Marker Assisted Selection in Citrus Rootstock Breeding Based on a Major Gene Locus ‘Tyr1’Controlling Citrus Nematode Resistance

Based on the former constructed ＇Tyrl＇ locus genetic map in family 9145, from LB6-2 [Clementine mandarin （C. reticulata） × Hamlin orange （C. sinensis）] × Swingle citrumelo （C. paradise × P. trifoliata）, 9 markers were chosen for application in evaluating their effectiveness in marker-assisted selection （MAS） for citrus rootstock breeding program from many F1 progeny of Poncirus trifoliata. As the mapping revealed that these markers were estimated within a range of 12.1 cM in the linkage group, and among them, SCO07 co-segregated with ＂Tyrl＇, and 7A4R as the closest to ＇Tyrl＇ with a distance of 1.5 cM, these markers were basically fitful to go MAS screening. The results of screening P. trifoliata F1 progeny indicated that all the markers were inherited in codominant fashion and most of them were heterozygous on PT （Pomery of P. trifoliata）., marker 4L17R/CfoI and 7A4（1407）/BfaI were proved to be consistently reliable for accurate scoring of genotypes and the revealed polymorphism was basically coincided with the citrus nematode resistant phenotype within tested populations. The polymorphic genotype with marker 4L17R/Cfol was found completely matched up with the phenotype of individuals that conferred high resistance to citrus nematode when the USDA hybrid rootstocks were screened. Utilization of these markers, especially the highly specific 4L17R/Cfol and 7A4（1407）/Bfal, should result in great benefit to world citrus industry for early selection in rootstock-breeding program.

伴面神经和三叉神经受压的神经源性高血压的临床特征及显微血管减压术疗效评估

目的 探讨伴面神经、三叉神经受压的神经源性高血压的临床特征及采取显微血管减压术(MVD)对血压的影响。方法 回顾性分析2015年1月至2020年12月于福建医科大学附属第一医院神经外科治疗的伴面神经、三叉神经受压的神经源性高血压患者53例的资料。所有患者采用MVD进行治疗,记录术中、术后情况,观察术后3个月血压改变。根据血压下降幅度、药物使用情况及症状缓解程度进行疗效判定。治愈:完全停用降压药,收缩压<140和舒张压<90 mm Hg;显效:收缩压和舒张压中一项降至正常水平或收缩压下降值≥30 mm Hg和舒张压下降值≥10 mm Hg;部分缓解:收缩压下降值<30和舒张压下降值<10 mm Hg,高血压临床症状缓解,降压药用量减少;无效:高血压临床症状无缓解,收缩压和舒张压均较术前无明显变化甚至升高,且药物用量无变化甚至增加。有效包括治愈、显效及部分缓解。结果 面神经、三叉神经受压最常见的责任动脉为:小脑前下动脉(21例,占39.62%),小脑后下动脉(15例,占28.30%),小脑上动脉(11例,占20.75%)。与术前比较,术后3个月收缩压和舒张压下降[收缩压:(127.8±9.2)比(146.5±17.3)mm Hg,t=6.896,P<0.001;舒张压:(76.9±8.4)比(89.4±11.2)mm Hg,t=6.297,P<0.001]。治愈30例(56.60%),显效10例(18.87%),部分缓解5例(9.43%),无效8例(15.09%),显效率75.47%,有效率84.91%,且左侧神经受压患者术后有效率明显高于右侧神经受压患者[90.48%(19/21)比81.25%(26/32),χ^(2)=10.503,P=0.015]。所有患者的颅神经受压症状均消失并且未出现严重并发症。结论 MVD在治疗伴面神经、三叉神经受压的神经源性高血压方面有一定应用前景。

福建省老年人高血压患病情况调查及相关因素分析

目的了解福建省老年人高血压的患病情况及相关因素。方法使用2014年8月至2016年7月福建省高血压分层多阶段随机抽样横断面调查资料,抽取老年(年龄≥60岁)常驻居民4483人,观察高血压患病率以及知晓率、治疗率、控制率,比较不同性别之间、沿海及内陆之间、城市及乡村之间高血压患病情况的差别,分析高血压患病的相关因素。结果福建省老年居民的高血压患病率为60.2%,标化患病率59.1%,知晓率为55.8%,治疗率50.1%,控制率仅17.0%。其中女性高血压患病率(63.2%比57.1%)明显高于男性(P<0.05),知晓率(55.9%比55.6%),治疗率(50.6%比49.6%)及控制率(17.6%比16.6%)的差异无统计学意义(均P>0.05)。城乡间高血压患病率的差异无统计学意义(59.1%比61.4%,P>0.05),但城市高血压知晓率、治疗率及控制率明显高于乡村地区(60.2%比51.5%,56.1%比44.4%,25.4%比8.8%,均P<0.05)。与内陆地带相比,沿海地带患病率(77.0%比55.9%),及2、3级高血压患病率较高(25.9%比15.3%,15.5%比5.6%,均P<0.05)。非条件Logistic回归分析结果显示,福建省老年人群高血压的相关因素包括:年龄、中心性肥胖、文化程度、高血压家族史、身体脂肪率及体质量指数(BMI)。结论福建省老年人群高血压患病率较高,在女性、沿海地带及中心性肥胖人群中尤为明显,全省老年高血压患者的知晓率、治疗率及控制率仍偏低。