This study was to investigate the effects of three exogenous substances on chemical constituents of Isatis indigotica leavesand their efficacy in alleviating drought stress, and explore the methods of applying exogeno...This study was to investigate the effects of three exogenous substances on chemical constituents of Isatis indigotica leavesand their efficacy in alleviating drought stress, and explore the methods of applying exogenous substances to efficient cultivationof Isatis indigotica. Polyethylene glycol (PEG) was used to simulate drought stress to deal with seeds of Isatis indigotica at thegermination stage (concentration: 0, 10%, 15%, and 20%). Simultaneous operation of exogenous growth regulators [microbialinoculum (MI), γ-aminobutyric acid (GABA) and salicylic acid (SA)] and PEG were implemented in seeds of Isatis indigotica.The effects of drought stress and the mitigation of exogenous substances were observed by statistics of seed germination potential,germination rate, hypocotyl length, and radicle length of each treatment. The effects of exogenous substances on the content ofalkaloids, crude protein and free amino acids in the leaves of Isatis indigotica grown in a greenhouse were determined after sprayingexogenous substances on the plants. The differences of germination potential, germination rate, hypocotyl length, and radicle lengthamong 15% PEG stress treatment, 10% PEG stress treatment and the control were significant (P<0.05). According to the predesignedgermination standard, the seeds did not germinate under 20% PEG stress treatment. When the PEG concentration was 15%, the resultsof seed germination potential and germination rate after adding MI were significantly different from those under stress alone (P<0.05).When exposed to 10% PEG stress, the supplementation of GABA led to a notable increase in radicle length of Isatis indigotica seeds,showing significant differences compared to other three treatments. The application of MI and GABA under 15% PEG stress resultedin a significant increase in the radicle and hypocotyl length of Isatis indigotica seeds compared to other two treatments. The contentof the total alkaloids in leaves of Isatis indigotica was significantly increased after spraying GABA. Meanwhile, the contents of crudeprotein and the total free amino acids were kept constant after spraying exogenous substances. Application of MI and GABA couldalleviate drought stress of Isatis indigotica. The content of the total alkaloids in leaves of Isatis indigotica could significantly increaseafter spraying GABA.展开更多
目的研究儿科重症监护病房(pediatric intensive care unit,PICU)住院医师职业倦怠的现状,探索降低职业倦怠的方法。方法2019年1月—2021年12月,采用问卷调查法、统计学分析方法对上海某三甲医院PICU住院医师的职业倦怠进行分析。结果...目的研究儿科重症监护病房(pediatric intensive care unit,PICU)住院医师职业倦怠的现状,探索降低职业倦怠的方法。方法2019年1月—2021年12月,采用问卷调查法、统计学分析方法对上海某三甲医院PICU住院医师的职业倦怠进行分析。结果回收有效问卷71份。研究对象职业倦怠量表中“情绪衰竭”“玩世不恭”“个人成就感低”三个维度得分分别为(3.55±0.94)分、(2.45±1.10)分、(2.90±0.65)分,有77.5%的医师存在中度职业倦怠,21.1%的医师存在重度职业倦怠。职业倦怠的两个分量表(情绪衰竭、玩世不恭)与内在工作满意度、外在工作满意度、总体工作满意度差异有统计学意义(P<0.05)。学历、年龄、工作时间、收入与PICU住院医生职业倦怠得分的差异有统计学意义(P<0.05)。其中研究生学历者、每周工作时间≥50小时者“情绪衰竭”得分更高,年龄≥30岁者、年收入<10万元者“个人成就感低”得分更高。结论PICU住院医师职业倦怠水平高,职业倦怠与工作满意度呈负相关,学历、年龄、工作时间、收入可影响职业倦怠水平,应采取措施降低职业倦怠,稳定医疗队伍。展开更多
基金the Doctoral Research Initiation Foundation of Changzhi Medical College(BS202005)。
文摘This study was to investigate the effects of three exogenous substances on chemical constituents of Isatis indigotica leavesand their efficacy in alleviating drought stress, and explore the methods of applying exogenous substances to efficient cultivationof Isatis indigotica. Polyethylene glycol (PEG) was used to simulate drought stress to deal with seeds of Isatis indigotica at thegermination stage (concentration: 0, 10%, 15%, and 20%). Simultaneous operation of exogenous growth regulators [microbialinoculum (MI), γ-aminobutyric acid (GABA) and salicylic acid (SA)] and PEG were implemented in seeds of Isatis indigotica.The effects of drought stress and the mitigation of exogenous substances were observed by statistics of seed germination potential,germination rate, hypocotyl length, and radicle length of each treatment. The effects of exogenous substances on the content ofalkaloids, crude protein and free amino acids in the leaves of Isatis indigotica grown in a greenhouse were determined after sprayingexogenous substances on the plants. The differences of germination potential, germination rate, hypocotyl length, and radicle lengthamong 15% PEG stress treatment, 10% PEG stress treatment and the control were significant (P<0.05). According to the predesignedgermination standard, the seeds did not germinate under 20% PEG stress treatment. When the PEG concentration was 15%, the resultsof seed germination potential and germination rate after adding MI were significantly different from those under stress alone (P<0.05).When exposed to 10% PEG stress, the supplementation of GABA led to a notable increase in radicle length of Isatis indigotica seeds,showing significant differences compared to other three treatments. The application of MI and GABA under 15% PEG stress resultedin a significant increase in the radicle and hypocotyl length of Isatis indigotica seeds compared to other two treatments. The contentof the total alkaloids in leaves of Isatis indigotica was significantly increased after spraying GABA. Meanwhile, the contents of crudeprotein and the total free amino acids were kept constant after spraying exogenous substances. Application of MI and GABA couldalleviate drought stress of Isatis indigotica. The content of the total alkaloids in leaves of Isatis indigotica could significantly increaseafter spraying GABA.
文摘目的研究儿科重症监护病房(pediatric intensive care unit,PICU)住院医师职业倦怠的现状,探索降低职业倦怠的方法。方法2019年1月—2021年12月,采用问卷调查法、统计学分析方法对上海某三甲医院PICU住院医师的职业倦怠进行分析。结果回收有效问卷71份。研究对象职业倦怠量表中“情绪衰竭”“玩世不恭”“个人成就感低”三个维度得分分别为(3.55±0.94)分、(2.45±1.10)分、(2.90±0.65)分,有77.5%的医师存在中度职业倦怠,21.1%的医师存在重度职业倦怠。职业倦怠的两个分量表(情绪衰竭、玩世不恭)与内在工作满意度、外在工作满意度、总体工作满意度差异有统计学意义(P<0.05)。学历、年龄、工作时间、收入与PICU住院医生职业倦怠得分的差异有统计学意义(P<0.05)。其中研究生学历者、每周工作时间≥50小时者“情绪衰竭”得分更高,年龄≥30岁者、年收入<10万元者“个人成就感低”得分更高。结论PICU住院医师职业倦怠水平高,职业倦怠与工作满意度呈负相关,学历、年龄、工作时间、收入可影响职业倦怠水平,应采取措施降低职业倦怠,稳定医疗队伍。
