Objective: To investigate the anti-inflammatory activity of Radix Panacis quinquefolii extract(RPQE) and its therapeutic effects on inflammatory bowel disease(IBD). Methods: The 72-hour post-fertilization zebrafish wa...Objective: To investigate the anti-inflammatory activity of Radix Panacis quinquefolii extract(RPQE) and its therapeutic effects on inflammatory bowel disease(IBD). Methods: The 72-hour post-fertilization zebrafish was used to generate the local and systematic inflammation models through tail-amputation and lipopolysaccharide(LPS)-induction(100 μg/m L), respectively. The Tg(zlyz:EGFP) zebrafish was induced with 75 μg/m L 2,4,6-trinitrobenzene sulfonic acid(TNBS) for establishing the IBD model. The tail-amputated, LPS-, and TNBS-induced models were subjected to RPQE(ethanol fraction, 10–20 μg/m L) administration for 12 and 24 h, respectively. Anti-inflammatory activity of RPQE was evaluated by detecting migration and aggregation of leukocytes and expression of inflammation-related genes. Meanwhile, TNBS-induced fish were immersed in 0.2%(W/V) calcein for 1.5 h and RPQE for 12 h before photographing to analyze the intestinal efflux efficiency(IEE). Moreover, the expression of inflammation-related genes in these fish was detected by quantitative polymerase chain reaction. Results: Subject to RPQE administration, the migration and aggregation of leukocytes were significantly alleviated in 3 zebrafish models(P<0.01). Herein, RPQE ameliorated TNBS-induced IBD with respect to a significantly reduced number of leukocytes, improved IEE, and inhibited gene expression of pro-inflammatory factors(P<0.05 or P<0.01). Conclusion: RPQE exhibited therapeutic effects on IBD by inhibiting inflammation.展开更多
基金Supported by Shandong Provincial Natural Science Foundation(No.ZR2020YQ60,ZR2021QH274)Jinan Talent Project for University(No.2021GXRC047)+2 种基金the Major Scientific and Technological Innovation Project of Shandong Province(No.2019JZZY020905)Science,Education and Industry Integration Innovation Pilot Project of Qilu University of Technology(Shandong Academy of Sciences,No.2022PYI016)Key R&D Project of Shandong Province(No.2021CXGC010507)。
文摘Objective: To investigate the anti-inflammatory activity of Radix Panacis quinquefolii extract(RPQE) and its therapeutic effects on inflammatory bowel disease(IBD). Methods: The 72-hour post-fertilization zebrafish was used to generate the local and systematic inflammation models through tail-amputation and lipopolysaccharide(LPS)-induction(100 μg/m L), respectively. The Tg(zlyz:EGFP) zebrafish was induced with 75 μg/m L 2,4,6-trinitrobenzene sulfonic acid(TNBS) for establishing the IBD model. The tail-amputated, LPS-, and TNBS-induced models were subjected to RPQE(ethanol fraction, 10–20 μg/m L) administration for 12 and 24 h, respectively. Anti-inflammatory activity of RPQE was evaluated by detecting migration and aggregation of leukocytes and expression of inflammation-related genes. Meanwhile, TNBS-induced fish were immersed in 0.2%(W/V) calcein for 1.5 h and RPQE for 12 h before photographing to analyze the intestinal efflux efficiency(IEE). Moreover, the expression of inflammation-related genes in these fish was detected by quantitative polymerase chain reaction. Results: Subject to RPQE administration, the migration and aggregation of leukocytes were significantly alleviated in 3 zebrafish models(P<0.01). Herein, RPQE ameliorated TNBS-induced IBD with respect to a significantly reduced number of leukocytes, improved IEE, and inhibited gene expression of pro-inflammatory factors(P<0.05 or P<0.01). Conclusion: RPQE exhibited therapeutic effects on IBD by inhibiting inflammation.
