The expressed proteins were extracted from human benign prostatic hyperplastic tissues obtained with transurethral resection of the prostate before and after their irradiation with radioactive nuclide. The proteins we...The expressed proteins were extracted from human benign prostatic hyperplastic tissues obtained with transurethral resection of the prostate before and after their irradiation with radioactive nuclide. The proteins were separated by two-dimensional gel electrophoresis and analyzed by mass spectrometry. Four proteins were differentially expressed and were identified with a database search. Three were associated with the regulation of cell motion and one was lactate dehydrogenase B, which plays an important role in the process of cell energy metabolism and cell state changes in tissue. The comparative proteomic analysis indicates that after irradiation with radioactive nuclide, changes occur in human benign prostatic hyperplastic cells, and associated proteins are expressed. Analysis of these proteins should help to identify the mechanism involved when human prostatic hyperplasia is treated by irradiation with radioactive nuclide.展开更多
目的建立微波消解-电感耦合等离子体质谱测定法(microwave digestion-inductively coupledplasma mass spectrometry,MD-ICP-MS)同时检测保健食品中钙、铁、锌营养元素的方法。方法样品经过硝酸-过氧化氢微波消解预处理后,电感耦合等离...目的建立微波消解-电感耦合等离子体质谱测定法(microwave digestion-inductively coupledplasma mass spectrometry,MD-ICP-MS)同时检测保健食品中钙、铁、锌营养元素的方法。方法样品经过硝酸-过氧化氢微波消解预处理后,电感耦合等离子体质谱法测定钙铁锌口服液中钙、铁、锌的含量。以45Sc和72Ge为内标溶液在线加入来校正。结果该方法的线性关系良好,相关系数(r)为0.9997~0.9998;方法的检出限为钙3.8μg/mL,铁0.013μg/mL,锌0.0792μg/mL;精密度RSD<2%,重复性RSD<2%,加标回收率为93.4%~104.3%。结论该方法具有线性范围宽、精密度高、操作简便、快速检测等优点,可作为检测钙铁锌口服液中钙、铁、锌元素的有效快速检测手段。展开更多
Accurately calculating detachment capacity is the most fundamental issue when establishing a soil erosion process model.Colluvial deposits of Benggang are typical soil-gravel mixtures,whereas the understanding of the ...Accurately calculating detachment capacity is the most fundamental issue when establishing a soil erosion process model.Colluvial deposits of Benggang are typical soil-gravel mixtures,whereas the understanding of the soil detachment of colluvial deposits is limited.This work investigated the effects of the gravel contents on the soil detachment capacity of colluvial deposits and its hydrodynamic mechanism.The colluvial sample was collected in Anxi County,Fujian Province,Southeast China,and a small-sample scouring test was used.The slope steepness ranged from 18%to 84%,unit discharge ranged from 0.56×10^(-3)to 2.22×10^(-3)m^(2)s^(-1),and gravel content ranged from 0%to 70%.The results indicated that the gravel content is the primary factor that influences the detachment capacity,followed by the discharge and then the slope.The detachment capacity trend with the gravel content varied over different slopes and discharges.Stream power represents the best hydrodynamic parameter for modelling the detachment capacity of colluvial deposits and can be used to establish a fitting equation for the colluvium together with the mean weight diameter(MWD)(Nash-Sutcliffe efficiency(NSE)=0.96).As the gravel content increased,the soil erodibility parameters increased several folds,in some cases more than 10 folds,mainly because the soil shear strength decreased gradually.Meanwhile,as the gravel content increased,the gravel specific surface area increased,the obstruction of gravel to runoff increased,and the energy needed for runoff to overcome gravel obstruction increased,leading to 2-3 folds higher critical shear stress of runoff for soilgravel mixtures compared with pure soil.In summary,gravel can influence the detachment capacity by changing the soil properties,and the gravel content also affects the relationship between soil detachment capacity and the hydrodynamic parameters.These findings deepen the understanding of the influence of gravel on soil erosion and provide a basis for establishing a soil erosion process model in colluvial deposits.展开更多
基金Supported by the WU Jie-ping Medical Foundation of China(No.32027400501)
文摘The expressed proteins were extracted from human benign prostatic hyperplastic tissues obtained with transurethral resection of the prostate before and after their irradiation with radioactive nuclide. The proteins were separated by two-dimensional gel electrophoresis and analyzed by mass spectrometry. Four proteins were differentially expressed and were identified with a database search. Three were associated with the regulation of cell motion and one was lactate dehydrogenase B, which plays an important role in the process of cell energy metabolism and cell state changes in tissue. The comparative proteomic analysis indicates that after irradiation with radioactive nuclide, changes occur in human benign prostatic hyperplastic cells, and associated proteins are expressed. Analysis of these proteins should help to identify the mechanism involved when human prostatic hyperplasia is treated by irradiation with radioactive nuclide.
文摘目的建立微波消解-电感耦合等离子体质谱测定法(microwave digestion-inductively coupledplasma mass spectrometry,MD-ICP-MS)同时检测保健食品中钙、铁、锌营养元素的方法。方法样品经过硝酸-过氧化氢微波消解预处理后,电感耦合等离子体质谱法测定钙铁锌口服液中钙、铁、锌的含量。以45Sc和72Ge为内标溶液在线加入来校正。结果该方法的线性关系良好,相关系数(r)为0.9997~0.9998;方法的检出限为钙3.8μg/mL,铁0.013μg/mL,锌0.0792μg/mL;精密度RSD<2%,重复性RSD<2%,加标回收率为93.4%~104.3%。结论该方法具有线性范围宽、精密度高、操作简便、快速检测等优点,可作为检测钙铁锌口服液中钙、铁、锌元素的有效快速检测手段。
基金funded primarily by grants from the Natural Science Foundation of Fujian Province of China(2021J01120)the National Natural Science Foundation of China(41977071)。
文摘Accurately calculating detachment capacity is the most fundamental issue when establishing a soil erosion process model.Colluvial deposits of Benggang are typical soil-gravel mixtures,whereas the understanding of the soil detachment of colluvial deposits is limited.This work investigated the effects of the gravel contents on the soil detachment capacity of colluvial deposits and its hydrodynamic mechanism.The colluvial sample was collected in Anxi County,Fujian Province,Southeast China,and a small-sample scouring test was used.The slope steepness ranged from 18%to 84%,unit discharge ranged from 0.56×10^(-3)to 2.22×10^(-3)m^(2)s^(-1),and gravel content ranged from 0%to 70%.The results indicated that the gravel content is the primary factor that influences the detachment capacity,followed by the discharge and then the slope.The detachment capacity trend with the gravel content varied over different slopes and discharges.Stream power represents the best hydrodynamic parameter for modelling the detachment capacity of colluvial deposits and can be used to establish a fitting equation for the colluvium together with the mean weight diameter(MWD)(Nash-Sutcliffe efficiency(NSE)=0.96).As the gravel content increased,the soil erodibility parameters increased several folds,in some cases more than 10 folds,mainly because the soil shear strength decreased gradually.Meanwhile,as the gravel content increased,the gravel specific surface area increased,the obstruction of gravel to runoff increased,and the energy needed for runoff to overcome gravel obstruction increased,leading to 2-3 folds higher critical shear stress of runoff for soilgravel mixtures compared with pure soil.In summary,gravel can influence the detachment capacity by changing the soil properties,and the gravel content also affects the relationship between soil detachment capacity and the hydrodynamic parameters.These findings deepen the understanding of the influence of gravel on soil erosion and provide a basis for establishing a soil erosion process model in colluvial deposits.
