） and sea-island cotton genotype ISR （deep-lobed leaf） to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper （HD-ZIPI） from a class I homologous domain factor Gorai.OO2G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL 1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides （BR） treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.展开更多
文摘以不同年份铁皮石斛(Dendrobium officinale Kimura et Migo)的茎为材料,对其进行多糖、单糖以及木质素含量的测定以及多元线性回归分析,同时对铁皮石斛的茎进行番红-固绿和Mäule染色,并利用FTIR、1H-NMR技术对铁皮石斛茎中木质素单体的类型进行鉴定。结果显示,铁皮石斛茎木质素的积累与葡萄糖含量呈显著正相关,与多糖含量、甘露糖含量及甘露糖和葡萄糖的比值呈显著负相关,说明木质素的沉积对多糖的积累有一定影响。铁皮石斛茎维管束的木质部和鞘均含有木质素。随着年份的增加,铁皮石斛茎的维管束鞘厚壁细胞厚度增加,维管束鞘的发达程度增大。Mäule染色及FTIR和1H-NMR分析结果显示,铁皮石斛的茎主要含有愈创木基型(G型)和紫丁香基型(S型)木质素,同时含有微量的对羟基苯基型(H型)木质素。G型木质素主要分布在维管束的木质部,S型木质素主要分布在维管束鞘厚壁细胞中。铁皮石斛茎S/G比值随年份增加呈上升趋势。
基金supported by the Genetically Modified Organisms Breeding Major Projects,China (2016ZX0800 5004, 2016ZX08009003-003-004)the National Natural Science Foundation of China (31601349)the Innovation Program of Chinese Academy of Agricultural Sciences
文摘Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the Gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A （shallow-lobed leaf） and sea-island cotton genotype ISR （deep-lobed leaf） to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper （HD-ZIPI） from a class I homologous domain factor Gorai.OO2G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL 1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides （BR） treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.
