Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE te...Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE technique. The sequence analysis showed the open reading frame （ORF） of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein （GFP） marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase.展开更多
Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for ea...Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F1 offspring in this research.展开更多
This paper carried on a preliminary study on pigment composition of lilies of different colors by means of specific color reactions and UV-visible spectra. The results showed that the colors of lilies were usually cau...This paper carried on a preliminary study on pigment composition of lilies of different colors by means of specific color reactions and UV-visible spectra. The results showed that the colors of lilies were usually caused by the combined action of several pigments; yellow and orange lilies mainly contained the flavonoids and carotenoids; pink lilies mainly contained fiavonoids and anthocyanins, and white lilies contained small amount of flavonoids.展开更多
Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ...Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.展开更多
基金supported by the Heilongjiang Province Postdoctoral Science Foundation(LBH-Q10144)
文摘Five different isoforms （IrlVHA-c1-c5） of V-ATPase subunit c （VHA-c） were cloned from a Japanese iris （Iris lactea Pall. var. chinensis Fisch. Koidz） cDNA library using degenerate primers PCR and the 5＇-RACE technique. The sequence analysis showed the open reading frame （ORF） of the IrlVHA-c1 c5 to be 495 bp, corresponding to a protein of 164 amino acids. Among the five isoforms, IrlVHA-c1 and IrlVHA-c2 are completely homologous. The IrlVHA-c protein is localized at the vacuolar membrane as indicated by a green fluorescent protein （GFP） marker. Its over-expression in yeast could enhance yeast tolerance to NaCl stress. These results show that there are at least five genes encoding different isoforms of IrlVHA-c in Japanese iris and IrlVHA-c is important for the function of V-ATPase.
基金Supported by the National Natural Science Foundation of China (31171986)Science and Technology Creative Talents of the Special Fund Project of Harbin (RC2012XK002013)Overseas Scholars Research Funding Project of Education Department of Heilongjiang Province (1154h04)
文摘Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F1 offspring in this research.
文摘This paper carried on a preliminary study on pigment composition of lilies of different colors by means of specific color reactions and UV-visible spectra. The results showed that the colors of lilies were usually caused by the combined action of several pigments; yellow and orange lilies mainly contained the flavonoids and carotenoids; pink lilies mainly contained fiavonoids and anthocyanins, and white lilies contained small amount of flavonoids.
基金Supported by Heilongjiang Province Science and Technology Research Projects (GB06B112-5)
文摘Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.
