An 8-week feeding trial was conducted to determine the optimal dietary protein-to-lipid ratio for juvenile Nibea albiflora with an initial weight of(11.76 ± 0.20) g.Nine experimental diets containing different co...An 8-week feeding trial was conducted to determine the optimal dietary protein-to-lipid ratio for juvenile Nibea albiflora with an initial weight of(11.76 ± 0.20) g.Nine experimental diets containing different concentrations of protein(40%,47%,or 54%) and lipids(5%,9%,or 13%) in a 3 × 3 factorial experimental design were tested in triplicate groups of fish,while the protein-to-energy(P/E) ratios of the diets varied in the range of 19.74–28.32 mg k J^(-1).Results showed that fish fed diets containing 9% or 13% lipids with 54% protein exhibited significantly higher weight gains and specific growth rates than those fed other diets.The feed conversion rate of fish fed the diet with 40% protein and 5% lipids was significantly poorer than that of fish fed other diets.The protein efficiency rate of fish fed diets with 5% lipids was significantly lower than that of fish fed 9% or 13% lipid diets.Carcass lipid and energy contents were positively correlated with dietary lipid level regardless of protein level.Fish fed a 54% protein diet showed the highest trypsin activity.The intestinal lipase activity of fish fed the diet containing 13% lipids was significantly higher than that of fish fed 5% or 9% lipid diets.These results demonstrate the high protein dietary requirements of N.albiflora.A diet containing 54% protein and 9%–13% lipids with a P/E ratio of 26.2–27.81 mg protein k J^(-1) can be considered optimal for juvenile N.albiflora.展开更多
The impoundment of the Three Gorges Dam(TGD)has altered downstream hydrological characteristics and sediment discharge,and it has caused ecological impacts,such as changes in chlorophyll-a(Chl-a)in estuaries and coast...The impoundment of the Three Gorges Dam(TGD)has altered downstream hydrological characteristics and sediment discharge,and it has caused ecological impacts,such as changes in chlorophyll-a(Chl-a)in estuaries and coastal oceans.To investigate the TGD's influence on Chl-a's interannual and seasonal variations in the Changjiang Estuary and the adjacent coastal East China Sea,a physical-biogeochemical model was developed with numerical experiments covering a decade,including TGD's preperiod(pre-TGD,1999–2003)and post-period(post-TGD,2004–2008).The modeling results demonstrate an annual increase in the regional average Chl-a from pre-to post-TGD,with the largest increase reaching up to 20.8%in spring in the nearshore region beyond the Changjiang mouth.The spatial variations in Chl-a are high,with the largest variation being observed around the 20–40 m isobaths.The increase in Chl-a is influenced by improved light and nutrient conditions,which is attributed to dam construction and fertilization by human activities.A decline in nitrogen-phosphorus fertilizer usage ratio along the Changjiang River watershed after the TGD's impoundment is another factor that influences the Chl-a's variation.The modeling results also show severe NO3 and PO4 imbalances with a larger N/P ratio during the post-TGD period,especially in regions with large Chl-a increases.Moreover,the greater increase in the usage of phosphorus fertilizer than nitrogen fertilizer influences Chl-a's variation and has potential promotion effects on the outbreak of harmful algal bloom events.展开更多
基金supported by grants from the Na-tional Natural Science Foundation of China(No.41476127)the Science and Technology Planning Project of Zhejiang Province(No.2016F50038)+1 种基金the Science and Technology Planning Project of ZhouShan City(No.2015C31010)the Scientific Research Foundation of Zhejiang Ocean Universtiy(No.2014Q1434)
文摘An 8-week feeding trial was conducted to determine the optimal dietary protein-to-lipid ratio for juvenile Nibea albiflora with an initial weight of(11.76 ± 0.20) g.Nine experimental diets containing different concentrations of protein(40%,47%,or 54%) and lipids(5%,9%,or 13%) in a 3 × 3 factorial experimental design were tested in triplicate groups of fish,while the protein-to-energy(P/E) ratios of the diets varied in the range of 19.74–28.32 mg k J^(-1).Results showed that fish fed diets containing 9% or 13% lipids with 54% protein exhibited significantly higher weight gains and specific growth rates than those fed other diets.The feed conversion rate of fish fed the diet with 40% protein and 5% lipids was significantly poorer than that of fish fed other diets.The protein efficiency rate of fish fed diets with 5% lipids was significantly lower than that of fish fed 9% or 13% lipid diets.Carcass lipid and energy contents were positively correlated with dietary lipid level regardless of protein level.Fish fed a 54% protein diet showed the highest trypsin activity.The intestinal lipase activity of fish fed the diet containing 13% lipids was significantly higher than that of fish fed 5% or 9% lipid diets.These results demonstrate the high protein dietary requirements of N.albiflora.A diet containing 54% protein and 9%–13% lipids with a P/E ratio of 26.2–27.81 mg protein k J^(-1) can be considered optimal for juvenile N.albiflora.
基金supported by grants from the GuangdongNSFC Joint Theme Project(No.U1701247)the National Natural Science Foundation of China(No.91328203)+1 种基金the Southern Marine Science and Engineering–Guangdong Laboratory(Zhuhai)(No.311019006)the Sun Yat-sen University Supercomputing Funding(No.42000-52603700)。
文摘The impoundment of the Three Gorges Dam(TGD)has altered downstream hydrological characteristics and sediment discharge,and it has caused ecological impacts,such as changes in chlorophyll-a(Chl-a)in estuaries and coastal oceans.To investigate the TGD's influence on Chl-a's interannual and seasonal variations in the Changjiang Estuary and the adjacent coastal East China Sea,a physical-biogeochemical model was developed with numerical experiments covering a decade,including TGD's preperiod(pre-TGD,1999–2003)and post-period(post-TGD,2004–2008).The modeling results demonstrate an annual increase in the regional average Chl-a from pre-to post-TGD,with the largest increase reaching up to 20.8%in spring in the nearshore region beyond the Changjiang mouth.The spatial variations in Chl-a are high,with the largest variation being observed around the 20–40 m isobaths.The increase in Chl-a is influenced by improved light and nutrient conditions,which is attributed to dam construction and fertilization by human activities.A decline in nitrogen-phosphorus fertilizer usage ratio along the Changjiang River watershed after the TGD's impoundment is another factor that influences the Chl-a's variation.The modeling results also show severe NO3 and PO4 imbalances with a larger N/P ratio during the post-TGD period,especially in regions with large Chl-a increases.Moreover,the greater increase in the usage of phosphorus fertilizer than nitrogen fertilizer influences Chl-a's variation and has potential promotion effects on the outbreak of harmful algal bloom events.