A novel camptothecin （CPT） prodrug was successfully synthesized by conjugating CPT to adamantanecarboxylic acid （AD） via a cleavable disulfide linkage. The resulting CPT-ss-AD prodrug could act as a low molecular ...A novel camptothecin （CPT） prodrug was successfully synthesized by conjugating CPT to adamantanecarboxylic acid （AD） via a cleavable disulfide linkage. The resulting CPT-ss-AD prodrug could act as a low molecular weight gelator to form molecular gels in water/water-miscible organic solvent mixture. Meanwhile, biode- gradable amphiphilic block copolymer mPEG-b-P （MAC-co-DTC） （PPMD） was also employed as an organic framework together with CPT-ss-AD to form gel structure. CPT-ss-AD/PPMD gel exhibited less compact molecular arrangement but much more stability than CPT-ss-AD gel. The two kinds of gels could effectively release the original CPT under reductive condition at a near-constant rate without any initial burst. As compared to CPT-ss-AD single-component gel, the two-component gel, CPT-ss-AD/PPMD, had a significantly higher release rate of CPT, while 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide （MTT） assays also indicated highly potent cytotoxic activity against HeLa cells.展开更多
基金Supported by the Natural Science Foundation of Hubei Province of China(2014CFB696)the Opening Project of Key Laboratory of Biomedical Polymers of Ministry of Education at Wuhan University(20150102)the National Natural Science Foundation of China(21074098)
文摘A novel camptothecin （CPT） prodrug was successfully synthesized by conjugating CPT to adamantanecarboxylic acid （AD） via a cleavable disulfide linkage. The resulting CPT-ss-AD prodrug could act as a low molecular weight gelator to form molecular gels in water/water-miscible organic solvent mixture. Meanwhile, biode- gradable amphiphilic block copolymer mPEG-b-P （MAC-co-DTC） （PPMD） was also employed as an organic framework together with CPT-ss-AD to form gel structure. CPT-ss-AD/PPMD gel exhibited less compact molecular arrangement but much more stability than CPT-ss-AD gel. The two kinds of gels could effectively release the original CPT under reductive condition at a near-constant rate without any initial burst. As compared to CPT-ss-AD single-component gel, the two-component gel, CPT-ss-AD/PPMD, had a significantly higher release rate of CPT, while 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide （MTT） assays also indicated highly potent cytotoxic activity against HeLa cells.
