目的:探讨超高龄髋部骨折术后患者预后情况及1年内死亡的相关危险因素。方法:回顾性分析2015年1月至2023年1月接受髋关节置换术或股骨近端髓内钉固定术治疗的332例90岁以上股骨颈骨折或转子间骨折患者的临床资料,其中男128例,女204例;年...目的:探讨超高龄髋部骨折术后患者预后情况及1年内死亡的相关危险因素。方法:回顾性分析2015年1月至2023年1月接受髋关节置换术或股骨近端髓内钉固定术治疗的332例90岁以上股骨颈骨折或转子间骨折患者的临床资料,其中男128例,女204例;年龄90~103(92.2±2.5)岁;股骨颈骨折90例,股骨转子间骨折242例。术后1年内死亡92例,分析年龄、性别、身体质量指数、骨折类型、手术方法、手术时机、手术时间、术前血红蛋白和血清白蛋白水平、受伤前行走能力、麻醉方式、伴有的基础疾病以及国际麻醉医师协会(American Society of Anesthesiology,ASA)分级等与死亡的相关性,采用单因素分析筛选出术后1年内死亡的危险因素,再将有统计学意义的危险因素纳入多因素Logostic回归分析,筛选出独立危险因素。结果:90岁及以上超高龄髋部骨折患者术后1年内死亡人数占27.7%(92/332),单因素分析结果显示死亡组身体质量指数高,伤后至手术时间间隔长,术前血清白蛋白水平低,受伤前不可独立行走,伴有肺部感染、阻塞性肺疾病、脑卒中以及心功能分级为C-D级、ASA分级Ⅲ-Ⅳ级的比例较高。多因素Logistic回归分析结果发现术前血清白蛋白<30 g·L-1[OR=2.973,95%CI(2.461,5.344),P=0.039]、伤前不可独立行走[OR=3.519,95%CI(2.224,5.413),P=0.018]、心功能分级为C-D级[OR=4.213,95%CI(2.952,6.993),P=0.021]、肺部感染[OR=3.927,95%CI(2.187,7.731),P=0.016]以及ASA分级为Ⅲ-Ⅳ级[OR=5.124,95%CI(3.092,8.235),P=0.032]是术后1年内死亡的独立危险因素。结论:术前血清白蛋白水平<30 g·L-1、伤前不能独立行走、心功能分级为C-D级、肺部感染以及ASA分级Ⅲ-Ⅳ级是90岁及以上超高龄髋部骨折术后患者术后1年内死亡的独立危险因素。展开更多
探讨铵态氮素对甘薯块根形成的调控效应及其作用机制,本研究利用单株结薯数差异显著的甘薯品种商薯19(S19)和济徐23(J23)为材料,于2014-2015年进行大田试验和盆栽辅助试验,设置60 kg hm^–2低氮(LN60)和180 kg hm^–2高氮(HN180)2个氮...探讨铵态氮素对甘薯块根形成的调控效应及其作用机制,本研究利用单株结薯数差异显著的甘薯品种商薯19(S19)和济徐23(J23)为材料,于2014-2015年进行大田试验和盆栽辅助试验,设置60 kg hm^–2低氮(LN60)和180 kg hm^–2高氮(HN180)2个氮素水平,酰胺态(XN)和铵态(AN)2种氮素形态,进行甘薯幼根向块根分化关键时期的发育解剖观察和IbEXP1基因的表达分析。结果表明,单株有效结薯数较高的商薯19块根产量显著高于济徐23,生长前期甘薯块根的分化建成相较于块根的膨大生长更利于最终块根产量的形成。同时,2个甘薯品种60 kg hm^–2铵态氮素处理在茎叶封垄期建成更多根径介于0.5~5.0 cm的薯块,显著提高了收获期的单株有效薯块数目,块根产量最高。其中,60 kg hm^–2铵态氮素处理2个品种甘薯幼根在前形成层时期原生木质部束的导管数目及中柱薄壁组织木质化薄壁细胞数目最多;初生形成层发育时期IbEXP1基因高水平表达,幼根直径、中柱直径大,原生、次生木质部束数目多;次生形成层活动初期IbEXP1基因的相对表达和中柱薄壁细胞的木质化程度介于不施氮和高氮素处理之间,但幼根直径、中柱直径和中柱占横截面比均显著最高,在块根分化建成中建立了中柱薄壁细胞木质化活动和分裂活动的平衡。展开更多
Sweetpotato, Ipomoea batatas（L.） Lam., is an important food crop widely cultivated in the world. Evaluation of genetic relationships among diverse cultivars and landraces is necessary for efficient exploitation of g...Sweetpotato, Ipomoea batatas（L.） Lam., is an important food crop widely cultivated in the world. Evaluation of genetic relationships among diverse cultivars and landraces is necessary for efficient exploitation of genetic diversity in the existing germplasm resources. In the present study, a collection of 380 sweetpotato accessions assembled from different agro-climatic zones of China and other countries were genotyped using 30 SSR primer pairs. Model-based structure analysis separated the germplasm into three populations, P1, P2 and P3, containing 228, 133 and 19 accessions, respectively, which was consistent with the results of phylogenic and principal component analysis（PCA）. Analysis of molecular variance（AMOVA） revealed significant genetic differentiation among inferred populations, accounting for 16.47% of the total molecular variance, however, the differences between the regions were not significant, the total variation were due to the differences between the genotypes within the population. Pairwise fixation index（F ST） suggested that populations P1 and P3 had the highest differentiation, while populations P1 and P2 had the lowest differentiation. The diversity among populations was wide, which confirmed the genetic distinction of populations. Through comparing model-based structure and domestication-based classification, it was found that the accessions of population P1 mainly belonged to modern cultivars, and the accessions of populations P2 and P3 basically corresponded to landraces, by which we suggest that modern cultivars maybe had experienced a two-step domestication history. Our results illustrated clear genetic relationships among 380 sweetpotato accessions, exhibiting the potential of accelerating the process of future sweetpotato breeding program by molecular marker based parental selection.展开更多
文摘目的:探讨超高龄髋部骨折术后患者预后情况及1年内死亡的相关危险因素。方法:回顾性分析2015年1月至2023年1月接受髋关节置换术或股骨近端髓内钉固定术治疗的332例90岁以上股骨颈骨折或转子间骨折患者的临床资料,其中男128例,女204例;年龄90~103(92.2±2.5)岁;股骨颈骨折90例,股骨转子间骨折242例。术后1年内死亡92例,分析年龄、性别、身体质量指数、骨折类型、手术方法、手术时机、手术时间、术前血红蛋白和血清白蛋白水平、受伤前行走能力、麻醉方式、伴有的基础疾病以及国际麻醉医师协会(American Society of Anesthesiology,ASA)分级等与死亡的相关性,采用单因素分析筛选出术后1年内死亡的危险因素,再将有统计学意义的危险因素纳入多因素Logostic回归分析,筛选出独立危险因素。结果:90岁及以上超高龄髋部骨折患者术后1年内死亡人数占27.7%(92/332),单因素分析结果显示死亡组身体质量指数高,伤后至手术时间间隔长,术前血清白蛋白水平低,受伤前不可独立行走,伴有肺部感染、阻塞性肺疾病、脑卒中以及心功能分级为C-D级、ASA分级Ⅲ-Ⅳ级的比例较高。多因素Logistic回归分析结果发现术前血清白蛋白<30 g·L-1[OR=2.973,95%CI(2.461,5.344),P=0.039]、伤前不可独立行走[OR=3.519,95%CI(2.224,5.413),P=0.018]、心功能分级为C-D级[OR=4.213,95%CI(2.952,6.993),P=0.021]、肺部感染[OR=3.927,95%CI(2.187,7.731),P=0.016]以及ASA分级为Ⅲ-Ⅳ级[OR=5.124,95%CI(3.092,8.235),P=0.032]是术后1年内死亡的独立危险因素。结论:术前血清白蛋白水平<30 g·L-1、伤前不能独立行走、心功能分级为C-D级、肺部感染以及ASA分级Ⅲ-Ⅳ级是90岁及以上超高龄髋部骨折术后患者术后1年内死亡的独立危险因素。
文摘探讨铵态氮素对甘薯块根形成的调控效应及其作用机制,本研究利用单株结薯数差异显著的甘薯品种商薯19(S19)和济徐23(J23)为材料,于2014-2015年进行大田试验和盆栽辅助试验,设置60 kg hm^–2低氮(LN60)和180 kg hm^–2高氮(HN180)2个氮素水平,酰胺态(XN)和铵态(AN)2种氮素形态,进行甘薯幼根向块根分化关键时期的发育解剖观察和IbEXP1基因的表达分析。结果表明,单株有效结薯数较高的商薯19块根产量显著高于济徐23,生长前期甘薯块根的分化建成相较于块根的膨大生长更利于最终块根产量的形成。同时,2个甘薯品种60 kg hm^–2铵态氮素处理在茎叶封垄期建成更多根径介于0.5~5.0 cm的薯块,显著提高了收获期的单株有效薯块数目,块根产量最高。其中,60 kg hm^–2铵态氮素处理2个品种甘薯幼根在前形成层时期原生木质部束的导管数目及中柱薄壁组织木质化薄壁细胞数目最多;初生形成层发育时期IbEXP1基因高水平表达,幼根直径、中柱直径大,原生、次生木质部束数目多;次生形成层活动初期IbEXP1基因的相对表达和中柱薄壁细胞的木质化程度介于不施氮和高氮素处理之间,但幼根直径、中柱直径和中柱占横截面比均显著最高,在块根分化建成中建立了中柱薄壁细胞木质化活动和分裂活动的平衡。
基金supported by the Hubei Engineering and Technology Research Centre of Sweetpotato,Chinathe International Cooperation Program,Ministry of Science and Technology of China (2011DFB31620)+3 种基金the Introduction and Utiliztion of Sweetpotato Resources in USA (2013-Z61)the Characteristic Discipline of Hubei Academy of Agricultural Sciences,Chinathe Science and Technology Innovation Centre of Hubei Academy of Agricultural Sciences,China (2007-620-001-03)the China Agriculture Research System (CARS-11-C-15)
文摘Sweetpotato, Ipomoea batatas（L.） Lam., is an important food crop widely cultivated in the world. Evaluation of genetic relationships among diverse cultivars and landraces is necessary for efficient exploitation of genetic diversity in the existing germplasm resources. In the present study, a collection of 380 sweetpotato accessions assembled from different agro-climatic zones of China and other countries were genotyped using 30 SSR primer pairs. Model-based structure analysis separated the germplasm into three populations, P1, P2 and P3, containing 228, 133 and 19 accessions, respectively, which was consistent with the results of phylogenic and principal component analysis（PCA）. Analysis of molecular variance（AMOVA） revealed significant genetic differentiation among inferred populations, accounting for 16.47% of the total molecular variance, however, the differences between the regions were not significant, the total variation were due to the differences between the genotypes within the population. Pairwise fixation index（F ST） suggested that populations P1 and P3 had the highest differentiation, while populations P1 and P2 had the lowest differentiation. The diversity among populations was wide, which confirmed the genetic distinction of populations. Through comparing model-based structure and domestication-based classification, it was found that the accessions of population P1 mainly belonged to modern cultivars, and the accessions of populations P2 and P3 basically corresponded to landraces, by which we suggest that modern cultivars maybe had experienced a two-step domestication history. Our results illustrated clear genetic relationships among 380 sweetpotato accessions, exhibiting the potential of accelerating the process of future sweetpotato breeding program by molecular marker based parental selection.
