巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发...巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。展开更多
Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and resi...Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines(RHL) derived from the cross between Yangyandou(low level of β subunit) and Zhonghuang 13(normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1(β subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome(Glyma 1.0, Williams 82), including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine(TT) insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection(MAS).展开更多
Developing expressed sequence tag-derived SSR(EST-SSR)markers is imperative in genetic research.In this paper,we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library.Am...Developing expressed sequence tag-derived SSR(EST-SSR)markers is imperative in genetic research.In this paper,we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library.Among the 286 markers designed for the 4 accessions of Glycine max and 6 of its wild progenitor(G.soja)within the subgenus Soja,209 markers amplified DNA fragments,taking 73.1% and 37 markers appeared to be polymorphic,which was 12.9% of the total.The 37 loci detected a total of 142 alleles,while the PIC values varied from 0.194 to 0.794.Both the number of alleles per locus and PIC value were significantly related to the SSR motif.Six EST-SSR loci may be fixed for different alleles between G.max and G.soja since they were particularly polymorphic among the 6 G.soja accessions.A neighbor-joining tree placed the G.max accessions together as a group within the G.soja,though the average genetic distance among G.soja accessions was much higher.These new EST-SSRs markers will be useful for genetic diversity analysis,genetic mapping construction and gene discovery in Soja subgenus.展开更多
The growth periods(GPs, from planting/emergence to reproductive stage 8(R8) of soybean cultivars vary in different ecological regions, especially in China with a very complex soybean cropping system. In this study, a ...The growth periods(GPs, from planting/emergence to reproductive stage 8(R8) of soybean cultivars vary in different ecological regions, especially in China with a very complex soybean cropping system. In this study, a 3-yr experimental study was undertaken in three geographical locations of China from 2008 to 2010, including the Northeast(40.66–45.85°N), Huang-Huai(34.75–38.04°N) and southern(22.82–30.60°N) eco-regions with about 250 accessions in each region to clarify the classification of maturity group(MG) and identify the cultivars with stable GP to increase the knowledge about the GP distribution of soybean cultivars in China. GPs of soybean cultivars in different eco-regions were significant different with a gradual decrease from 115–125 d in the Northeast part to the 85–100 d in the southern part of China. The geographical location was the major factor for GP of cultivars from the Northeast, while the year of planting was the major factor affecting the stability of GPs in Huang-Huai summer and southern summer soybean. AMMI2(additive main effects and multiplicativeinteraction)-Biplot analysis showed that the GPs of soybean cultivars from the Northeast eco-region have a comparatively satisfactory environmental stability. Moreover, soybean cultivars with moderate GP/MG and stable environment adaptability in different eco-regions were identified based on the linear regression and AMMI analysis, which was important for the accurate classification of soybean MGs in future. Taken together, our results reflected the genetic diversity, geographical distribution and environmental stability of the Chinese soybean GP trait. Soybean cultivars with stable GP for various Chinese eco-regions would be beneficial for Chinese soybean genetic improvement, varietal introduction, exchange, and soybean breeding program for wide adaptability.展开更多
Information on the center of genetic diversity of soybean(Glycine max) will be helpful not only for designing efficient strategies for breeding programs, but also for understanding the domestication and origin of this...Information on the center of genetic diversity of soybean(Glycine max) will be helpful not only for designing efficient strategies for breeding programs, but also for understanding the domestication and origin of this species. Here, we describe an analysis of genetic diversity based on simple-sequence repeat(SSR) variations within a core collection of 2 111 accessions of Chinese soybean landraces. Prior to the diversity assessment, the geographic origin of each accession was mapped. The map was then divided into grids each 2.5° in latitude and 5° in longitude. We found two regions that had higher number of alleles(NA) and greater polymorphic information content(PIC) values than the others. These regions are adjacently located within grid position of 30°–35°N×105°–110°E, which includes the valley of the middle and lower reaches of the Wei River, and the valley of the upper reaches of the Hanjiang River. It was also observed that in many regions, genetic diversity decreased with the increase in distance from the center. Another region, in northern Hebei Province(115°–120°E×40°–42.5°N), was observed having higher diversity than any surrounding regions, indicating that this is a sub-center of soybean diversity. Based on the presented results, the domestication and origin of soybean are also discussed.展开更多
For clarifying the hierarchical patterns of population structure of soybean landraces in China, the seven clusters previously identified using Bayesian clustering of 1 504 soybean landraces based on SSR markers genoty...For clarifying the hierarchical patterns of population structure of soybean landraces in China, the seven clusters previously identified using Bayesian clustering of 1 504 soybean landraces based on SSR markers genotyping data were further analyzed. Using the largest value of ΔK, these landraces could be split into 20 sub-clusters, which was supported by highly significant pairwise Fst-values and generally in accordance with the geographic origin and sowing types. The autumn-sowing types ended up in one distinct sub-cluster from the otherwise summer-sowing type, where the autumn-sowing types are most likely derived from. The division into 20 sub-clusters explained 7.3% of the genetic variation, next to 9.7% present among the seven clusters, 81.1% residing among landraces within sub-clusters, and 1.9% within the landraces. The distribution pattern of genetic diversity among the sub-clusters of each cluster was uneven, with two HSuM sub-clusters (Central China) and some South China sub-clusters showing significantly higher level of genetic diversity.展开更多
The cultivated soybean(Glycine max(L.) Merr.) was distinguished from its wild progenitor Glycine soja Sieb.& Zucc.in growth period structure,by a shorter vegetative phase(V),a prolonged reproductive phase(R) and h...The cultivated soybean(Glycine max(L.) Merr.) was distinguished from its wild progenitor Glycine soja Sieb.& Zucc.in growth period structure,by a shorter vegetative phase(V),a prolonged reproductive phase(R) and hence a larger R/V ratio.However,the genetic basis of the domestication of soybean from wild materials is unclear.Here,a panel of 123 cultivated and 97 wild accessions were genotyped using a set of 24 presence/absence variants(PAVs) while at the same time the materials were phenotyped with respect to flowering and maturity times at two trial sites located at very different latitudes.The major result of this study showed that variation at PAVs is informative for assessing patterns of genetic diversity in Glycine spp.The genotyping was largely consistent with the taxonomic status,although a few accessions were intermediate between the two major clades identified.Allelic diversity was much higher in the wild germplasm than in the cultivated materials.A significant domestication signal was detected at 11 of the PAVs at 0.01 level.In particular,this study has provided information for revealing the genetic basis of photoperiodism which was a prominent feature for the domestication of soybean.A significant marker-trait association with R/V ratio was detected at 14 of the PAVs,but stripping out population structure reduced this to three.These results will provide markers information for further finding of R/V related genes that can help to understand the domestication process and introgress novel genes in wild soybean to broaden the genetic base of modern soybean cultivars.展开更多
文摘巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。
基金funded by the National High-Tech R&D Program of China(2012AA101106)the National Basic Research Program of China(2009CB118404)+1 种基金the Key Technologies R&D Program of China during the 12th Five-Year Plan period(2011BAD35B06)the National Transgenic Major Program,China(2008ZX08009-003)
文摘Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great significance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines(RHL) derived from the cross between Yangyandou(low level of β subunit) and Zhonghuang 13(normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus, qBSC-1(β subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes within qBSC-1 region based on the reference genome(Glyma 1.0, Williams 82), including the two copies of β subunit coding gene CG4. An InDel marker developed from a thymine(TT) insertion in one copy of CG4 promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 within qBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection(MAS).
基金supported by the National High-Tech R&D Program of China(863Program,2006AA10A110 and 2006AA10Z164)National Basic Research Program of China(973 Program,2010CB125900 and 2004CB117203)the Academy and Institute Foundation for Basic Scientific Research in Institute of Crop Science,Chinese Academy of Agricultural Sciences
文摘Developing expressed sequence tag-derived SSR(EST-SSR)markers is imperative in genetic research.In this paper,we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library.Among the 286 markers designed for the 4 accessions of Glycine max and 6 of its wild progenitor(G.soja)within the subgenus Soja,209 markers amplified DNA fragments,taking 73.1% and 37 markers appeared to be polymorphic,which was 12.9% of the total.The 37 loci detected a total of 142 alleles,while the PIC values varied from 0.194 to 0.794.Both the number of alleles per locus and PIC value were significantly related to the SSR motif.Six EST-SSR loci may be fixed for different alleles between G.max and G.soja since they were particularly polymorphic among the 6 G.soja accessions.A neighbor-joining tree placed the G.max accessions together as a group within the G.soja,though the average genetic distance among G.soja accessions was much higher.These new EST-SSRs markers will be useful for genetic diversity analysis,genetic mapping construction and gene discovery in Soja subgenus.
基金supported by the Program of Accurate Identification and Display of Soybean Germplasm, China (NB08-2130315-(25-31)-06, NB07-2130315-(25-30)-06, NB06-070401-(22-27)-05), NB2010-2130315-25-05)the National Crop Germplasm Platform, Ministry of Agriculture and Ministry of Science and Technology, China (2012-004, 2014-004)
文摘The growth periods(GPs, from planting/emergence to reproductive stage 8(R8) of soybean cultivars vary in different ecological regions, especially in China with a very complex soybean cropping system. In this study, a 3-yr experimental study was undertaken in three geographical locations of China from 2008 to 2010, including the Northeast(40.66–45.85°N), Huang-Huai(34.75–38.04°N) and southern(22.82–30.60°N) eco-regions with about 250 accessions in each region to clarify the classification of maturity group(MG) and identify the cultivars with stable GP to increase the knowledge about the GP distribution of soybean cultivars in China. GPs of soybean cultivars in different eco-regions were significant different with a gradual decrease from 115–125 d in the Northeast part to the 85–100 d in the southern part of China. The geographical location was the major factor for GP of cultivars from the Northeast, while the year of planting was the major factor affecting the stability of GPs in Huang-Huai summer and southern summer soybean. AMMI2(additive main effects and multiplicativeinteraction)-Biplot analysis showed that the GPs of soybean cultivars from the Northeast eco-region have a comparatively satisfactory environmental stability. Moreover, soybean cultivars with moderate GP/MG and stable environment adaptability in different eco-regions were identified based on the linear regression and AMMI analysis, which was important for the accurate classification of soybean MGs in future. Taken together, our results reflected the genetic diversity, geographical distribution and environmental stability of the Chinese soybean GP trait. Soybean cultivars with stable GP for various Chinese eco-regions would be beneficial for Chinese soybean genetic improvement, varietal introduction, exchange, and soybean breeding program for wide adaptability.
基金supported by the National Basic Research Program of China(973,G1998010203 and 2004CB117203)the Agricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences
文摘Information on the center of genetic diversity of soybean(Glycine max) will be helpful not only for designing efficient strategies for breeding programs, but also for understanding the domestication and origin of this species. Here, we describe an analysis of genetic diversity based on simple-sequence repeat(SSR) variations within a core collection of 2 111 accessions of Chinese soybean landraces. Prior to the diversity assessment, the geographic origin of each accession was mapped. The map was then divided into grids each 2.5° in latitude and 5° in longitude. We found two regions that had higher number of alleles(NA) and greater polymorphic information content(PIC) values than the others. These regions are adjacently located within grid position of 30°–35°N×105°–110°E, which includes the valley of the middle and lower reaches of the Wei River, and the valley of the upper reaches of the Hanjiang River. It was also observed that in many regions, genetic diversity decreased with the increase in distance from the center. Another region, in northern Hebei Province(115°–120°E×40°–42.5°N), was observed having higher diversity than any surrounding regions, indicating that this is a sub-center of soybean diversity. Based on the presented results, the domestication and origin of soybean are also discussed.
基金supported by the National Basic Research Program of China (973 Program,2010CB125900 and 2004CB117203)the National Key Technologies R&D Program during the 11th Five-Year-Plan period (2006BAD13B05)+1 种基金the National Natural Science Foundation of China (30490251 and30471096)the Academy and Institute Foundation for Basic Scientific Research in Institute of Crop Sciences,Chinese Academy of Agricultural Sciences
文摘For clarifying the hierarchical patterns of population structure of soybean landraces in China, the seven clusters previously identified using Bayesian clustering of 1 504 soybean landraces based on SSR markers genotyping data were further analyzed. Using the largest value of ΔK, these landraces could be split into 20 sub-clusters, which was supported by highly significant pairwise Fst-values and generally in accordance with the geographic origin and sowing types. The autumn-sowing types ended up in one distinct sub-cluster from the otherwise summer-sowing type, where the autumn-sowing types are most likely derived from. The division into 20 sub-clusters explained 7.3% of the genetic variation, next to 9.7% present among the seven clusters, 81.1% residing among landraces within sub-clusters, and 1.9% within the landraces. The distribution pattern of genetic diversity among the sub-clusters of each cluster was uneven, with two HSuM sub-clusters (Central China) and some South China sub-clusters showing significantly higher level of genetic diversity.
基金supported by the Agricultural Science and Technology Innovation Program(ASTIP) of Chinese Academy of Agricultural Sciences and the Platform of National Crop Germplasm Resources of China(nos.2012-004 and 2013-004)
文摘The cultivated soybean(Glycine max(L.) Merr.) was distinguished from its wild progenitor Glycine soja Sieb.& Zucc.in growth period structure,by a shorter vegetative phase(V),a prolonged reproductive phase(R) and hence a larger R/V ratio.However,the genetic basis of the domestication of soybean from wild materials is unclear.Here,a panel of 123 cultivated and 97 wild accessions were genotyped using a set of 24 presence/absence variants(PAVs) while at the same time the materials were phenotyped with respect to flowering and maturity times at two trial sites located at very different latitudes.The major result of this study showed that variation at PAVs is informative for assessing patterns of genetic diversity in Glycine spp.The genotyping was largely consistent with the taxonomic status,although a few accessions were intermediate between the two major clades identified.Allelic diversity was much higher in the wild germplasm than in the cultivated materials.A significant domestication signal was detected at 11 of the PAVs at 0.01 level.In particular,this study has provided information for revealing the genetic basis of photoperiodism which was a prominent feature for the domestication of soybean.A significant marker-trait association with R/V ratio was detected at 14 of the PAVs,but stripping out population structure reduced this to three.These results will provide markers information for further finding of R/V related genes that can help to understand the domestication process and introgress novel genes in wild soybean to broaden the genetic base of modern soybean cultivars.