Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid biopsy.Aptamer is considered as a promising molecular probe for exosomes detecti...Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid biopsy.Aptamer is considered as a promising molecular probe for exosomes detection because of the high binding affinity,remarkable specificity,and low cost.Recently,many approaches have been developed to further improve the performance of electrochemical aptamer based(E-AB)sensors with a lower limit of detection.In this review,we focus on the development of using aptamer as a specific recognition element for exosomes detection in electrochemical sensors.We first introduce recent advances in evolving aptamers against exosomes.Then,we review methods of immobilization aptamers on electrode surfaces,followed by a summary of the main strategies of signal amplification.Finally,we present the insights of the challenges and future directions of E-AB sensors for exosomes analysis.展开更多
基金supported by the National Natural Science Foundation of China(Nos.22004134,21804146)the Fundamental Research Funds for the Central Universities,South-Central Minzu University,China(Nos.CZY20006,CZQ21025).
文摘Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid biopsy.Aptamer is considered as a promising molecular probe for exosomes detection because of the high binding affinity,remarkable specificity,and low cost.Recently,many approaches have been developed to further improve the performance of electrochemical aptamer based(E-AB)sensors with a lower limit of detection.In this review,we focus on the development of using aptamer as a specific recognition element for exosomes detection in electrochemical sensors.We first introduce recent advances in evolving aptamers against exosomes.Then,we review methods of immobilization aptamers on electrode surfaces,followed by a summary of the main strategies of signal amplification.Finally,we present the insights of the challenges and future directions of E-AB sensors for exosomes analysis.
