为研究京大戟醋制前后不同极性部位对斑马鱼胚胎肝、胃肠毒性的影响,实验以95%乙醇提取生、醋京大戟,经石油醚、二氯甲烷、乙酸乙酯依次萃取得不同极性部位。将受精后12 h (12 hpf)的斑马鱼胚胎暴露于不同浓度的溶液中持续至受精96 h (9...为研究京大戟醋制前后不同极性部位对斑马鱼胚胎肝、胃肠毒性的影响,实验以95%乙醇提取生、醋京大戟,经石油醚、二氯甲烷、乙酸乙酯依次萃取得不同极性部位。将受精后12 h (12 hpf)的斑马鱼胚胎暴露于不同浓度的溶液中持续至受精96 h (96 hpf),对斑马鱼胚胎的肝、胃肠进行毒性观察。结果显示,毒性随着样品浓度的增加而增强。京大戟醋制前后各极性部位对斑马鱼胚胎的肝、胃肠毒性主要表现为肝脏发育缓慢、肝面积变小、肝脏变性发黑、卵黄囊水肿、吸收延迟、出血、胃肠道蠕动减缓、胃肠道杯状细胞分泌功能异常等,醋制后毒性反应降低。其中石油醚和二氯甲烷部位肝、胃肠毒性作用较为明显,乙酸乙酯部位毒性较弱。以上结果说明,京大戟醋制后毒性降低,且石油醚、二氯甲烷部位是其产生肝、胃肠毒性的主要部位。展开更多
Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control gro...Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control group(0.5%carboxymethylcellulose sodium,20 mL/kg),positive control group(0.1%mixture of carbon tetrachloride in soybean oil,20 mL/kg),RK group(the ethyl acetate extracts of RK,250 g crude drug/kg)and VRK group(the ethyl acetate extracts of VRK,250 g crude drug/kg)with 10 mice per group.All mice were administered orally by gavage daily for 7 continuous days.The morphology of liver tissues was examined to assess the liver injury by a transmission electron microscope.Hepatocyte apoptosis in vivo was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling(TUNEL)assay.Immunohistochemical technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways,including B-cell lymphoma(Bcl-2)and caspase-3,as well as the expression of inflammatory mediators,including nuclear factor kappa B(NF-κB)and intercellular adhesion molecule-1(ICAM-1).Results Liver injury and hepatocyte apoptosis were observed in RK mice,and the liver injury were significantly reduced in VRK-treated mice.In immunohistochemistry study,compared with the negative control group,RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3,NF-κB and ICAM-1(all P<0.01).Compared with the RK group,VRK group induced significant increase on Bcl-2 protein expression,and decreased the caspase-3,NF-κB and ICAM-1 protein expression(P<0.05 or <0.01).Conclusion The mechanism of reduced hepatotoxicity of VRK may be associated with the reduced inflammation,regulation of antiapoptotic and proapoptotic mediators in the mitochondrial pathway.展开更多
文摘为研究京大戟醋制前后不同极性部位对斑马鱼胚胎肝、胃肠毒性的影响,实验以95%乙醇提取生、醋京大戟,经石油醚、二氯甲烷、乙酸乙酯依次萃取得不同极性部位。将受精后12 h (12 hpf)的斑马鱼胚胎暴露于不同浓度的溶液中持续至受精96 h (96 hpf),对斑马鱼胚胎的肝、胃肠进行毒性观察。结果显示,毒性随着样品浓度的增加而增强。京大戟醋制前后各极性部位对斑马鱼胚胎的肝、胃肠毒性主要表现为肝脏发育缓慢、肝面积变小、肝脏变性发黑、卵黄囊水肿、吸收延迟、出血、胃肠道蠕动减缓、胃肠道杯状细胞分泌功能异常等,醋制后毒性反应降低。其中石油醚和二氯甲烷部位肝、胃肠毒性作用较为明显,乙酸乙酯部位毒性较弱。以上结果说明,京大戟醋制后毒性降低,且石油醚、二氯甲烷部位是其产生肝、胃肠毒性的主要部位。
基金the National Natural Science Foundation of China(No.81673599,81503250,and 21705081)the Natural Science Research Program of Jiangsu Higher Education Institutions(No.15KJB360009)+2 种基金the Natural Science Foundation of Jiangsu Province(No.BK20161037)the Project Foundation of the Priority Academic Program Development of Jiangsu Higher Education Institutions(No.PAPD-2014)Six Talent Peaks Program of Jiangsu Province(No.2016-YY-026)。
文摘Objective To investigate the mechanism of Radix Kansui(RK)stir-fried with vinegar(VRK)decreased hepatotoxicity in mice.Methods According to a random number table,40 mice were randomly divided into negative control group(0.5%carboxymethylcellulose sodium,20 mL/kg),positive control group(0.1%mixture of carbon tetrachloride in soybean oil,20 mL/kg),RK group(the ethyl acetate extracts of RK,250 g crude drug/kg)and VRK group(the ethyl acetate extracts of VRK,250 g crude drug/kg)with 10 mice per group.All mice were administered orally by gavage daily for 7 continuous days.The morphology of liver tissues was examined to assess the liver injury by a transmission electron microscope.Hepatocyte apoptosis in vivo was determined by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nickend labeling(TUNEL)assay.Immunohistochemical technique was adopted to detect the expression of particular antiapoptotic and proapoptotic proteins in the mitochondrial pathways,including B-cell lymphoma(Bcl-2)and caspase-3,as well as the expression of inflammatory mediators,including nuclear factor kappa B(NF-κB)and intercellular adhesion molecule-1(ICAM-1).Results Liver injury and hepatocyte apoptosis were observed in RK mice,and the liver injury were significantly reduced in VRK-treated mice.In immunohistochemistry study,compared with the negative control group,RK inhibited dramatically the Bcl-2 protein expression and significantly increased the expression of caspase-3,NF-κB and ICAM-1(all P<0.01).Compared with the RK group,VRK group induced significant increase on Bcl-2 protein expression,and decreased the caspase-3,NF-κB and ICAM-1 protein expression(P<0.05 or <0.01).Conclusion The mechanism of reduced hepatotoxicity of VRK may be associated with the reduced inflammation,regulation of antiapoptotic and proapoptotic mediators in the mitochondrial pathway.
