使用相似原理构建缩尺模型是探究大型结构性能的最基本和最优途径。基于PATRAN软件的PCL语言与ISIGHT软件,提出一种联合静动力学、拓扑优化与多目标优化的集装箱相似畸变模型构建方法。利用拓扑优化的SIMP法(Solid Isotropic Material w...使用相似原理构建缩尺模型是探究大型结构性能的最基本和最优途径。基于PATRAN软件的PCL语言与ISIGHT软件,提出一种联合静动力学、拓扑优化与多目标优化的集装箱相似畸变模型构建方法。利用拓扑优化的SIMP法(Solid Isotropic Material with Penalization)得到缩尺集装箱墙壁结构的最优材料分布,既保证了结构性能又降低了缩尺模型的重量;基于全局多目标算法建立优化模型,采用有限元方法对集装箱性能进行分析,以结构刚度和一阶模态频率为目标函数进行寻优,得到相似模型的最优设计参数。最终利用有限元分析验证该模型的合理性。结果表明:该方法具有一定的通用性,能为其它类似结构的相似模型构建提供参考。展开更多
The technology of homology cloning and anchored PCR was used to clone the IL-1β gene from the Japanese sea perch （Lateolabrax iaponicus）. The full-length cDNA of sea perch IL-1β was 1 310 bp, including a 5＇ untra...The technology of homology cloning and anchored PCR was used to clone the IL-1β gene from the Japanese sea perch （Lateolabrax iaponicus）. The full-length cDNA of sea perch IL-1β was 1 310 bp, including a 5＇ untranslated regiop （UTR） of 136 bp, a 3＇ UTR ot 430 bp, and an ORF of 774 bp encoding a polypeptide of 258 amino acids with an estimated molecular mass of 29.31 kDa. The searches for nucleotides and protein sequence similarities with the BLAST analysis indicated that the deduced amino acid sequence of sea perch IL-1β was homological to the IL-1β in other fish species and even the mammalian. Conserved signature sequences of the IL-1β gene family were found in the sea perch IL-1β deduced amino acid sequence. Temporal expressions of the IL-1β gene in LPS or iridovirus challenged group and in control group were measured by the semi-quantitative RT-PCR. The mRNA transcripts of IL-1β could be detected in head-kidney, spleen, liver, gill and heart of the healthy individuals, and the expression level of IL-1β in head-kidney, spleen and gill was higher than that in liver and heart, but it was hard to be detected in the brain. After being stimulated by the LPS or iridovirus, the IL-1β expression in most of examined tissues was up-regulated, and also could be detected in the brain. These results indicated that the expression of sea perch IL-1β was constitutive and could be up-regulated by immune effector stimulation. Therefore the sea perch IL-1β could play a critical role in the host-pathogen interaction.展开更多
The relationship between the emission of pollutant and economic growth has attracted a lot of attention in the environmental debate of the recent decades. Based on some theoretical and empirical research on environmen...The relationship between the emission of pollutant and economic growth has attracted a lot of attention in the environmental debate of the recent decades. Based on some theoretical and empirical research on environmental Kuznets curve （EKC）, this paper introduces the environmental technical innovation and environmental investment into Salow growth model to discuss the relationship between GDP per capital and the emission of pollutant By the dynamic simulation and parameters analysis, the results of the model indicate： （1） when ＂green＂ technical progress and environmental investment are fixed, the relationship between GDP per capital and the emission shows the linear relationship; （2） ＂green＂ technical progress can lead to the positive growth rates with a decreasing level of emission, which is compatible with an EKC; （3） the proportion of the environmental investment can lead the different growth rates and level of emission. These results can explain that developing countries are ＂too poor to be green＂.展开更多
文摘使用相似原理构建缩尺模型是探究大型结构性能的最基本和最优途径。基于PATRAN软件的PCL语言与ISIGHT软件,提出一种联合静动力学、拓扑优化与多目标优化的集装箱相似畸变模型构建方法。利用拓扑优化的SIMP法(Solid Isotropic Material with Penalization)得到缩尺集装箱墙壁结构的最优材料分布,既保证了结构性能又降低了缩尺模型的重量;基于全局多目标算法建立优化模型,采用有限元方法对集装箱性能进行分析,以结构刚度和一阶模态频率为目标函数进行寻优,得到相似模型的最优设计参数。最终利用有限元分析验证该模型的合理性。结果表明:该方法具有一定的通用性,能为其它类似结构的相似模型构建提供参考。
文摘The technology of homology cloning and anchored PCR was used to clone the IL-1β gene from the Japanese sea perch （Lateolabrax iaponicus）. The full-length cDNA of sea perch IL-1β was 1 310 bp, including a 5＇ untranslated regiop （UTR） of 136 bp, a 3＇ UTR ot 430 bp, and an ORF of 774 bp encoding a polypeptide of 258 amino acids with an estimated molecular mass of 29.31 kDa. The searches for nucleotides and protein sequence similarities with the BLAST analysis indicated that the deduced amino acid sequence of sea perch IL-1β was homological to the IL-1β in other fish species and even the mammalian. Conserved signature sequences of the IL-1β gene family were found in the sea perch IL-1β deduced amino acid sequence. Temporal expressions of the IL-1β gene in LPS or iridovirus challenged group and in control group were measured by the semi-quantitative RT-PCR. The mRNA transcripts of IL-1β could be detected in head-kidney, spleen, liver, gill and heart of the healthy individuals, and the expression level of IL-1β in head-kidney, spleen and gill was higher than that in liver and heart, but it was hard to be detected in the brain. After being stimulated by the LPS or iridovirus, the IL-1β expression in most of examined tissues was up-regulated, and also could be detected in the brain. These results indicated that the expression of sea perch IL-1β was constitutive and could be up-regulated by immune effector stimulation. Therefore the sea perch IL-1β could play a critical role in the host-pathogen interaction.
文摘The relationship between the emission of pollutant and economic growth has attracted a lot of attention in the environmental debate of the recent decades. Based on some theoretical and empirical research on environmental Kuznets curve （EKC）, this paper introduces the environmental technical innovation and environmental investment into Salow growth model to discuss the relationship between GDP per capital and the emission of pollutant By the dynamic simulation and parameters analysis, the results of the model indicate： （1） when ＂green＂ technical progress and environmental investment are fixed, the relationship between GDP per capital and the emission shows the linear relationship; （2） ＂green＂ technical progress can lead to the positive growth rates with a decreasing level of emission, which is compatible with an EKC; （3） the proportion of the environmental investment can lead the different growth rates and level of emission. These results can explain that developing countries are ＂too poor to be green＂.
