目的评价天智颗粒联合西药治疗血管性痴呆的有效性和安全性。方法计算机检索中国期刊网(CNKI)、万方数据库(Wanfang)、维普数据库(VIP)、中国生物医学文献数据库(SINOMED)等中文数据库及PubMed、Embase、MEDLINE、Cochrane Library等外...目的评价天智颗粒联合西药治疗血管性痴呆的有效性和安全性。方法计算机检索中国期刊网(CNKI)、万方数据库(Wanfang)、维普数据库(VIP)、中国生物医学文献数据库(SINOMED)等中文数据库及PubMed、Embase、MEDLINE、Cochrane Library等外文数据库,收集天智颗粒治疗血管性痴呆的随机对照临床试验(RCT)文献,检索时限为数据库建库至2019年5月31日。根据纳入标准、排除标准筛选和提取文献资料,采用Rev Man 5.3软件进行Meta分析。结果共纳入12篇RCT文献,共992例患者,其中天智颗粒联合西药治疗组(治疗组)507例,西医常规治疗组(对照组)485例。Meta分析结果显示,治疗组总有效率优于对照组[OR=2.58,95%C(I1.81,3.69),P<0.00001];天智颗粒治疗组能升高简易智力状态检查量表(MMSE)评分[MD=1.15;95%CI(0.77,1.53);P<0.00001]。对于日常生活活动能力量表(ADL)评分,两组疗效相当[MD=-2.00,95%CI(-4.13,0.14),P=0.07]。天智颗粒治疗血管性痴呆未见明显不良反应。结论天智颗粒能有效提高血管性痴呆临床疗效,但所纳入文献的质量不高,样本量较少,亟待开展多中心、大样本、双盲的RCT,以评价天智颗粒的长期疗效及安全性。展开更多
METHODS:In the present study,female SpragueDawley rats were randomly divided into four groups with 6 of each,including normal control group(NC),jumpinginduced muscle injury model group(JI),JI with electroacupuncture s...METHODS:In the present study,female SpragueDawley rats were randomly divided into four groups with 6 of each,including normal control group(NC),jumpinginduced muscle injury model group(JI),JI with electroacupuncture stimulation treatment group(EA),and JI with non-electroacupuncture stimulation group(NEA).Transmission electron microscopy,transcriptome sequencing and analysis,prediction of protein interaction networks,real-time polymerase chain reaction verification,and Western blotting were performed on the gastrocnemius muscle of ipsilateral lower limbs.RESULTS:The structural repair of injured gastrocnemius myofibers following jumping training in EA rats was better than that of NEA rats.A total of 136 genes were differentially expressed in EA rats relative to JI rats,with 55 genes upregulated and 81 genes downregulated.According to results of transcriptome analysis,and prediction of protein mutual interaction by the online STRING database,Heat shock protein beta-7(Hspb7)and myozenin2(Myoz2)genes were targeted.Expressions of Hspb7 and Myoz2 mRNAs were increased in EA rats relative to JI rats(P<0.05).The expression of Hspb7 protein was upregulated in EA rats relative to that in NC,JI,and NEA rats(P<0.01,<0.05,and<0.05,respectively).The expression of Myoz2 protein was upregulated in EA rats relative to that in NC and JI rats(both P<0.01,respectively).CONCLUSIONS:The present results suggest that electroacupuncture stimulation at Zusanli(ST36)could improve muscle healing following jumping-induced muscle injury,owing to the upregulation of Hspb7 and Myoz2 proteins.展开更多
文摘目的评价天智颗粒联合西药治疗血管性痴呆的有效性和安全性。方法计算机检索中国期刊网(CNKI)、万方数据库(Wanfang)、维普数据库(VIP)、中国生物医学文献数据库(SINOMED)等中文数据库及PubMed、Embase、MEDLINE、Cochrane Library等外文数据库,收集天智颗粒治疗血管性痴呆的随机对照临床试验(RCT)文献,检索时限为数据库建库至2019年5月31日。根据纳入标准、排除标准筛选和提取文献资料,采用Rev Man 5.3软件进行Meta分析。结果共纳入12篇RCT文献,共992例患者,其中天智颗粒联合西药治疗组(治疗组)507例,西医常规治疗组(对照组)485例。Meta分析结果显示,治疗组总有效率优于对照组[OR=2.58,95%C(I1.81,3.69),P<0.00001];天智颗粒治疗组能升高简易智力状态检查量表(MMSE)评分[MD=1.15;95%CI(0.77,1.53);P<0.00001]。对于日常生活活动能力量表(ADL)评分,两组疗效相当[MD=-2.00,95%CI(-4.13,0.14),P=0.07]。天智颗粒治疗血管性痴呆未见明显不良反应。结论天智颗粒能有效提高血管性痴呆临床疗效,但所纳入文献的质量不高,样本量较少,亟待开展多中心、大样本、双盲的RCT,以评价天智颗粒的长期疗效及安全性。
基金Supported by Science and Technology Department of Henan Province Effect Mechanisms of Acupuncture to Promote the Repair of Injured Skeletal Muscle(No.212102310260)Study on the Relationship between Hspb7 Protein and JAK/STAT Pathway and Electroacupuncture Intervention in Muscle Injury based on mRNA-Seq Analysis(No.222102320072)Science and Technology Bureau o Kaifeng:the Effect of Platelet Plasma Injection on Patellar Tendon Fibrosis(No.2003006)。
文摘METHODS:In the present study,female SpragueDawley rats were randomly divided into four groups with 6 of each,including normal control group(NC),jumpinginduced muscle injury model group(JI),JI with electroacupuncture stimulation treatment group(EA),and JI with non-electroacupuncture stimulation group(NEA).Transmission electron microscopy,transcriptome sequencing and analysis,prediction of protein interaction networks,real-time polymerase chain reaction verification,and Western blotting were performed on the gastrocnemius muscle of ipsilateral lower limbs.RESULTS:The structural repair of injured gastrocnemius myofibers following jumping training in EA rats was better than that of NEA rats.A total of 136 genes were differentially expressed in EA rats relative to JI rats,with 55 genes upregulated and 81 genes downregulated.According to results of transcriptome analysis,and prediction of protein mutual interaction by the online STRING database,Heat shock protein beta-7(Hspb7)and myozenin2(Myoz2)genes were targeted.Expressions of Hspb7 and Myoz2 mRNAs were increased in EA rats relative to JI rats(P<0.05).The expression of Hspb7 protein was upregulated in EA rats relative to that in NC,JI,and NEA rats(P<0.01,<0.05,and<0.05,respectively).The expression of Myoz2 protein was upregulated in EA rats relative to that in NC and JI rats(both P<0.01,respectively).CONCLUSIONS:The present results suggest that electroacupuncture stimulation at Zusanli(ST36)could improve muscle healing following jumping-induced muscle injury,owing to the upregulation of Hspb7 and Myoz2 proteins.
