目的探究间歇无氧运动对正常体重及超重哮喘患儿呼吸功能和运动能力的影响,为制订针对性的运动处方提供依据。方法选取2015年1月至2017年10月于本院就诊的2640例哮喘患儿为研究对象,根据体质指数(body mass index,BMI)将入选患儿分为超...目的探究间歇无氧运动对正常体重及超重哮喘患儿呼吸功能和运动能力的影响,为制订针对性的运动处方提供依据。方法选取2015年1月至2017年10月于本院就诊的2640例哮喘患儿为研究对象,根据体质指数(body mass index,BMI)将入选患儿分为超重组(BMI>24 kg/m^2,1176例)和正常体重组(BMI≤24 kg/m2,1464例),均进行8周间歇无氧运动。比较两组患儿运动前后呼吸功能和运动能力的变化、无氧运动训练情况及不良反应发生情况。结果两组患儿训练完成率及不良反应发生率比较均无显著差异(χ~2=0.346,P=0.852;χ~2=2.711,P=0.100)。与训练前比较,两组患儿训练后呼气流量峰值(peak expiratory flow,PEF),峰值功率、平均功率及疲劳指数(fatigue index,FI)均显著升高(P<0.05),正常体重组患儿PEF和FI均显著高于超重组(P<0.05),用力肺活量(forced vital capacity,FVC)、第一秒用力呼气量(forced expiratory volume in first second,FEV1)及FEV1/FVC训练前后均无显著差异(P>0.05)。结论间歇无氧运动可明显改善哮喘患儿的呼吸功能和运动能力,尤其对正常体重患儿的改善效果更加明显,同时具有较高的安全性。展开更多
To detect the mammalian target of rapamycin （mTOR） expressed in Cashmere goat fetal fibroblasts （GFb）, mTOR gene was cloned from Inner Mongolia Cashmere goat （Capra hircus） and expressed in Escherichia coli foll...To detect the mammalian target of rapamycin （mTOR） expressed in Cashmere goat fetal fibroblasts （GFb）, mTOR gene was cloned from Inner Mongolia Cashmere goat （Capra hircus） and expressed in Escherichia coli followed by immunizing mice with the purified recombinant protein as an irnmunogen to produce the anti-goat mTOR recombinant polyclonal antibody. Antiserum was collected from the immunized mice after the fifth immunization and its titer was determined with enzyme-linked immunosorbent assay （ELISA）. The results showed that the recombinant polyclonal antibody had a titer 1：200000 and could react with the roTOR expressed in GFb cells with a specific and sensitive affinity. Western blot showed that mTOR expression and phospho-mTOR （Ser 2448） activity were inhibited when GFb cells were treated with CCI-779, an mTOR specific inhibitor.展开更多
文摘目的探究间歇无氧运动对正常体重及超重哮喘患儿呼吸功能和运动能力的影响,为制订针对性的运动处方提供依据。方法选取2015年1月至2017年10月于本院就诊的2640例哮喘患儿为研究对象,根据体质指数(body mass index,BMI)将入选患儿分为超重组(BMI>24 kg/m^2,1176例)和正常体重组(BMI≤24 kg/m2,1464例),均进行8周间歇无氧运动。比较两组患儿运动前后呼吸功能和运动能力的变化、无氧运动训练情况及不良反应发生情况。结果两组患儿训练完成率及不良反应发生率比较均无显著差异(χ~2=0.346,P=0.852;χ~2=2.711,P=0.100)。与训练前比较,两组患儿训练后呼气流量峰值(peak expiratory flow,PEF),峰值功率、平均功率及疲劳指数(fatigue index,FI)均显著升高(P<0.05),正常体重组患儿PEF和FI均显著高于超重组(P<0.05),用力肺活量(forced vital capacity,FVC)、第一秒用力呼气量(forced expiratory volume in first second,FEV1)及FEV1/FVC训练前后均无显著差异(P>0.05)。结论间歇无氧运动可明显改善哮喘患儿的呼吸功能和运动能力,尤其对正常体重患儿的改善效果更加明显,同时具有较高的安全性。
基金supported by the National Natural Science Foundation of China (30860191)the Graduate Student Research Projects of Inner Mongolia University and Major Projects for New Varieties of Genetically Modified Organisms of China (2008ZX08008-002)
文摘To detect the mammalian target of rapamycin （mTOR） expressed in Cashmere goat fetal fibroblasts （GFb）, mTOR gene was cloned from Inner Mongolia Cashmere goat （Capra hircus） and expressed in Escherichia coli followed by immunizing mice with the purified recombinant protein as an irnmunogen to produce the anti-goat mTOR recombinant polyclonal antibody. Antiserum was collected from the immunized mice after the fifth immunization and its titer was determined with enzyme-linked immunosorbent assay （ELISA）. The results showed that the recombinant polyclonal antibody had a titer 1：200000 and could react with the roTOR expressed in GFb cells with a specific and sensitive affinity. Western blot showed that mTOR expression and phospho-mTOR （Ser 2448） activity were inhibited when GFb cells were treated with CCI-779, an mTOR specific inhibitor.
