目的比较3种麻醉方式在老年髋部骨折手术治疗中的应用效果。方法将60例老年髋部骨折手术患者随机分为3组,每组20例:N-B组采取腰丛+坐骨神经阻滞麻醉,G-A组采取气管插管全身麻醉,G-N组采取气管插管全身麻醉联合腰丛+坐骨神经阻滞麻醉。比...目的比较3种麻醉方式在老年髋部骨折手术治疗中的应用效果。方法将60例老年髋部骨折手术患者随机分为3组,每组20例:N-B组采取腰丛+坐骨神经阻滞麻醉,G-A组采取气管插管全身麻醉,G-N组采取气管插管全身麻醉联合腰丛+坐骨神经阻滞麻醉。比较:①3组手术时间、术中出血量;②3组血流动力学指标;③3组术后不良反应发生例数;④G-A组和G-N组的全身麻醉用药总量、拔管时间及拔管后躁动率;⑤3组不同时段的VAS评分。结果①3组手术时间、术中出血量比较差异无统计学意义(P>0.05)。②T4时,N-B组动脉压、心率均高于G-A组和G-N组(P<0.05),G-A组动脉压、心率均高于G-N组(P<0.05);T5时,N-B组动脉压、心率仍均高于G-A组和G-N组(P<0.05)。③G-N组、N-B组术后不良反应总发生例数均低于G-A组(P<0.05)。④全身麻醉用药总量和拔管时间G-N组均低于G-A组(P<0.05)。⑤G-N组术后12、24 h VAS评分均低于N-B组和G-A组(P<0.05)。结论老年髋部骨折患者手术采用气管插管全身麻醉联合腰丛+坐骨神经阻滞麻醉,术中血流动力学稳定,术后不良反应发生率低,可提供比较确切的术后镇痛,麻醉效果安全可靠。展开更多
To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae,a cDNA library for suppression subtractive hybridization （SSH） was constructed with cDNAs from soybean cultiv...To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae,a cDNA library for suppression subtractive hybridization （SSH） was constructed with cDNAs from soybean cultivar Suinong 10 treated with sterile distilled water as the driver and cDNAs from Suinong 10 inoculated with P.sojae as the tester.A total of 2 067 recombinant colonies from the SSH library were randomly picked,amplified,and sequenced.After discarding 312 poor quality expressed sequence tags （EST）,1 755 high quality ESTs were assembled and edited to 1 384 tentatively unique genes （TUG）,in which,586 showed significant homology to known sequences,and 798 had low homology or no match with the known sequences.A cDNA microarray containing 307 singletons from the 586 TUGs and 222 singletons from the 798 TUGs was developed to characterize differentially expressed cDNAs in the SSH library,and eight cDNAs were identified to be up-regulated after microarray analysis and then confirmed by real-time PCR.They were homologous to the protein 10,and were also related to some proteins in disease resistance response,such as pathogen-related protein,phenylalanine ammonia-lyase,isoflavone reductase,WRKY transcription factor 31,major allergen Pru ar 1,and pleiotropic drug resistance protein 12.Most of the up-regulated cDNAs encode enzymes of phytoalexin biosynthesis and pathogenesis-related proteins involved in plant disease resistance.Here,we fist reported the Pru ar 1 in soybeans.The findings of this research have contributed to better understanding of soybean resistance to P.sojae at the molecular level.展开更多
文摘目的比较3种麻醉方式在老年髋部骨折手术治疗中的应用效果。方法将60例老年髋部骨折手术患者随机分为3组,每组20例:N-B组采取腰丛+坐骨神经阻滞麻醉,G-A组采取气管插管全身麻醉,G-N组采取气管插管全身麻醉联合腰丛+坐骨神经阻滞麻醉。比较:①3组手术时间、术中出血量;②3组血流动力学指标;③3组术后不良反应发生例数;④G-A组和G-N组的全身麻醉用药总量、拔管时间及拔管后躁动率;⑤3组不同时段的VAS评分。结果①3组手术时间、术中出血量比较差异无统计学意义(P>0.05)。②T4时,N-B组动脉压、心率均高于G-A组和G-N组(P<0.05),G-A组动脉压、心率均高于G-N组(P<0.05);T5时,N-B组动脉压、心率仍均高于G-A组和G-N组(P<0.05)。③G-N组、N-B组术后不良反应总发生例数均低于G-A组(P<0.05)。④全身麻醉用药总量和拔管时间G-N组均低于G-A组(P<0.05)。⑤G-N组术后12、24 h VAS评分均低于N-B组和G-A组(P<0.05)。结论老年髋部骨折患者手术采用气管插管全身麻醉联合腰丛+坐骨神经阻滞麻醉,术中血流动力学稳定,术后不良反应发生率低,可提供比较确切的术后镇痛,麻醉效果安全可靠。
基金supported by the Program for New Century Excellent Talents in Universities,Ministry of Education,China(NCET-09-164)the National Natural Science Foundation of China(30671317,30971811,31071439,and 31110103001)+1 种基金the Program for New Century Excellent Talents in Universities in Heilongjiang Province,China(NCET-06-007)the Natural Science Foundation of Heilongjiang Province,China(C200814)
文摘To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae,a cDNA library for suppression subtractive hybridization （SSH） was constructed with cDNAs from soybean cultivar Suinong 10 treated with sterile distilled water as the driver and cDNAs from Suinong 10 inoculated with P.sojae as the tester.A total of 2 067 recombinant colonies from the SSH library were randomly picked,amplified,and sequenced.After discarding 312 poor quality expressed sequence tags （EST）,1 755 high quality ESTs were assembled and edited to 1 384 tentatively unique genes （TUG）,in which,586 showed significant homology to known sequences,and 798 had low homology or no match with the known sequences.A cDNA microarray containing 307 singletons from the 586 TUGs and 222 singletons from the 798 TUGs was developed to characterize differentially expressed cDNAs in the SSH library,and eight cDNAs were identified to be up-regulated after microarray analysis and then confirmed by real-time PCR.They were homologous to the protein 10,and were also related to some proteins in disease resistance response,such as pathogen-related protein,phenylalanine ammonia-lyase,isoflavone reductase,WRKY transcription factor 31,major allergen Pru ar 1,and pleiotropic drug resistance protein 12.Most of the up-regulated cDNAs encode enzymes of phytoalexin biosynthesis and pathogenesis-related proteins involved in plant disease resistance.Here,we fist reported the Pru ar 1 in soybeans.The findings of this research have contributed to better understanding of soybean resistance to P.sojae at the molecular level.
