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气相色谱-质谱法测定苹果和番茄中11种农药残留 被引量:4
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作者 晁桂梅 赵舒景 +2 位作者 陈恳 马莉 金博艳 《食品工业科技》 CAS 北大核心 2020年第16期227-231,共5页
建立气相色谱-质谱联用法分别检测苹果、番茄两种基质中11种农药残留的测定方法。样品经乙腈提取,SPE NH2柱净化,净化液浓缩,正己烷定容后,采用气相色谱-质谱联用法测定,外标法定量。结果表明,在定量限至五倍定量限浓度范围内,两种基质... 建立气相色谱-质谱联用法分别检测苹果、番茄两种基质中11种农药残留的测定方法。样品经乙腈提取,SPE NH2柱净化,净化液浓缩,正己烷定容后,采用气相色谱-质谱联用法测定,外标法定量。结果表明,在定量限至五倍定量限浓度范围内,两种基质中11种农药的线性方程相关系数大于0.998,检出限和定量限范围分别为0.0038~0.0300和0.0126~0.1000 mg/kg,3个浓度点6个平行样品的加标平均回收率在81.4%~118.3%之间,相对标准偏差低于10%。本方法具有前处理简单快捷、准确度和精密度高、检测成本相对较低等优点。 展开更多
关键词 气相色谱-质谱法 苹果 番茄 农药残留
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Genetic diversity of Ustilago hordei in Tibetan areas as revealed by RAPD and SSR 被引量:2
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作者 ZHOU Yu chao gui-mei +3 位作者 LIU Jia-jia ZHU Ming-qi WANG Yang FENG Bai-li 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第10期2299-2308,共10页
Covered smut, which is caused by Ustilago hordei(Pers.) Lagerh., is one of the most damaging diseases of highland barley(Hordeum vulgare Linn. var. nudum Hook. f) in Tibetan areas of China. To understand the molec... Covered smut, which is caused by Ustilago hordei(Pers.) Lagerh., is one of the most damaging diseases of highland barley(Hordeum vulgare Linn. var. nudum Hook. f) in Tibetan areas of China. To understand the molecular diversity of U. hordei, a total of 27 isolates, which were collected from highland barley plants from Tibet, Sichuan, Qinghai, and Gansu provinces/autonomous region, were analyzed using random amplified polymorphic DNA(RAPD) and simple sequence repeat(SSR) markers. Among the 100 RAPD primers used, 24 primers exhibited polymorphism. A total of 111 fragments were amplified, of which 103 were polymorphic with a polymorphic rate of 92.79%. The average observed number of alleles(Na), effective number of alleles(Ne), Nei's genetic diversity(H), Shannon's information index(I) and polymorphism information content(PIC) value in the RAPD markers were 1.9279, 1.5016, 0.2974, 0.4503 and 0.6428, respectively. For the SSR markers, 40 of the 111 primer pairs exhibited polymorphism and provided a total of 119 bands, of which 109 were polymorphic and accounted for 91.60% of the total bands. The Na, Ne, H, I and PIC values of the SSR markers were 1.9160, 1.4639, 0.2757, 0.4211 and 0.4340, respectively. The similarity coefficients ranged from 0.4957 to 0.9261 with an average of 0.7028 among all the 27 isolates used. The dendrogram, which was developed based on the RAPD and SSR combined marker dataset showed that the 27 U. hordei isolates were divided into 3 clusters at similarity coefficient of 0.7314. We determined that RAPD and SSR markers can be successfully used to assess the genetic variation among U. hordei isolates. The RAPD markers revealed higher levels of genetic polymorphism than did the SSR markers in this study. There existed a moderate genetic difference among isolates. The molecular variation and differentiation was somewhat associated with geographical origin but not for all of the isolates. 展开更多
关键词 highland barley Ustilago hordei RAPD SSR genetic diversity
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