MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处...MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处理后均有不同程度的表达响应,且在抗病、感病品种间存在一定差异。通过茉莉酸、水杨酸和乙烯处理,发现OsMPKs基因至少受到1种激素的诱导表达,且对不同激素的处理响应存在差异。组织表达分析显示,OsMPK3、OsMPK6等6个OsMPKs基因在纹枯病菌主要侵染部位叶鞘和叶片中有较强表达。综合比较不同表达分析结果,发现一些可能参与调控水稻对纹枯病抗性的OsMPKs基因。展开更多
MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resis...MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice.To investigate whether miRNAs regulate the resistance to R.solani,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,respectively.By taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the libraries.Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R.solani challenge.Thirty-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction.Moreover,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to R.solani infection confirmed by Northern blot.Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R.solani.Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.展开更多
This article presents the successful experience in adopting the Isodewaxing technology in the lube oil unit of Shanghai Gaoqiao refinery. In line with the demand of different crude slate for different reaction depth, ...This article presents the successful experience in adopting the Isodewaxing technology in the lube oil unit of Shanghai Gaoqiao refinery. In line with the demand of different crude slate for different reaction depth, and good match between the optimal product quality and the yield, the refinery has make strenuous efforts to optimize the operation and reduce the energy consumption to manufacture high-quality hydrogenated base stocks.展开更多
文摘MAPK (mitogen-activated protein kinase)信号级联系统在调节植物生长发育和逆境胁迫中具有重要作用。以不同抗感纹枯病水平的水稻品种为材料,分析OsMPKs类基因对水稻纹枯病菌的侵染响应特征,发现供检测的17个OsMPKs基因对纹枯病菌处理后均有不同程度的表达响应,且在抗病、感病品种间存在一定差异。通过茉莉酸、水杨酸和乙烯处理,发现OsMPKs基因至少受到1种激素的诱导表达,且对不同激素的处理响应存在差异。组织表达分析显示,OsMPK3、OsMPK6等6个OsMPKs基因在纹枯病菌主要侵染部位叶鞘和叶片中有较强表达。综合比较不同表达分析结果,发现一些可能参与调控水稻对纹枯病抗性的OsMPKs基因。
基金the National Key Research and Development Program of China(Grant No.2016YFD0100601)National Natural Science Foundation of China(Grant No.31672013)+2 种基金FOK YING TUNG Education Foundation(Grant No.151026)High Talent Project of Yangzhou UniversityPriority Academic Program Development of Jiangsu Higher Education Institutions.
文摘MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses.However,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice.To investigate whether miRNAs regulate the resistance to R.solani,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,respectively.By taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the libraries.Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R.solani challenge.Thirty-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction.Moreover,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to R.solani infection confirmed by Northern blot.Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R.solani.Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.
文摘This article presents the successful experience in adopting the Isodewaxing technology in the lube oil unit of Shanghai Gaoqiao refinery. In line with the demand of different crude slate for different reaction depth, and good match between the optimal product quality and the yield, the refinery has make strenuous efforts to optimize the operation and reduce the energy consumption to manufacture high-quality hydrogenated base stocks.
