The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concent...The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concentration of H2O2 in the range of 2.0×10^-5- 2.0×10^-7 mol/L, with a correlation coefficient of 0.999 1. The proposed meth od is simple and highly sensitive to a detection limit of 2.7 × 10^-8 mol/L. The relative standard deviations were within 5% for the determination of different concentrations of H2O2. Satisfied results were obtained in the determination of H2O2 in rainwater by this method, and the recoveries were at the range of 97.2%-105.4%.展开更多
A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium...A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.展开更多
基金Supported by the National Natural Science Foun-dation of China (20275027) the Natural Science Foundation ofHubei Province (2004ABA182) .
文摘The characteristic of Myoglobin as mimetic enzyme was studied and applied in the determination of HeO2 with o-phenylenediamine as substrate. The absorbanee obtained at 40 ℃ was found to be proportional to the concentration of H2O2 in the range of 2.0×10^-5- 2.0×10^-7 mol/L, with a correlation coefficient of 0.999 1. The proposed meth od is simple and highly sensitive to a detection limit of 2.7 × 10^-8 mol/L. The relative standard deviations were within 5% for the determination of different concentrations of H2O2. Satisfied results were obtained in the determination of H2O2 in rainwater by this method, and the recoveries were at the range of 97.2%-105.4%.
基金Supported by the National Natural Science Foundation of China(20675059)Research Foundation of Leshan Teachers’ College, China (Z0625)
文摘A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.
