Background The SLAM family recently has been reported to show an important biological role in lymphocyte development and immunological function, and it is efficient to highly purify hematopoietic stem cells using a si...Background The SLAM family recently has been reported to show an important biological role in lymphocyte development and immunological function, and it is efficient to highly purify hematopoietic stem cells using a simple combination of SLAM family members. To elucidate the presence of this family on acute lymphoblastic leukemia （ALL）,as well as its relationship with the leukemia-initiating potential, we analyzed the expression pattern of this family members on human ALL progenitor cells, combined with serial xenotransplantation assay.Methods Expression analysis was carried out by flow cytometry. We combined the expression pattern of human CD150,CD244 and CD48 with serial xenotransplantation of B-ALL progenitor cells to indicate their relationship.Results CD48 and CD244 were expressed on most B-ALL progenitor cells, the percentage being （93.08±6.46）% and （63.37±29.31）%, respectively. Interestingly, the proportion of CD150＋ cells declined obviously in engrafted cases （（24.94±7.32）%） compared with non-engrafted cases （（77.54±5.93）%, P 〈0.01）, which indicated that only blast cells with low percentage of CD150＋ population were able to reconstitute leukemia into primary, secondary and tertiary NOD/SCID mice.Conclusions SLAM family members are present on B-ALL progenitor cells and the leukemia-initiating potential of leukemic blasts is correlated negatively with the proportion of CD150＋ cells, the percentage of which can serve as a useful predictor for engraftment success of B-ALL to immune deficient mice.展开更多
文摘Background The SLAM family recently has been reported to show an important biological role in lymphocyte development and immunological function, and it is efficient to highly purify hematopoietic stem cells using a simple combination of SLAM family members. To elucidate the presence of this family on acute lymphoblastic leukemia （ALL）,as well as its relationship with the leukemia-initiating potential, we analyzed the expression pattern of this family members on human ALL progenitor cells, combined with serial xenotransplantation assay.Methods Expression analysis was carried out by flow cytometry. We combined the expression pattern of human CD150,CD244 and CD48 with serial xenotransplantation of B-ALL progenitor cells to indicate their relationship.Results CD48 and CD244 were expressed on most B-ALL progenitor cells, the percentage being （93.08±6.46）% and （63.37±29.31）%, respectively. Interestingly, the proportion of CD150＋ cells declined obviously in engrafted cases （（24.94±7.32）%） compared with non-engrafted cases （（77.54±5.93）%, P 〈0.01）, which indicated that only blast cells with low percentage of CD150＋ population were able to reconstitute leukemia into primary, secondary and tertiary NOD/SCID mice.Conclusions SLAM family members are present on B-ALL progenitor cells and the leukemia-initiating potential of leukemic blasts is correlated negatively with the proportion of CD150＋ cells, the percentage of which can serve as a useful predictor for engraftment success of B-ALL to immune deficient mice.
