Nine strains of ammonia nitrogen degradation strains from C1 to C9 were isolated from industrial wastewater to study their degradation and conversion of ammonia nitrogen. The results showed that C2 strain with a high ...Nine strains of ammonia nitrogen degradation strains from C1 to C9 were isolated from industrial wastewater to study their degradation and conversion of ammonia nitrogen. The results showed that C2 strain with a high degradation activiity of ammonia nitrogen, and the ammonia nitrogen degradation rate of the activated C2 strain was 93% within 24 h when the initial concentration of ammonia nitrogen was 200 mg/L under the conditions of inoculation 10%, temperature 35?C, pH 7.0, rotation 200 r/min. And C2 was identified as Bacillus amyloliquefaciens.展开更多
The cytochrome P450 （Cyt-P450） proteins from the fat body and midgut of the cotton bollworm, Helicoverpa armigera, were respectively partially purified by a set of purification procedures including differential cent...The cytochrome P450 （Cyt-P450） proteins from the fat body and midgut of the cotton bollworm, Helicoverpa armigera, were respectively partially purified by a set of purification procedures including differential centrifugation, solubilization of CHAPS, protein precipitation by PEG precipitation and DE-32 column chromatography. The CytP450 was detected by methods of CO difference spectrum and SDS-PAGE. Fraction of detergent solubilized microsomes from the fat body of H. armigera was purified more than 17-fold. Three protein bands were detected by SDS-PAGE with molecular masses of 70 600, 63 300 and 571 200Da. It is possible that the proteins with molecular mass of 63 300 and 571 200Da were the isozymes of Cyt-P450.展开更多
文摘Nine strains of ammonia nitrogen degradation strains from C1 to C9 were isolated from industrial wastewater to study their degradation and conversion of ammonia nitrogen. The results showed that C2 strain with a high degradation activiity of ammonia nitrogen, and the ammonia nitrogen degradation rate of the activated C2 strain was 93% within 24 h when the initial concentration of ammonia nitrogen was 200 mg/L under the conditions of inoculation 10%, temperature 35?C, pH 7.0, rotation 200 r/min. And C2 was identified as Bacillus amyloliquefaciens.
文摘The cytochrome P450 （Cyt-P450） proteins from the fat body and midgut of the cotton bollworm, Helicoverpa armigera, were respectively partially purified by a set of purification procedures including differential centrifugation, solubilization of CHAPS, protein precipitation by PEG precipitation and DE-32 column chromatography. The CytP450 was detected by methods of CO difference spectrum and SDS-PAGE. Fraction of detergent solubilized microsomes from the fat body of H. armigera was purified more than 17-fold. Three protein bands were detected by SDS-PAGE with molecular masses of 70 600, 63 300 and 571 200Da. It is possible that the proteins with molecular mass of 63 300 and 571 200Da were the isozymes of Cyt-P450.
