Human epididymal protein 4(HE4),carbohydrate antigen 125(CA125)and osteopontin(OPN)are three key biomarkers in detecting ovarian cancer.To explore the diagnosis value of combined detection of these three biomarkers fo...Human epididymal protein 4(HE4),carbohydrate antigen 125(CA125)and osteopontin(OPN)are three key biomarkers in detecting ovarian cancer.To explore the diagnosis value of combined detection of these three biomarkers for ovarian cancer,we developed a multiplexed assay on a plasmonic gold(pGOLD)platform for measuring HE4,CA125 and OPN in urine.The receiver operator characteristic(ROC)curve was drawn,and the diagnosis values of each biomarker alone or in combination for ovarian cancer were evaluated.In the analysis to distinguish ovarian cancer from other gynecological cancers,ovarian cysts and healthy people,the sensitivities of HE4,CA125 and OPN were 72.55%,52.82%and 68.63%,the specificity values were 95.06%,87.65%and 90.12%,while the areas under the curve(AUC)were 0.85,0.75 and 0.77,respectively.The sensitivity and specificity for combination detection of the three markers were 90.20%and 80.25%.The detection methods of HE4,CA125 and OPN based on plasma fluorescence enhanced chip showed good analytic and diagnostic performance,and provided a non-invasive method for the diagnosis of ovarian cancer.展开更多
基金supported by the Research and Development Projects in Key Areas of Guangdong Province,China(No.2020B1111160003)the Science and Technology Planning Project of Shenzhen City,China(No.JSGG20191231141403880)+3 种基金the National Natural Science Foundation of China(No.81801749)the Key Research and Development Program of Hunan Province,China(No.2022SK2053)the Science and Technology Innovation Program of Hunan Province“Huxiang Young Talents Plan”,China(No.2021RC3106)the Science and Technology Foundation of Shenzhen City,China(No.JCYJ20190812164009243).
文摘Human epididymal protein 4(HE4),carbohydrate antigen 125(CA125)and osteopontin(OPN)are three key biomarkers in detecting ovarian cancer.To explore the diagnosis value of combined detection of these three biomarkers for ovarian cancer,we developed a multiplexed assay on a plasmonic gold(pGOLD)platform for measuring HE4,CA125 and OPN in urine.The receiver operator characteristic(ROC)curve was drawn,and the diagnosis values of each biomarker alone or in combination for ovarian cancer were evaluated.In the analysis to distinguish ovarian cancer from other gynecological cancers,ovarian cysts and healthy people,the sensitivities of HE4,CA125 and OPN were 72.55%,52.82%and 68.63%,the specificity values were 95.06%,87.65%and 90.12%,while the areas under the curve(AUC)were 0.85,0.75 and 0.77,respectively.The sensitivity and specificity for combination detection of the three markers were 90.20%and 80.25%.The detection methods of HE4,CA125 and OPN based on plasma fluorescence enhanced chip showed good analytic and diagnostic performance,and provided a non-invasive method for the diagnosis of ovarian cancer.
