目的基于生物信息学揭示益母草治疗卵巢癌的分子机制。方法本研究采用GEO数据库GPL96平台共3个基因芯片,和GeneCards数据库做交集靶点,作为卵巢癌的核心疾病靶点。中药靶点的获取采用中药系统药理学分析平台(Traditional Chinese medici...目的基于生物信息学揭示益母草治疗卵巢癌的分子机制。方法本研究采用GEO数据库GPL96平台共3个基因芯片,和GeneCards数据库做交集靶点,作为卵巢癌的核心疾病靶点。中药靶点的获取采用中药系统药理学分析平台(Traditional Chinese medicine system platform,TCMSP),依据口服利用度(Oral bioavailability,OB)≥30%,类药性(Drug-likeness,DL)≥0.18为益母草活性成分的筛选条件,将益母草中活性较高的成分筛选出来,并获取相应靶点蛋白。将卵巢癌的核心疾病靶点和益母草的靶点做线上Venn交集,将得到的共同靶点做生存分析,排除P>0.05的靶点,得到关键靶点;做关键靶点蛋白PPI互作网络图,DAVID数据库对关键靶点进行GO基因功能分析、KEGG通路分析。选取湖北中医药大学附属医院2018年1月—2020年1月手术治疗的60例卵巢组织标本,其中卵巢癌组织30例,正常卵巢组织30例,采用qRT-PCR法检测两组患者差异基因的表达变化。结果GeneCards数据库共记录卵巢癌6657个疾病靶点,与GPL96平台上基因芯片得到的疾病靶点进行比较,共得到487个卵巢癌疾病靶点。TCMSP数据库共记录益母草共204个靶点,与卵巢癌核心靶点做线上Venn分析,共得到22个共同靶点,将得到的共同靶点导入Kaplan Meier-Plotter进行在线生存分析,排除P>0.05的4个靶点,得到18个关键靶点;关键靶点PPI蛋白互作网络图显示,关键靶点之间联系密切;GO分析显示,18个关键靶点与42个生物学过程(BP)相关,与8个细胞定位(CC)相关,与9个分子功能(MF)相关;KEGG通路分析关键靶点与11条信号通路相关,与癌症通路关系最为密切。qRT-PCR检测结果显示,卵巢癌患者PTEN、MAPK14、CCNB1、CDK1、EGFR mRNA表达水平均显著升高。结论益母草可能是通过对PTEN、MAPK14、CCNB1、CDK1、EGFR的调控干预卵巢癌。展开更多
It is extremely difficult to supervise the whole production process of livestock products by traditional methods due to the long production chains. Therefore, the workflow of livestock product production were analyzed...It is extremely difficult to supervise the whole production process of livestock products by traditional methods due to the long production chains. Therefore, the workflow of livestock product production were analyzed and studied. Subsequently, information technology and modem identification techniques were used together to construct traceability system for livestock products including identifiers and information management systems. The traceability system better overcame the main constraints in application and provided a viable platform for safe production of livestock products.展开更多
The traceability management system for pigs based on personal digital assistant （PDA） was constructed by software engineering method to provide the traceability management for pork safety. This traceability system i...The traceability management system for pigs based on personal digital assistant （PDA） was constructed by software engineering method to provide the traceability management for pork safety. This traceability system included information managements for pig breeding and pig quaran- tine inspection supervision. It also realized the record and supervision of pig information, feed usage, veterinary drug usage and quarantine inspec- tion. We mainly introduced the designs of systemic structure and functional structure of the traceability system and key techniques of system imple- mentation.展开更多
文摘目的基于生物信息学揭示益母草治疗卵巢癌的分子机制。方法本研究采用GEO数据库GPL96平台共3个基因芯片,和GeneCards数据库做交集靶点,作为卵巢癌的核心疾病靶点。中药靶点的获取采用中药系统药理学分析平台(Traditional Chinese medicine system platform,TCMSP),依据口服利用度(Oral bioavailability,OB)≥30%,类药性(Drug-likeness,DL)≥0.18为益母草活性成分的筛选条件,将益母草中活性较高的成分筛选出来,并获取相应靶点蛋白。将卵巢癌的核心疾病靶点和益母草的靶点做线上Venn交集,将得到的共同靶点做生存分析,排除P>0.05的靶点,得到关键靶点;做关键靶点蛋白PPI互作网络图,DAVID数据库对关键靶点进行GO基因功能分析、KEGG通路分析。选取湖北中医药大学附属医院2018年1月—2020年1月手术治疗的60例卵巢组织标本,其中卵巢癌组织30例,正常卵巢组织30例,采用qRT-PCR法检测两组患者差异基因的表达变化。结果GeneCards数据库共记录卵巢癌6657个疾病靶点,与GPL96平台上基因芯片得到的疾病靶点进行比较,共得到487个卵巢癌疾病靶点。TCMSP数据库共记录益母草共204个靶点,与卵巢癌核心靶点做线上Venn分析,共得到22个共同靶点,将得到的共同靶点导入Kaplan Meier-Plotter进行在线生存分析,排除P>0.05的4个靶点,得到18个关键靶点;关键靶点PPI蛋白互作网络图显示,关键靶点之间联系密切;GO分析显示,18个关键靶点与42个生物学过程(BP)相关,与8个细胞定位(CC)相关,与9个分子功能(MF)相关;KEGG通路分析关键靶点与11条信号通路相关,与癌症通路关系最为密切。qRT-PCR检测结果显示,卵巢癌患者PTEN、MAPK14、CCNB1、CDK1、EGFR mRNA表达水平均显著升高。结论益母草可能是通过对PTEN、MAPK14、CCNB1、CDK1、EGFR的调控干预卵巢癌。
基金supported by grants from subtopic of National Key Technology R&D Program (2006BAD14B04)Agricultural Science and Technology Innovation Project of Yunnan Province (2008LA020)
文摘It is extremely difficult to supervise the whole production process of livestock products by traditional methods due to the long production chains. Therefore, the workflow of livestock product production were analyzed and studied. Subsequently, information technology and modem identification techniques were used together to construct traceability system for livestock products including identifiers and information management systems. The traceability system better overcame the main constraints in application and provided a viable platform for safe production of livestock products.
基金supported by the Agricultural Science and Technology Innovation Project of Yunnan Province(2008LA020)sub-topic of National Key Technology R&D Program(2006BAD14B04)
文摘The traceability management system for pigs based on personal digital assistant （PDA） was constructed by software engineering method to provide the traceability management for pork safety. This traceability system included information managements for pig breeding and pig quaran- tine inspection supervision. It also realized the record and supervision of pig information, feed usage, veterinary drug usage and quarantine inspec- tion. We mainly introduced the designs of systemic structure and functional structure of the traceability system and key techniques of system imple- mentation.
