To obtain the maximum angiotensin-I converting enzyme(ACE) inhibitory activity, the protein hydrolysis conditions of the jellyfish Rhopilema hispidum were optimized using response surface methodology(RSM). Trypsin was...To obtain the maximum angiotensin-I converting enzyme(ACE) inhibitory activity, the protein hydrolysis conditions of the jellyfish Rhopilema hispidum were optimized using response surface methodology(RSM). Trypsin was selected to produce R. hispidum protein hydrolysates(RPH) with ACE inhibitory activity. The optimal parameters for producing protein hydrolysates with the highest ACE inhibitory activity were as follows: hydrolysis time 5 h, hydrolysis temperature 50℃, and the enzyme-to-substrate ratio 6%. Under these conditions, the ACE inhibitory rate of RPH could reach 64.28% ± 5.72%. In addition, RPH contained high levels of Gly, Glu, Pro, Ala, Asp and Arg, with a molecular weight distribution range of 0.32–6.84 kDa. The following three novel ACE inhibitory peptides were isolated and identified: Ile-Gly-Glu-Thr-Gly-Pro, Gly-Ala-Thr-Gly-Pro-Ala-Gly-Tyr-Val and Gly-AlaPhe-Gly-Pro-Gly-Gly-Leu-Val-Gly-Arg-Pro. The IC_(50) values of the ACE inhibitory activity of these three purified peptides were 19.07, 27.42 and 31.26 μmol L^(-1), respectively. These results suggested that proteins and peptides isolated from R. hispidum could be utilized as antihypertensive functional food sources.展开更多
Two new flavonoid glycosides, named viscumneoside XⅡ(1), and viscumneoside XⅢ(2);a new dihydrogen flavonoid glycoside product named viscumneoside XⅣ(3), were isolated from the aerial part of Viscum album, along wit...Two new flavonoid glycosides, named viscumneoside XⅡ(1), and viscumneoside XⅢ(2);a new dihydrogen flavonoid glycoside product named viscumneoside XⅣ(3), were isolated from the aerial part of Viscum album, along with seven known compounds(4-10). Their structures were identified by analysis of spectroscopic data. In addition, cytotoxicity assay showed that 1, 2 and 3 possessed significant inhibitory activities against C6, A549 and MDA-MB-231(the inhibition rate arrived about 50%, 70% and74% respectively with IC50 ≤ 60.00 μmol·L^-1), while the inhibition of TF-1 and Hela was not significant.展开更多
In the present study,two new acetylene conjugate compounds,dibutyl(2Z,6Z)-octa-2,6-dien-4-yne dioate(1),and dibutyl(2E,6E)-octa-2,6-dien-4-yne dioate(2),were isolated from the dry stem leaves of Viscum album,along wit...In the present study,two new acetylene conjugate compounds,dibutyl(2Z,6Z)-octa-2,6-dien-4-yne dioate(1),and dibutyl(2E,6E)-octa-2,6-dien-4-yne dioate(2),were isolated from the dry stem leaves of Viscum album,along with nine known compounds(3–11).Their structures were confirmed on the basis of spectroscopic data.Compounds 1 and 8 showed antioxidant activity against xanthine oxidase(XOD)and 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydroxyl(DPPH),with the IC_(50) of 1.22 and 1.33μmol·L^(–1),and the SC_(50 )of 4.34 and 8.22μmol·L^(–1),respectively.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 81230057, 81372615, 81472262, 41306133 and 81200264)the Emerging Cutting-Edge Technology Joint Research projects of Shanghai (No. SHDC12012106)+6 种基金the Tongji University Subject Pilot Program (No. 162385)partly funded by the Shanghai Municipal Health and Family Planning Commission Project (Nos. 201540027 and 20174Y0236)the seed fund program of Shanghai University of Medicine & Health Sciences (No. HSMF-17-22-031)Excellent Young Medical Expert of Shanghai (No. 2017YQ048)Shangha Natural Science Foundation (No. 18ZR1431700)China Postdoctoral Science Foundation (No. 2017M610278)the Key Research and Developing Plan of Shandong Province (No. 2015GSF115015)
文摘To obtain the maximum angiotensin-I converting enzyme(ACE) inhibitory activity, the protein hydrolysis conditions of the jellyfish Rhopilema hispidum were optimized using response surface methodology(RSM). Trypsin was selected to produce R. hispidum protein hydrolysates(RPH) with ACE inhibitory activity. The optimal parameters for producing protein hydrolysates with the highest ACE inhibitory activity were as follows: hydrolysis time 5 h, hydrolysis temperature 50℃, and the enzyme-to-substrate ratio 6%. Under these conditions, the ACE inhibitory rate of RPH could reach 64.28% ± 5.72%. In addition, RPH contained high levels of Gly, Glu, Pro, Ala, Asp and Arg, with a molecular weight distribution range of 0.32–6.84 kDa. The following three novel ACE inhibitory peptides were isolated and identified: Ile-Gly-Glu-Thr-Gly-Pro, Gly-Ala-Thr-Gly-Pro-Ala-Gly-Tyr-Val and Gly-AlaPhe-Gly-Pro-Gly-Gly-Leu-Val-Gly-Arg-Pro. The IC_(50) values of the ACE inhibitory activity of these three purified peptides were 19.07, 27.42 and 31.26 μmol L^(-1), respectively. These results suggested that proteins and peptides isolated from R. hispidum could be utilized as antihypertensive functional food sources.
基金supported by the National Science and Technology Development Fund(No.2009ZX09301-007)the Scientific Research Projects of Shannxi Academy of Sciences(No.2016K-17)+4 种基金the Projects of Shaanxi Provincial Science and Technology Department(No.2016SF-264)Special Fund for Agro-scientific Research in The Public Interest(Grant 389,No.201203062)Support Plan for Young 392 Talents of Science and Technology Association of Colleges and Universities in Shaanxi Province 393(No.20150105)Key Research and Development Plan Project of Shaanxi Province(No.2018SF-034)Scientific Research from Shaanxi Provincial Department of Education(Nos.14JS100 and 17JK0243)
文摘Two new flavonoid glycosides, named viscumneoside XⅡ(1), and viscumneoside XⅢ(2);a new dihydrogen flavonoid glycoside product named viscumneoside XⅣ(3), were isolated from the aerial part of Viscum album, along with seven known compounds(4-10). Their structures were identified by analysis of spectroscopic data. In addition, cytotoxicity assay showed that 1, 2 and 3 possessed significant inhibitory activities against C6, A549 and MDA-MB-231(the inhibition rate arrived about 50%, 70% and74% respectively with IC50 ≤ 60.00 μmol·L^-1), while the inhibition of TF-1 and Hela was not significant.
基金supported by the National Science and Technology Development Fund(No.2009ZX09301-007)
文摘In the present study,two new acetylene conjugate compounds,dibutyl(2Z,6Z)-octa-2,6-dien-4-yne dioate(1),and dibutyl(2E,6E)-octa-2,6-dien-4-yne dioate(2),were isolated from the dry stem leaves of Viscum album,along with nine known compounds(3–11).Their structures were confirmed on the basis of spectroscopic data.Compounds 1 and 8 showed antioxidant activity against xanthine oxidase(XOD)and 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydroxyl(DPPH),with the IC_(50) of 1.22 and 1.33μmol·L^(–1),and the SC_(50 )of 4.34 and 8.22μmol·L^(–1),respectively.
