The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2–3 times faster than females. Therefore, all-male breeding could increase production of this fish, a...The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2–3 times faster than females. Therefore, all-male breeding could increase production of this fish, and sex-linked markers would be useful during the breeding process. In this study, a male-associated marker named PuGT54 was isolated in P. ussuriensis from a previously constructed microsatellite library. A total of 185 P. ussuriensis individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified, indicating the high reliability and accuracy of this marker for sex identifi cation. Furthermore, the male-specifi c fragment amplifi ed by this maker was cloned and sequenced, which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn, detailed information of the male-specifi c fragment is unknown presently. Nevertheless, this marker would be helpful in conservation, breeding and ecological study of P. ussuriensis.展开更多
遵循“基于自然的解决方案(Natural based solution)”理念,本研究开发了由渗滤型边滩、生态跌水和近自然河溪构成的河流多生境自然塑造技术及系统,并在北运河流域的再生水河道台头沟实施了工程应用与示范.结果表明,该技术与系统具有良...遵循“基于自然的解决方案(Natural based solution)”理念,本研究开发了由渗滤型边滩、生态跌水和近自然河溪构成的河流多生境自然塑造技术及系统,并在北运河流域的再生水河道台头沟实施了工程应用与示范.结果表明,该技术与系统具有良好的水质净化效果,对氨氮、总氮、总磷和化学需氧量的平均削减率分别为62.15%、47.46%、48.50%和70.84%;河道修复使得生境质量得到改善,由D级(差)提升至B级(良),生境类型由不足3种提升至超过8种;修复后河道底栖动物的种类数量、BI指数、多样性指数等增加,水鸟从无到有,生物多样性也逐步改善.该技术可为城市特别是城乡结合部小型再生水河道治理提供借鉴.展开更多
基金Supported by the National Natural Science Foundation of China(No.31602146)the Scientific Funds of Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection(No.HSXT307)+1 种基金the Start-up Funds of Scientifi c Research of Huaiyin Normal University(No.31ZCK00)the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP)
文摘The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2–3 times faster than females. Therefore, all-male breeding could increase production of this fish, and sex-linked markers would be useful during the breeding process. In this study, a male-associated marker named PuGT54 was isolated in P. ussuriensis from a previously constructed microsatellite library. A total of 185 P. ussuriensis individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified, indicating the high reliability and accuracy of this marker for sex identifi cation. Furthermore, the male-specifi c fragment amplifi ed by this maker was cloned and sequenced, which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn, detailed information of the male-specifi c fragment is unknown presently. Nevertheless, this marker would be helpful in conservation, breeding and ecological study of P. ussuriensis.
文摘遵循“基于自然的解决方案(Natural based solution)”理念,本研究开发了由渗滤型边滩、生态跌水和近自然河溪构成的河流多生境自然塑造技术及系统,并在北运河流域的再生水河道台头沟实施了工程应用与示范.结果表明,该技术与系统具有良好的水质净化效果,对氨氮、总氮、总磷和化学需氧量的平均削减率分别为62.15%、47.46%、48.50%和70.84%;河道修复使得生境质量得到改善,由D级(差)提升至B级(良),生境类型由不足3种提升至超过8种;修复后河道底栖动物的种类数量、BI指数、多样性指数等增加,水鸟从无到有,生物多样性也逐步改善.该技术可为城市特别是城乡结合部小型再生水河道治理提供借鉴.
