2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN or TTF), which possesses aggregation-induced emission (AIE) characteristic, is doped in organically modified silica (ORMOSIL) na...2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN or TTF), which possesses aggregation-induced emission (AIE) characteristic, is doped in organically modified silica (ORMOSIL) nanoparticles. By increasing the weight ratio of TTF to the precursor of silica nanoparticles (the quantities of the precursors were kept the same), the fluorescence intensity of nanoparticles increased correspondingly, due to the formation of larger AIE dots in the cores of ORMOSIL nanoparticles. The fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa cells. Two-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability, as well as cytoplasm staining towards tumor cells. Our experimental results may offer a promising method for fast and bright fluorescence imaging, as well as bio-molecule/drug delivery to cell nucleus.展开更多
2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl)fumaronitrile(TPE-TPA-FN or TTF),which possesses aggregation-induced emission(AIE)characteristic,is doped in organically modified silica(ORMOSIL)nanopartic...2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl)fumaronitrile(TPE-TPA-FN or TTF),which possesses aggregation-induced emission(AIE)characteristic,is doped in organically modified silica(ORMOSIL)nanoparticles.By increasing the weight ratio of TTF to the precursor of silica nanoparticles(the quantities of the precursors were kept the same),the fluorescence intensity of nanoparticles increased correspondingly,due to the formation of larger AIE dots in the cores of ORMOSIL nanoparticles.The fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa cells.Two-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability,as well as cytoplasm staining towards tumor cells.Our experimental results may offer a promising method for fast and bright fluorescence imaging,as well as bio-molecule/drug delivery to cell nucleus.展开更多
文摘为了提高丙烯酸树脂的耐水性、附着力以及耐溶剂性,以桐油酸和环氧树脂E-44为原料制备环氧酯,采用溶液聚合和自乳化工艺合成了环氧酯改性水性丙烯酸树脂,并引入氰特CY325氨基树脂制备双组分环氧酯改性水性丙烯酸树脂漆膜。利用FT-IR、1 H NMR、粒径测试等对环氧酯单体、环氧酯改性水性丙烯酸树脂的结构和性能进行表征和分析,并测试了单组分和双组分环氧酯改性水性丙烯酸树脂漆膜的硬度、光泽、吸水率、水接触角、耐溶剂性等性能。结果表明:当环氧酯用量为35%时,单/双组分漆膜综合性能达到最佳,双组分漆膜光泽(60°)达102.3,耐溶剂擦拭次数为500次,耐水性可达480 h,附着力为0级,铅笔硬度为4H,耐冲击性为50 cm。
基金the National Basic Research Program of China (973 Program, 2013CB834704 and 2011CB503700)the National Natural Science Foundation of China (61275190)
文摘2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl) fumaronitrile (TPE-TPA-FN or TTF), which possesses aggregation-induced emission (AIE) characteristic, is doped in organically modified silica (ORMOSIL) nanoparticles. By increasing the weight ratio of TTF to the precursor of silica nanoparticles (the quantities of the precursors were kept the same), the fluorescence intensity of nanoparticles increased correspondingly, due to the formation of larger AIE dots in the cores of ORMOSIL nanoparticles. The fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa cells. Two-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability, as well as cytoplasm staining towards tumor cells. Our experimental results may offer a promising method for fast and bright fluorescence imaging, as well as bio-molecule/drug delivery to cell nucleus.
文摘2,3-Bis(4-(phenyl(4-(1,2,2-triphenylvinyl)phenyl)amino)phenyl)fumaronitrile(TPE-TPA-FN or TTF),which possesses aggregation-induced emission(AIE)characteristic,is doped in organically modified silica(ORMOSIL)nanoparticles.By increasing the weight ratio of TTF to the precursor of silica nanoparticles(the quantities of the precursors were kept the same),the fluorescence intensity of nanoparticles increased correspondingly,due to the formation of larger AIE dots in the cores of ORMOSIL nanoparticles.The fluorescent and biocompatible nanoprobes were then utilized for in vitro imaging of HeLa cells.Two-photon fluorescence microscopy clearly illustrated that the nanoparticles have the capacity of nucleus permeability,as well as cytoplasm staining towards tumor cells.Our experimental results may offer a promising method for fast and bright fluorescence imaging,as well as bio-molecule/drug delivery to cell nucleus.
