以江苏省上表矿区、探矿权、采矿权等数据为研究案例,基于WebGIS技术架构,采用Java作为开发语言,数据库使用MySQL,前端采用ArcGIS API for JavaScript,开发了一套建设项目压覆矿产资源查询服务系统,实现建设项目压覆矿产资源的在线分析...以江苏省上表矿区、探矿权、采矿权等数据为研究案例,基于WebGIS技术架构,采用Java作为开发语言,数据库使用MySQL,前端采用ArcGIS API for JavaScript,开发了一套建设项目压覆矿产资源查询服务系统,实现建设项目压覆矿产资源的在线分析查询功能。系统具有简单易用、普适性强、扩展性好等特点,可为建设单位进行远程压矿预查提供极大的便利,从而有效提高相关部门行政审批的工作效率。展开更多
The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.Howev...The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.However,the development of rice trichomes remains poorly understood.In this study,we conducted ethylmethane sulfonate(EMS)-mediated mutagenesis on the wild-type(WT)indica rice‘Xida 1B’.Phenotypic analysis led to the screening of a mutant that is defective in trichome development,designated lhl1(less hairy leaf 1).We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome 2.The locus LOC_Os02g25230 was identified as the candidate gene by sequencing.We constructed RNA interference(LHL1-RNAi)and overexpression lines(LHL1-OE)to verity the candidate gene.The leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant,whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT,although the number of trichomes was significantly higher.Quantitative real-time PCR(RT-qPCR)analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the WT.Hormone response analysis revealed that LHL1 expression was affected by auxin.The results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.展开更多
文摘以江苏省上表矿区、探矿权、采矿权等数据为研究案例,基于WebGIS技术架构,采用Java作为开发语言,数据库使用MySQL,前端采用ArcGIS API for JavaScript,开发了一套建设项目压覆矿产资源查询服务系统,实现建设项目压覆矿产资源的在线分析查询功能。系统具有简单易用、普适性强、扩展性好等特点,可为建设单位进行远程压矿预查提供极大的便利,从而有效提高相关部门行政审批的工作效率。
基金supported by grants from the Natural Science Foundation of Chongqing,China(cstc2020jcyj-msxm0539,cstc2015jcyjA80008)the National College Students Innovation and Entrepreneurship Training Program from the Ministry of Education,China(202110635082)the National Natural Science Foundation of China(32171964,31171178).
文摘The trichomes of rice leaves are formed by the differentiation and development of epidermal cells.Plant trichomes play an important role in stress resistance and protection against direct ultraviolet irradiation.However,the development of rice trichomes remains poorly understood.In this study,we conducted ethylmethane sulfonate(EMS)-mediated mutagenesis on the wild-type(WT)indica rice‘Xida 1B’.Phenotypic analysis led to the screening of a mutant that is defective in trichome development,designated lhl1(less hairy leaf 1).We performed map-based cloning and localized the mutated gene to the 70-kb interval between the molecular markers V-9 and V-10 on chromosome 2.The locus LOC_Os02g25230 was identified as the candidate gene by sequencing.We constructed RNA interference(LHL1-RNAi)and overexpression lines(LHL1-OE)to verity the candidate gene.The leaves of the LHL1-RNAi lines showed the same trichome developmental defects as the lhl1 mutant,whereas the trichome morphology on the leaf surface of the LHL1-OE lines was similar to that of the WT,although the number of trichomes was significantly higher.Quantitative real-time PCR(RT-qPCR)analysis revealed that the expression levels of auxin-related genes and positive regulators of trichome development in the lhl1 mutant were down-regulated compared with the WT.Hormone response analysis revealed that LHL1 expression was affected by auxin.The results indicate that the influence of LHL1 on trichome development in rice leaves may be associated with an auxin pathway.
