响应面法优化霍山石斛三萜皂苷提取工艺研究

为了探讨三萜皂苷在霍山石斛不同部位分布差异,以霍山石斛全株为原材料,以三萜皂苷的提取得率为考查指标,在单因素实验基础上,采用响应面法探讨霍山石斛的三萜皂苷的最佳提取工艺。采用最佳提取方法,对霍山石斛根、茎、叶中三萜皂苷的含量进行对比研究。结果表明,霍山石斛的三萜皂苷最佳提取工艺为:甲醇浓度35%、液料比30∶1(mL/g)、提取时间1.5 h、提取温度64℃、提取次数4次,在此条件下,测得霍山石斛根、茎、叶中三萜皂苷含量分别为3.46%、0.85%、2.47%。研究结果可望为霍山石斛资源综合开发利用提供参考依据。

六安市中药产业发展现状与存在问题研究

为了更好地发展和规划六安市中药产业,我们对其生产现状和存在问题进行了实地调查。调查发现,六安市中药农业发展较好,中药工业薄弱,中药大健康产业少。建议从做强中药农业,做大中药工业,做精中药大健康产业三个方面进行规划,使六安市中药产业形成科学的发展模式。

河南石斛游离氨基酸超声辅助提取工艺条件的优化

为河南石斛药效物质基础研究及资源开发利用奠定基础,以河南石斛游离氨基酸提取得率为考察指标,在单因素试验基础上,利用响应面法对河南石斛游离氨基酸超声辅助提取工艺的参数进行优化。结果表明:河南石斛游离氨基酸超声辅助提取的最佳工艺条件为料液比1︰40(mL∶g)、超声提取时间30min、超声功率240 W、提取4次。在此条件下,河南石斛的游离氨基酸含量为(1.95±0.12)%,与理论值误差较小,通过Design-Expert软件建立的回归模型准确可行,优化工艺稳定可靠。

基于星点设计-效应面法优化黄精咀嚼片制备工艺

制备黄精咀嚼片并优化其处方工艺。以黄精为原料,甘露醇、预胶化淀粉、羟丙基纤维素、木糖醇、山梨醇等为辅料;采用湿法制粒压片,以咀嚼片外观、口感、风味和硬度为指标,在单因素试验基础上,以星点设计效应面法进一步优化处方。结果表明最优处方为:预胶化淀粉与甘露醇的比例为4.21∶1,用量为69.38%,山梨醇木糖醇比例为1∶1,用量7.49%,黏合剂为羟丙纤维素的水溶液,滑石粉适量。通过处方筛选和工艺优化可制得口感适宜、表面光滑美观、色泽均匀、硬度适中的咀嚼片。为黄精深加工产品开发提供理论参考依据。

Effects of ecological factors on secondary metabolites and inorganic elements of Scutellaria baicalensis and analysis of geoherblism

This study analyzed the effects of ecological factors on secondary metabolites of Scutellaria baicalensis using two sources:92individual roots of S.baicalensis from all over China,and secondary metabolites,medicinal materials and inorganic element contents obtained from the testing of 92 S.baicalensis rhizosphere soil samples.The study used environmental data from the Genuine Medicinal Material Spatial Analysis Database.Most of the chemical constituents of S.baicalensis were negatively correlated to latitude and positively correlated to temperature;generally,the contents of 21 chemical constituents were higher at low latitudes than that at high latitudes.By gradual regression analysis,it was found that the content of baicalin in S.baicalensis was negatively correlated to latitude and generally the content of inorganic elements in soil was excessively high(excluding Mg and Ca),which has a negative effect on the accumulation of chemical constituents in S.baicalensis.Based on the cluster analysis of 21 constituents,S.baicalensis from different places of origin was divided into two groups,and S.baicalensis was not genuine only in a specific small region.Within the zone from Chifeng,Inner Mongolia to Taibai,Shaanxi is suitable for accumulation of secondary metabolites of S.baicalensis and such a zone represents a suitable distribution and potential genuine producing area.