The present study aimed at isolation and purification of the bioactive terpenoids from the herb of Leonurus japonicus by chromatographic separations such as silica gel, sephadex LH-20 and C18 reversed phase silica gel...The present study aimed at isolation and purification of the bioactive terpenoids from the herb of Leonurus japonicus by chromatographic separations such as silica gel, sephadex LH-20 and C18 reversed phase silica gel, as well as preparative HPLC. As a result, leojaponic acids A(1, C_(17)H_(24)O_4) and B(2, C_(18)H_(26)O_4), two homologous terpenoids, together with(-)-loliolide(3), 1-(3-ethylphenyl) ethane-1, 2-diol(4) and dibutyl phthalate(5), were isolated from the Et OH extract of L. japonicus. All the chemical structures of the isolates were elucidated on the basis of 1D and 2D NMR analyses. Compounds 1 and 2 were new terpenoids, and Compounds 3 and 4 were isolated and identified for the first time from this plant. In addition, the a-glucosidase and tyrosinase inhibitory activity of the new compounds were evaluated.展开更多
基金supported by the National Natural Science Foundation of China(U1304825,201302004)the National Key Technology R&D Program(2012BAD36B01-02)
文摘The present study aimed at isolation and purification of the bioactive terpenoids from the herb of Leonurus japonicus by chromatographic separations such as silica gel, sephadex LH-20 and C18 reversed phase silica gel, as well as preparative HPLC. As a result, leojaponic acids A(1, C_(17)H_(24)O_4) and B(2, C_(18)H_(26)O_4), two homologous terpenoids, together with(-)-loliolide(3), 1-(3-ethylphenyl) ethane-1, 2-diol(4) and dibutyl phthalate(5), were isolated from the Et OH extract of L. japonicus. All the chemical structures of the isolates were elucidated on the basis of 1D and 2D NMR analyses. Compounds 1 and 2 were new terpenoids, and Compounds 3 and 4 were isolated and identified for the first time from this plant. In addition, the a-glucosidase and tyrosinase inhibitory activity of the new compounds were evaluated.
