Plant calcineurin B-like （CBL） proteins have been proposed as important Ca2＋ sensors and specifically interact with CBL-interacting protein kinases （CIPKs） in plant-specific calcium signaling. Here, we identified...Plant calcineurin B-like （CBL） proteins have been proposed as important Ca2＋ sensors and specifically interact with CBL-interacting protein kinases （CIPKs） in plant-specific calcium signaling. Here, we identified and isolated 15 CIPK genes in a japonica rice variety Nipponbare based on the predicted sequences of rice CIPK gene family. Gene structure analysis showed that these 15 genes were divided into intron-less and intron-rich groups, and OsCIPK3 and OsCIPK24 exhibited alternative splicing in their mature process. The phylogenetic analyses indicated that rice CIPKs shared an ancestor with Arabidopsis and poplar CIPKs. Analyses of gene expression showed that these OsCIPK genes were differentially induced by biotic stresses such as bacterial blight and abiotic stresses （heavy metal such as Hg2＋, high salinity, cold and ABA）. Interestingly, five OsCIPK genes, OsCIPK1, 2, 10, 11 and 12, were transcriptionally up-regulated after bacterial blight infection whereas four OsCIPK genes, OsCIPK2, 10, 11 and 14, were induced by all treatments, indicating that some of OsCIPK genes are involved in multiple stress response pathways in plants. Our finding suggests that CIPKs play a key role in both biotic and abiotic stress responses.展开更多
The homozygous T3 transgenic lines with sense OsCBL8 gene and antisense OsCBL8 gene obtained by agro-transformation were used to investigate the function of OsCBL8 in rice. Semi-quantitative RT-PCR showed that the exp...The homozygous T3 transgenic lines with sense OsCBL8 gene and antisense OsCBL8 gene obtained by agro-transformation were used to investigate the function of OsCBL8 in rice. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic lines, and decreased to some extents in antisense transgenic lines. Such up- and down-regulation of the OsCBL8 gene in these transgenic lines had little effects on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some of transgenic lines. By evaluation of the tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, and relevant physiological indices, 8F12, a sense transgenic line with high salt tolerance, and 8R14, an antisense transgenic line with high drought tolerance, were obtained, which suggests that the OsCBL8 gene is involved in the response of rice to abiotic stresses.展开更多
The vacuolar proton pump ATPase（V-H^＋-ATPase）, which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several ...The vacuolar proton pump ATPase（V-H^＋-ATPase）, which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H^＋-ATPase subunits B（Sc VHA-B, Gen Bank： JF826506）, C（Sc VHA-C, Gen Bank： JF826507） and H（Sc VHA-H, Gen Bank： JF826508） were isolated from the halophyte Suaeda corniculata. The transcript levels of Sc VHA-B, Sc VHA-C and Sc VHA-H were increased by salt, drought and saline-alkali treatments. V-H^＋-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H^＋-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H^＋-ATPase subunits B, C and H（Sc VHA-B, Sc VHA-C and Sc VHA-H） from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type（WT）. More free proline, higher superoxide dismutase（SOD） activity and lower malondialdehyde（MDA） levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the Sc VHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of Sc VHA-B, Sc VHA-C and Sc VHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.展开更多
基金supported by the National Natural Science Foundation of China (Grants Nos.30800677 and 30771329)the Natural Science Foundation of Zhejiang Province, China (Grant No. Y3080359)+1 种基金National Key Programs for Transgenic Crops (Grant Nos. 2008ZX08009-001 and 2009ZX08009-076B)Zhejiang Normal University Innovative Research Team Program, China
文摘Plant calcineurin B-like （CBL） proteins have been proposed as important Ca2＋ sensors and specifically interact with CBL-interacting protein kinases （CIPKs） in plant-specific calcium signaling. Here, we identified and isolated 15 CIPK genes in a japonica rice variety Nipponbare based on the predicted sequences of rice CIPK gene family. Gene structure analysis showed that these 15 genes were divided into intron-less and intron-rich groups, and OsCIPK3 and OsCIPK24 exhibited alternative splicing in their mature process. The phylogenetic analyses indicated that rice CIPKs shared an ancestor with Arabidopsis and poplar CIPKs. Analyses of gene expression showed that these OsCIPK genes were differentially induced by biotic stresses such as bacterial blight and abiotic stresses （heavy metal such as Hg2＋, high salinity, cold and ABA）. Interestingly, five OsCIPK genes, OsCIPK1, 2, 10, 11 and 12, were transcriptionally up-regulated after bacterial blight infection whereas four OsCIPK genes, OsCIPK2, 10, 11 and 14, were induced by all treatments, indicating that some of OsCIPK genes are involved in multiple stress response pathways in plants. Our finding suggests that CIPKs play a key role in both biotic and abiotic stress responses.
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)Ph.D. the Program Foundation of Ministry of Education of China (Grant No.20020307036)+1 种基金the Natural Science Foundation of China (Grant Nos.30771329 and 30800677)the Natural Science Foundation of Zhejiang Province,China (Grant No.Y3080359)
文摘The homozygous T3 transgenic lines with sense OsCBL8 gene and antisense OsCBL8 gene obtained by agro-transformation were used to investigate the function of OsCBL8 in rice. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic lines, and decreased to some extents in antisense transgenic lines. Such up- and down-regulation of the OsCBL8 gene in these transgenic lines had little effects on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some of transgenic lines. By evaluation of the tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, and relevant physiological indices, 8F12, a sense transgenic line with high salt tolerance, and 8R14, an antisense transgenic line with high drought tolerance, were obtained, which suggests that the OsCBL8 gene is involved in the response of rice to abiotic stresses.
基金supported by the National Natural Science Foundation of China (31271746, 31401403, 31501366,31201237)
文摘The vacuolar proton pump ATPase（V-H^＋-ATPase）, which is a multi-subunit membrane protein complex, plays a major role in the activation of ion and nutrient transport and has been suggested to be involved in several physiological processes, such as cell expansion and salt tolerance. In this study, three genes encoding V-H^＋-ATPase subunits B（Sc VHA-B, Gen Bank： JF826506）, C（Sc VHA-C, Gen Bank： JF826507） and H（Sc VHA-H, Gen Bank： JF826508） were isolated from the halophyte Suaeda corniculata. The transcript levels of Sc VHA-B, Sc VHA-C and Sc VHA-H were increased by salt, drought and saline-alkali treatments. V-H^＋-ATPase activity was also examined under salt, drought and saline-alkali stresses. The results showed that V-H^＋-ATPase activity was correlated with salt, drought and saline-alkali stress. Furthermore, V-H^＋-ATPase subunits B, C and H（Sc VHA-B, Sc VHA-C and Sc VHA-H） from S. corniculata were introduced separately into the alfalfa genome. The transgenic alfalfa was verified by Southern and Northern blot analysis. During salt and saline-alkali stresses, transgenic lines carrying the B, C and H subunits had higher germination rates than the wild type（WT）. More free proline, higher superoxide dismutase（SOD） activity and lower malondialdehyde（MDA） levels were detected in the transgenic plants under salt and saline-alkali treatments. Moreover, the Sc VHA-B transgenic lines showed greater tolerance to salt and saline-alkali stresses than the WT. These results suggest that overexpression of Sc VHA-B, Sc VHA-C and Sc VHA-H improves tolerance to salt and saline-alkali stresses in transgenic alfalfa.
