【目的】通过测定113份板栗品种(系)的数量、质量和假质量性状,分析其遗传变异,比较不同组群之间的性状差异,将SSR标记与性状进行关联,获得更多与SSR标记显著关联的性状,挖掘优异的等位变异位点,为开展板栗分子辅助育种的研究提供参考...【目的】通过测定113份板栗品种(系)的数量、质量和假质量性状,分析其遗传变异,比较不同组群之间的性状差异,将SSR标记与性状进行关联,获得更多与SSR标记显著关联的性状,挖掘优异的等位变异位点,为开展板栗分子辅助育种的研究提供参考。【方法】测定并分析板栗总苞和坚果的38个数量、质量和假质量性状,利用SPSS和Graphpad软件对数量性状进行差异显著性和相关性分析,基于SSR标记进行遗传多样性分析,最后用TASSEL 2.1软件通过一般线性模型(general linear model,GLM)和混合线性模型(mixed linear model,MLM)分别对性状和标记进行关联分析。【结果】在遗传多样性分析中,21对SSR引物的有效等位基因数(N;)、Shannon指数(I)、多态性信息含量(PIC>0.5)、观察杂合度(H;)和期望杂合度(H;)的平均值分别为3.164、1.269、0.589、0.593和0.635。根据群体结构分析分为2个主要组群,为了更好地比较性状差异,在聚类分析时将中间类型的品种单独划分为一组。在质量和假质量性状分析中,多样性指数变化范围为0.139—1.567,遗传多样性最高的性状是坚果光泽,最低的是底座接线;坚果形状、光泽、颜色等性状组群间频率分布差异明显。在数量性状分析中,变异系数的范围在3.96%—36.31%,苞重、总苞重和单粒重的变异系数均在30%以上,遗传变异程度高;果形指数和含水量变异系数均在10%以下,具有稳定的遗传特性;总苞和坚果的外观性状之间有强相关性,相关系数均在0.6以上;组1-组2和组2-组3在果实形状和重量中存在显著差异(P<0.05)。在关联分析中,GLM模型中有13个标记位点与18个表型性状极显著关联,表型变异解释率范围为15.12%—54.99%;MLM模型中有6个标记位点与7个表型性状极显著关联,表型变异解释率范围在8.66%—26.93%。【结论】本研究将SSR标记与表型性状进行关联分析,共发现13个标记位点与坚果单粒重等20个表型性状极显著关联,为开展板栗分子辅助育种的研究奠定了基础。展开更多
Endo-β-glucanases play vital roles in the regulation of pollen tube growth. Here, a previously identified endo-1,4-β-glucanase from Lilium longiflorum (lily), named LlpCel1, was expressed in Escherichia coli, purifi...Endo-β-glucanases play vital roles in the regulation of pollen tube growth. Here, a previously identified endo-1,4-β-glucanase from Lilium longiflorum (lily), named LlpCel1, was expressed in Escherichia coli, purified, and further investigated for its physiological function. The recombinant LlpCel1 protein hydrolyzed carboxy-methylcellulose (CMC) and exhibited activity towards laminarin from Eisenia. arborea and 1,3:1,4-β-glucan of barley. The pH for the optimum activity was 6.0 and the value of Km calculated from CMC was 5.0 mg/mL. Adding EDTA resulted in the total loss of the enzymatic activity, and this effect could be restored by the addition of Ca2+. Western blotting analysis showed that LlpCel1 protein was present in pollen grains and rehydrated pollen grains, and the amount of the protein was increased during pollen germinating, but not in the pollen tube. Consistently, the immunofluorescence labeling study with the antibody against LlpCel1 also indicated the presence of LlpCel1 at the begin-ning of germination, but not in the elongating pollen tube. Furthermore, incubation of LlpCel1 with pollen at the beginning of pollen germination increased the germination percentage and the length of pollen tube. All of these results suggested that LlpCel1 could play an important role in the regulation of lily pollen germination and the initiation of pollen tube growth.展开更多
文摘【目的】通过测定113份板栗品种(系)的数量、质量和假质量性状,分析其遗传变异,比较不同组群之间的性状差异,将SSR标记与性状进行关联,获得更多与SSR标记显著关联的性状,挖掘优异的等位变异位点,为开展板栗分子辅助育种的研究提供参考。【方法】测定并分析板栗总苞和坚果的38个数量、质量和假质量性状,利用SPSS和Graphpad软件对数量性状进行差异显著性和相关性分析,基于SSR标记进行遗传多样性分析,最后用TASSEL 2.1软件通过一般线性模型(general linear model,GLM)和混合线性模型(mixed linear model,MLM)分别对性状和标记进行关联分析。【结果】在遗传多样性分析中,21对SSR引物的有效等位基因数(N;)、Shannon指数(I)、多态性信息含量(PIC>0.5)、观察杂合度(H;)和期望杂合度(H;)的平均值分别为3.164、1.269、0.589、0.593和0.635。根据群体结构分析分为2个主要组群,为了更好地比较性状差异,在聚类分析时将中间类型的品种单独划分为一组。在质量和假质量性状分析中,多样性指数变化范围为0.139—1.567,遗传多样性最高的性状是坚果光泽,最低的是底座接线;坚果形状、光泽、颜色等性状组群间频率分布差异明显。在数量性状分析中,变异系数的范围在3.96%—36.31%,苞重、总苞重和单粒重的变异系数均在30%以上,遗传变异程度高;果形指数和含水量变异系数均在10%以下,具有稳定的遗传特性;总苞和坚果的外观性状之间有强相关性,相关系数均在0.6以上;组1-组2和组2-组3在果实形状和重量中存在显著差异(P<0.05)。在关联分析中,GLM模型中有13个标记位点与18个表型性状极显著关联,表型变异解释率范围为15.12%—54.99%;MLM模型中有6个标记位点与7个表型性状极显著关联,表型变异解释率范围在8.66%—26.93%。【结论】本研究将SSR标记与表型性状进行关联分析,共发现13个标记位点与坚果单粒重等20个表型性状极显著关联,为开展板栗分子辅助育种的研究奠定了基础。
基金Supported by the National Natural Science Foundation of China (Grant No. 30170090)
文摘Endo-β-glucanases play vital roles in the regulation of pollen tube growth. Here, a previously identified endo-1,4-β-glucanase from Lilium longiflorum (lily), named LlpCel1, was expressed in Escherichia coli, purified, and further investigated for its physiological function. The recombinant LlpCel1 protein hydrolyzed carboxy-methylcellulose (CMC) and exhibited activity towards laminarin from Eisenia. arborea and 1,3:1,4-β-glucan of barley. The pH for the optimum activity was 6.0 and the value of Km calculated from CMC was 5.0 mg/mL. Adding EDTA resulted in the total loss of the enzymatic activity, and this effect could be restored by the addition of Ca2+. Western blotting analysis showed that LlpCel1 protein was present in pollen grains and rehydrated pollen grains, and the amount of the protein was increased during pollen germinating, but not in the pollen tube. Consistently, the immunofluorescence labeling study with the antibody against LlpCel1 also indicated the presence of LlpCel1 at the begin-ning of germination, but not in the elongating pollen tube. Furthermore, incubation of LlpCel1 with pollen at the beginning of pollen germination increased the germination percentage and the length of pollen tube. All of these results suggested that LlpCel1 could play an important role in the regulation of lily pollen germination and the initiation of pollen tube growth.
